α-硫辛酸对慢性心力衰竭大鼠心功能的影响

发布时间：2017-12-26 16:44

本文关键词：α-硫辛酸对慢性心力衰竭大鼠心功能的影响　出处：《重庆医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的探讨α-硫辛酸(α-lipoic acid)抗氧化作用及抗心肌细胞凋亡对慢性心力衰竭大鼠心功能的影响。方法用急性心肌梗死(AMI)后大鼠心功能异常的方法构建慢性心力衰竭(CHF)模型,6周龄的雄性SD大鼠随机分为α-硫辛酸处理组(AMI+α-硫辛酸,n=15)、对照组(AMI+NS,n=14)和假手术组(sham-operated+NS,n=10)。术后2周后α-硫辛酸处理组给予硫辛酸(60mg/kg/d)腹腔注射,其余组大鼠等量生理盐水(NS)腹腔注射。4周后,采用超声心动图分析各组大鼠的心功能、ELISA法检测血浆脑钠尿肽(BNP)含量、超氧化物歧化酶(SOD)、总抗氧化能力(TAC)、丙二醛(MDA)含量、HE染色观察心肌细胞形态、免疫组化法分析心肌诱导型氧化亚氮合酶(i NOS)的表达、Western blot法测大鼠心肌组织c-Jun氨基末端激酶(JNK)及Caspase-12蛋白含量。结果对照组与假手术组大鼠相比,大鼠心功能明显降低(射血分数由90.16%降至66.84%),血清BNP、MDA水平明显升高(P0.05),SOD、TAC水平降低(P0.05);HE染色显示心肌组织明显水肿、排列紊乱,iNOS表达升高(P0.05),证明CHF建模成功;α-硫辛酸处理组与对照组相比,大鼠的心功能明显改善(射血分数由66.84%升至80.15%),BNP、MDA含量、iNOS、JNK、Caspase-12蛋白表达均下降(P0.05),TAC、ROS水平升高(P0.05)。结论CHF建模成功;α-硫辛酸可通过抗氧化和减少细胞凋亡的方式改善CHF大鼠的心功能。
[Abstract]:Objective to investigate the effects of alpha lipoic acid (alpha -lipoic acid) on the cardiac function of rats with chronic heart failure (CHF) and the antioxidation of alpha lipoic acid (alpha acid). Methods a model of chronic heart failure (CHF) was established after cardiac dysfunction in rats with acute myocardial infarction (AMI). 6 week old male SD rats were randomly divided into alpha lipoic acid treatment group (AMI+ alpha lipoic acid, n=15), control group (AMI+NS, n=14) and sham operation group (sham-operated+NS, n=10). 2 weeks after operation, alpha lipoic acid treatment group was given intraperitoneal injection of lipoic acid (60mg/kg/d), and the rest of the rats were injected with equal amount of normal saline (NS). After 4 weeks, the echocardiography analysis of rats cardiac function, ELISA method for detection of plasma brain natriuretic peptide (BNP) content, superoxide dismutase (SOD), total antioxidant capacity (TAC), malondialdehyde (MDA) content, HE staining was used to observe the myocardial cell morphology and immunohistochemical analysis of myocardium inducible synthase (I NOS) Nitrous Oxide c-Jun N-terminal kinase measured in myocardial tissue of rats and the expression of Western blot (JNK) and Caspase-12 protein content. Results compared with control group and sham operation group rats, the heart function of rats decreased significantly (ejection fraction decreased from 90.16% to 66.84%), serum BNP and MDA levels were significantly increased (P0.05), SOD and TAC were decreased (P0.05); HE staining of myocardial tissue edema, disorder, increase the expression of iNOS (P0.05) CHF, proved successful modeling; compared with alpha lipoic acid treatment group and control group, the heart function of rats significantly improved (ejection fraction from 66.84% to 80.15%), the expression of BNP, MDA, iNOS, JNK content and Caspase-12 protein were decreased (P0.05), elevated TAC and ROS levels (P0.05). Conclusion CHF is successful in modeling, and alpha lipoic acid can improve the cardiac function of CHF rats by antioxidation and reduction of apoptosis.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R541.6

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