顺铂致聋后NeuroD在耳蜗螺旋神经节的修复作用研究
发布时间：2018-10-11 07:17
【摘要】：目的检测顺铂损伤后，神经分化因子NeuroD在大鼠耳蜗螺旋神经节的表达变化，探讨其在螺旋神经节损伤后的修复作用研究。 方法成年SD雄性大鼠64只，按随机数随机分为4组，分别为：对照组：按体重以0.9％生理盐水5ml/kg腹腔注射，连续5天，1次/天，在第6天处死；用药1天组：按体重以顺铂（山东齐鲁）5mg/kg腹腔注射，在第2天处死；用药3天组：按体重以顺铂（山东齐鲁）5mg/kg腹腔注射，连续3天，1次/天，在第4天处死；用药5天组：按体重以顺铂（山东齐鲁）5mg/kg腹腔注射，连续5天，1次/天，，在第6天处死。每组16只，建立顺铂耳毒性模型。通过HE染色、免疫荧光染色、免疫组织化学染色、免疫印迹（WesternBlot）及实时定量PCR（Real-Time PCR）等手段，检测用顺铂造聋的耳蜗螺旋神经节损伤后不同的时间点NeuroD的表达变化。 结果成功建立顺铂耳毒性大鼠模型，随着用药时间的延长，神经分化因子NeuroD在耳蜗螺旋神经节中呈动态变化。NeuroD的mRNA表达在用药1天及3天组，与对照组及用药5d组比较差异具有统计学意义（P值均0.01）；免疫组化图片光密度检测结果显示用药1天组及3天组与对照组比较具有统计学意义(P0.01)，用药5天组与对照组比较无统计学意义（P0.05）；Western Blot结果显示用药1天组及3天组与对照组比较具有统计学意义(P0.01)，用药5天组与对照组比较无统计学意义（P0.05）。 结论NeuroD在用药1天后开始增加，3天后达到高峰，5天后下降；在用药早期有一过性表达增强，后期表达下降同时听力损失明显。表明NeuroD可能参与顺铂损伤螺旋神经节后的修复过程。
[Abstract]:Objective to investigate the expression of differentiation factor NeuroD in cochlear spiral ganglion (SCG) after cisplatin injury. Methods Sixty-four adult SD male rats were randomly divided into four groups: control group: the control group was injected intraperitoneally with 0.9% normal saline 5ml/kg for 5 days, once a day, and was killed on the 6th day. The rats in the treatment group were given intraperitoneal injection of cisplatin (Shandong Qilu) 5mg/kg by intraperitoneal injection on the second day, and those in the 3-day group received intraperitoneal injection of cisplatin (Shandong Qilu) 5mg/kg according to body weight for 3 days, once a day, and executed on the 4th day. In the 5-day group, cisplatin (Qilu, Shandong) 5mg/kg was injected intraperitoneally for 5 days, once a day, and executed on the 6th day. The ototoxicity model of cisplatin was established with 16 rats in each group. The expression of NeuroD at different time points after cochlear helical ganglion injury induced by cisplatin was detected by means of HE staining, immunofluorescence staining, immunohistochemical staining, immunoblotting (WesternBlot) and real-time quantitative PCR (Real-Time PCR). Results the rat model of cisplatin ototoxicity was successfully established. With the prolongation of administration time, the neural differentiation factor NeuroD was dynamically changed in the cochlear spiral ganglion. The mRNA expression of NeuroD was observed on the 1st and 3rd day of administration. There was significant difference between the two groups (P < 0.01). The results of light density detection showed that the light density of the treatment group and the 3 day group were significantly higher than that of the control group (P0.01), but there was no significant difference between the 5-day group and the control group (P0.05). The results of Western Blot showed that there was significant difference between the 1 day group and 3 day group compared with the control group (P0.01), but there was no significant difference between the 5 day group and the control group (P0.05). Conclusion NeuroD began to increase after 1 day treatment, reached its peak after 3 days, and decreased after 5 days. The results suggest that NeuroD may be involved in the repair of spiral ganglion injured by cisplatin.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R764
本文编号：2263327
[Abstract]:Objective to investigate the expression of differentiation factor NeuroD in cochlear spiral ganglion (SCG) after cisplatin injury. Methods Sixty-four adult SD male rats were randomly divided into four groups: control group: the control group was injected intraperitoneally with 0.9% normal saline 5ml/kg for 5 days, once a day, and was killed on the 6th day. The rats in the treatment group were given intraperitoneal injection of cisplatin (Shandong Qilu) 5mg/kg by intraperitoneal injection on the second day, and those in the 3-day group received intraperitoneal injection of cisplatin (Shandong Qilu) 5mg/kg according to body weight for 3 days, once a day, and executed on the 4th day. In the 5-day group, cisplatin (Qilu, Shandong) 5mg/kg was injected intraperitoneally for 5 days, once a day, and executed on the 6th day. The ototoxicity model of cisplatin was established with 16 rats in each group. The expression of NeuroD at different time points after cochlear helical ganglion injury induced by cisplatin was detected by means of HE staining, immunofluorescence staining, immunohistochemical staining, immunoblotting (WesternBlot) and real-time quantitative PCR (Real-Time PCR). Results the rat model of cisplatin ototoxicity was successfully established. With the prolongation of administration time, the neural differentiation factor NeuroD was dynamically changed in the cochlear spiral ganglion. The mRNA expression of NeuroD was observed on the 1st and 3rd day of administration. There was significant difference between the two groups (P < 0.01). The results of light density detection showed that the light density of the treatment group and the 3 day group were significantly higher than that of the control group (P0.01), but there was no significant difference between the 5-day group and the control group (P0.05). The results of Western Blot showed that there was significant difference between the 1 day group and 3 day group compared with the control group (P0.01), but there was no significant difference between the 5 day group and the control group (P0.05). Conclusion NeuroD began to increase after 1 day treatment, reached its peak after 3 days, and decreased after 5 days. The results suggest that NeuroD may be involved in the repair of spiral ganglion injured by cisplatin.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R764
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