人参多糖对耐顺铂人鼻咽癌细胞耐药的逆转作用及其机制

发布时间：2019-07-23 18:46

【摘要】：目的探讨人参多糖(GPS)对耐顺铂人鼻咽癌细胞耐药的逆转作用及机制。方法选择药物敏感鼻咽癌细胞CNE-2作为亲本细胞,并建立耐顺铂的鼻咽癌耐药细胞株系CNE-2(简称耐药CNE-2)。采用MTT法检测亲本细胞及耐药CNE-2经GPS作用前后对顺铂的半数抑制浓度(IC50),并计算耐药指数(RI);流式细胞仪检测GPS对耐药CNE-2的增殖抑制率;RT-PCR法检测GPS作用前后耐药CNE-2中β-catenin mRNA表达;显微镜观察GPS作用前后耐药CNE-2中β-catenin蛋白表达。结果亲本细胞及耐药CNE-2对顺铂的IC50分别为(0.15±0.11)、(7.50±2.80)μmol/L,耐药CNE-2对顺铂的RI为26.8;0.1、0.2、0.3、0.4 g/L GPS作用48 h,耐药CNE-2对顺铂的IC50分别为(3.21±1.12)、(1.14±0.53)、(0.64±0.38)、(0.21±0.17)μmol/L,RI分别为9.5、6.4、3.2、1.2,GPS作用后耐药CNE-2对顺铂IC50、RI均较作用前明显降低(P均0.05)。GPS对耐药CNE-2的增殖具有明显抑制作用,且呈剂量和时间依赖性(P均0.05)。0.1、0.2、0.3、0.4 g/L GPS处理48 h,耐药CNE-2中β-catenin mRNA相对表达量分别为0.973±0.114、0.809±0.038、0.706±0.050、0.297±0.010,与耐药CNE-2比较,0.3 g/L及0.4 g/L GPS作用后β-catenin mRNA相对表达量均明显降低(P均0.05)。GPS作用前β-catenin蛋白的表达区域在细胞核以及细胞质,经GPS处理后,β-catenin蛋白表达区域逐渐向细胞膜转移。结论 GPS可逆转CNE-2对顺铂的耐药性;其机制可能为通过β-catenin信号转导途径诱导耐药株系凋亡。
[Abstract]:Objective to investigate the reversal effect and mechanism of ginseng polysaccharide (GPS) on drug resistance of human nasopharyngeal carcinoma cells resistant to platinum. Methods Drug-sensitive nasopharyngeal carcinoma cell line CNE-2 was selected as parent cell line, and a platinum-resistant nasopharyngeal carcinoma cell line CNE-2 (drug-resistant CNE-2) was established. MTT assay was used to detect the half inhibitory concentration (IC50) of parent cells and drug-resistant CNE-2 to DDP before and after treatment with GPS, and the proliferation inhibition rate of GPS to drug-resistant CNE-2 was detected by (RI); flow cytometry, the expression of 尾-catenin mRNA in drug-resistant CNE-2 before and after treatment with GPS was detected by RT-PCR method, and the expression of 尾-catenin protein in drug-resistant CNE-2 before and after treatment with GPS was observed by microscope. Results the IC50 of parental cells and drug-resistant CNE-2 to DDP was (0.15 卤0.11), (7.50 卤2.80) 渭 mol/L, respectively. The RI of drug-resistant CNE-2 to DDP was 26.8. 0.1, 0.2, 0.3, 0.4 g / L GPS for 48 h, the IC50 of drug-resistant CNE-2 was (3.21 卤1.12), (1.14 卤0.53), (0.64 卤0.38), (0.21 卤0.17) 渭 mol/L,RI was 9.5, 6.4, 1.2, respectively. The drug-resistant CNE-2 had a significant decrease in the proliferation of DDP IC50,RI compared with that before treatment. GPS had a significant inhibitory effect on the proliferation of drug-resistant CNE-2. The relative expression of 尾-catenin mRNA in drug-resistant CNE-2 was 0.973 卤0.114, 0.809 卤0.038, 0.706 卤0.050,0.297 卤0.010, respectively, compared with drug-resistant CNE-2, and the relative expression of 尾-catenin mRNA in drug-resistant CNE-2 was 0.973 卤0.114, 0.809 卤0.038, 0.706 卤0.050,0.297 卤0.010, respectively. The relative expression of 尾-catenin mRNA was significantly decreased after treatment with 0.3g / L and 0.4g / L GPS. The expression region of 尾-catenin protein was in the nucleus and cytoplasm before GPS treatment. After GPS treatment, the expression region of 尾-catenin protein gradually transferred to the cell membrane. Conclusion GPS can reverse the resistance of CNE-2 to DDP, and its mechanism may be to induce apoptosis of drug-resistant strains through 尾-catenin signal transduction pathway.
【作者单位】：第二军医大学学员旅;
【分类号】：R739.63

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