新疆哈萨克族高血压病患者外周血T淋巴细胞NFAT信号通路相关分子及其药物干预的实验研究

发布时间：2018-01-13 06:12

本文关键词：新疆哈萨克族高血压病患者外周血T淋巴细胞NFAT信号通路相关分子及其药物干预的实验研究　出处：《新疆医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:研究新疆哈萨克族高血压病患者外周血T淋巴细胞中NFAT信号通路的活化情况,并观察替米沙坦干预前后NFAT信号通路及相关炎症因子的表达。方法:通过读取随机数字表的方法随机选取60例首次就诊且未经药物治疗的新疆哈萨克族高血压病患者,其中30例作为高血压组,30例为替米沙坦药物干预组,同时选取30例哈萨克族健康体检者作为对照组。每位入选者均肘静脉采血10ml,采用密度梯度离心法分离出单个核细胞,再经免疫磁珠法分离3组患者外周血T淋巴细胞增殖培养48h后采用替米沙坦100umol/L对干预组干预72h,其余两组不添加药物。每受试样本增殖培养的T淋巴细胞分成两份,分别提取总RNA和蛋白,运用荧光定量PCR和Western blot技术检测三组患者T淋巴细胞NFATc1与其下游信号分子IL-6、TNF-α的mRNA和蛋白的相对表达量。结果:两组受试者基线差异无统计学意义。(1)与对照组相比,高血压组的NFATc1、TNF-α及IL-6的mRNA和蛋白表达水平均有明显升高(P0.05)。(2)与高血压组相比,经替米沙坦药物干预后的NFATc1、TNF-α及IL-6的mRNA和蛋白表达水平均下调(P0.05)。(3)和对照组相比,经替米沙坦药物干预后的NFATc1、TNF-α及IL-6的mRNA表达水平稍有升高(其中NFATc1指标P0.05,后两者P0.05)。(4)和对照组相比,经替米沙坦药物干预后NFATc1蛋白表达量较对照组而言有轻微升高(P0.05),而TNF-α及IL-6的蛋白表达量有所降低(P0.05)。结论:新疆哈萨克族高血压患者外周血T淋巴细胞中存在NFAT信号通路的激活,从而参于高血压的低级别炎症的病理过程,而替米沙坦可能通过减少NFATc1的释放而降低炎性因子的生成。
[Abstract]:Objective: to study the activation of NFAT signal pathway in peripheral blood T lymphocytes of Kazak patients with hypertension in Xinjiang. The expression of NFAT signaling pathway and related inflammatory factors were observed before and after telmisartan intervention. By reading the random number table, 60 cases of Xinjiang Kazak patients with hypertension were randomly selected who had not been treated with drugs for the first time. 30 cases as hypertension group 30 cases as telmisartan drug intervention group and 30 Kazak healthy people as control group. Mononuclear cells were isolated by density gradient centrifugation. The peripheral blood T lymphocytes of the three groups were separated by immunomagnetic beads for 48 h, then treated with telmisartan 100 umol / L for 72 h. The other two groups did not add drugs. Each T lymphocyte cultured by the sample was divided into two parts and the total RNA and protein were extracted respectively. The T lymphocyte NFATc1 and its downstream signal molecule IL-6 were detected by fluorescence quantitative PCR and Western blot. Results: there was no significant difference in baseline between the two groups. Results: compared with the control group, the NFATc1 in the hypertension group was higher than that in the control group. The expression levels of mRNA and protein in TNF- 伪 and IL-6 were significantly higher than those in hypertension group. The expression of mRNA and protein in TNF- 伪 and IL-6 were down-regulated (P0.05A) compared with the control group, the NFATc1 treated with telmisartan. The expression of mRNA in TNF- 伪 and IL-6 was slightly higher than that in the control group (NFATc1 index P0.05, the latter two P0.05A. 4). The expression of NFATc1 protein after telmisartan intervention was slightly higher than that of control group (P0.05). However, the protein expression of TNF- 伪 and IL-6 was decreased (P 0.05). Conclusion: there is activation of NFAT signal pathway in peripheral blood T lymphocytes of Kazakh patients with hypertension in Xinjiang. Thus, it is involved in the pathological process of low grade inflammation in hypertension, and telmisartan may reduce the production of inflammatory factors by reducing the release of NFATc1.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R544.1

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