Bmi-1在脉络膜黑色素瘤中的表达及其与P16、Ki67的相关性分析

发布时间：2019-01-04 21:06

【摘要】：目的观察Bmi-1、P16和Ki67在脉络膜黑色素瘤中的表达,并对Bmi-1、Ki67和Bmi-1、P16分别作相关性分析,以明确Bmi-1与脉络膜黑色素瘤恶性程度的关系,并初步探讨了Bmi-1的作用机制。方法采用免疫组织化学法检测20例脉络膜黑色素瘤组织及20例色素痣组织中Bmi-1、P16和Ki67的表达,与临床病理资料做对照,并对脉络膜黑色素瘤组中Bmi-1和Ki67、Bmi-1和P16的表达作相关性分析。结果Bmi-1、P16及Ki67蛋白在脉络膜黑色素瘤中的表达率分别为65%,30%,75%。在色素痣中的表达率分别为70%、95%、20%。与色素痣组相比,Bmi-1和Ki67在脉络膜黑色素瘤中的强阳性表达率增加,差异有统计学意义(x2=7.619,P=0.006；x2=13.333,P=0.000)；P16蛋白在脉络膜黑色素瘤中的表达降低,差异有统计学意义(x2=18.027,P=0.000)。Bmi-1蛋白在脉络膜黑色素瘤组不同组织学类型中的阳性表达率分别为：梭形细胞型33.3%,类上皮型100%,混合型80%,差异有统计学意义(x2=9.745,P=0.008)。Bmi-1蛋白在不同大小的脉络膜黑色素瘤中的表达为：大肿瘤(83.33%),中等大小肿瘤(87.50%)和小肿瘤(16.67%),差异具有统计学意义(x2=8.828,P=0.012)。Bmi-1蛋白在脉络膜黑色素瘤中累及巩膜组和未累及巩膜组的阳性表达率分别为90.81%、33.33%,差异有统计学意义(x2=7.213,P=0.027)。脉络膜黑色素瘤组Bmi-1与Ki67的表达呈正相关(x2=17.786,P=0.038),Bmi-1与P16的表达呈负相关,差异有统计学意义(x2=15.918,P=0.014)。结论 1.脉络膜黑色素瘤中Bmi-1高表达和P16低表达,提示Bmi-1和P16可能都参与了脉络膜黑色素瘤的发生发展。 2.脉络膜黑色素瘤累及巩膜组Bmi-1基因较未累及组表达增加,提示Bmi-1与脉络膜黑色素瘤的侵袭转移密切相关。 3.Bmi-1基因在不同大小和不同分型的脉络膜黑色素瘤中的表达不同,提示可能与肿瘤增殖和预后有关。 4.脉络膜黑色素瘤组中Bmi-1和Ki67的表达呈正相关,提示Bmi-1基因与脉络膜黑色素瘤的恶性程度密切相关。 5.脉络膜黑色素瘤组中Bmi-1和P16的表达呈负相关,提示Bmi-1可能是通过抑制P16的作用促进肿瘤的发生,但具体机制尚需进一步研究。
[Abstract]:Objective to observe the expression of Bmi-1,P16 and Ki67 in choroidal melanoma and to analyze the correlation between Bmi-1,Ki67 and Bmi-1,P16 in order to clarify the relationship between Bmi-1 and malignant degree of choroidal melanoma. The mechanism of Bmi-1 was also discussed. Methods Immunohistochemical method was used to detect the expression of Bmi-1,P16 and Ki67 in 20 cases of choroidal melanoma and 20 cases of nevus pigmentosa. The expression of Bmi-1 and Ki67, in choroidal melanoma was compared with the clinicopathological data. The correlation between the expression of Bmi-1 and P16 was analyzed. Results the expression rates of Bmi-1,P16 and Ki67 protein in choroidal melanoma were 65% and 30% respectively. The expression rates in nevus pigmentosa were 70 and 95%, respectively. Compared with nevus group, the strong positive expression rate of Bmi-1 and Ki67 in choroidal melanoma was significantly higher than that in nevus group. The expression of P16 protein in choroidal melanoma was significantly lower than that in choroidal melanoma (x2 + 18.027). The positive expression rates of Bmi-1 protein in different histological types of choroidal melanoma group were as follows: spindle cell type 33.33.3, epithelioid type 100th, mixed type 80th, the difference was statistically significant (x2P 9.745). The expression of Bmi-1 protein in choroidal melanoma was 83.33% (large tumor), 87.50% (medium size tumor) and 16.67% (small tumor), and the difference was statistically significant (x28.828), the expression of Bmi-1 protein in choroidal melanoma was 83.33%, 87.50% and 16.67% respectively. The positive expression rates of Bmi-1 protein in choroidal melanoma were 90.81% and 33.33%, respectively (x27.213). There was a positive correlation between Bmi-1 and Ki67 expression in choroidal melanoma group (x2p21. 786), and a negative correlation between Bmi-1 and P16 expression. The difference was statistically significant (x2 + 15. 918, P0. 014). Conclusion 1. The high expression of Bmi-1 and the low expression of P16 in choroidal melanoma suggest that both Bmi-1 and P16 may be involved in the development of choroidal melanoma. 2. The expression of Bmi-1 gene was increased in choroidal melanoma with scleral involvement, suggesting that Bmi-1 was closely related to the invasion and metastasis of choroidal melanoma. The expression of 3.Bmi-1 gene in choroidal melanoma of different sizes and types may be related to tumor proliferation and prognosis. 4. There was a positive correlation between the expression of Bmi-1 and Ki67 in choroidal melanoma, suggesting that Bmi-1 gene was closely related to the malignant degree of choroidal melanoma. 5. The expression of Bmi-1 and P16 in choroidal melanoma was negatively correlated, suggesting that Bmi-1 may promote the development of tumor by inhibiting P16, but the specific mechanism needs to be further studied.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.7

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