心室内注射人脐带间充质干细胞改善心肌梗死大鼠心功能及作用机制

发布时间：2018-02-03 12:00

本文关键词：心肌梗塞脐带间质干细胞移植粒细胞巨噬细胞集落刺激因子转化生长因子β 组织工程干细胞移植人脐带间充质干细胞心肌梗死粒细胞-巨噬细胞集落刺激因子心功能　出处：《中国组织工程研究》2017年25期 　论文类型：期刊论文

【摘要】：背景:人脐带间充质干细胞具有分化潜力大,增殖能力强,免疫原性较低等特点,在创伤修复、局部缺血组织的血管再生等方面发挥不可估量的作用。目的:探讨心室内注射人脐带间充质干细胞对心肌梗死大鼠心肌组织粒细胞-巨噬细胞集落刺激因子、转化生长因子β1表达的影响。方法:选取成年雄性健康SD大鼠120只,随机选取20只为健康对照组,余100只于腹腔皮下多点注射异丙肾上腺素建立心肌梗死大鼠模型。造模成功后随机分为模型组、人脐带间充质干细胞上清液组、人脐带间充质干细胞低剂量组、中剂量组及高剂量组,每组20只。造模后24 h,分别将生理盐水注射液、人脐带间充质干细胞上清液、含0.25×10~6个,1.0×10~6个,4.0×10~6个人脐带间充质干细胞悬液2 mL缓慢注入心室。治疗2周后应用心脏超声测定左室射血分数、收缩末期左室容积及舒张末期左室容积。ELISA法测定血清乳酸脱氨酶、肌酸激酶及脑钠肽前体水平。取心脏标本,硝基四氮唑蓝染色法测定心肌梗死面积,Western blot法测定心肌组织粒细胞-巨噬细胞集落刺激因子、转化生长因子β1表达。结果与结论:(1)人脐带间充质干细胞低、中、高剂量组左室射血分数高于模型组及人脐带间充质干细胞上清液组,而收缩末期左室容积、舒张末期左室容积低于模型组及人脐带间充质干细胞上清液组(P0.05),人脐带间充质干细胞中、高剂量组上述指标优于低剂量组(P0.05);(2)人脐带间充质干细胞低、中、高剂量组血清乳酸脱氨酶、肌酸激酶及脑钠肽前体水平及心肌组织粒细胞-巨噬细胞集落刺激因子、转化生长因子β1表达均低于模型组及人脐带间充质干细胞上清液组(P0.05),而人脐带间充质干细胞中、高剂量组上述指标低于低剂量组(P0.05);(3)人脐带间充质干细胞低、中、高剂量组心肌梗死面积小于模型组及人脐带间充质干细胞上清液组(P0.05),而人脐带间充质干细胞中、高剂量组心肌梗死面积小于低剂量组(P0.05);(4)结果表明,人脐带间充质干细胞能明显降低心肌梗死大鼠心肌组织粒细胞-巨噬细胞集落刺激因子、转化生长因子β1表达,进而有效保护心肌组织,改善心功能。
[Abstract]:Background: human umbilical cord mesenchymal stem cells have the characteristics of great differentiation potential, strong proliferative ability and low immunogenicity. Objective: to investigate the effects of intraventricular injection of human umbilical cord mesenchymal stem cells on myocardial granulocyte-macrophage colony stimulating factor in myocardial infarction rats. Methods: 120 adult male healthy SD rats were selected and 20 healthy rats were randomly selected as healthy control group. The other 100 rats were injected with isoproterenol into the abdominal cavity to establish myocardial infarction model. After the establishment of the model, the rats were randomly divided into model group and human umbilical cord mesenchymal stem cell supernatant group. Human umbilical cord mesenchymal stem cells in low dose group, medium dose group and high dose group, 20 rats in each group. 24 hours after modeling, saline injection, human umbilical cord mesenchymal stem cells supernatant. It contains 0.25 脳 10 ~ 6 ~ 1. 0 脳 10 ~ 6. 4. 0 脳 10 6 human umbilical cord mesenchymal stem cell suspension 2 mL was injected into the ventricle slowly. After 2 weeks of treatment, left ventricular ejection fraction (LVEF) was measured by echocardiography. The serum levels of lactate deaminase, creatine kinase and brain natriuretic peptide precursor were measured by Elisa and left ventricular volume at the end of systolic and diastolic period respectively. Myocardial infarction area was determined by nitrotetrazolium blue staining and the granulocyte-macrophage colony stimulating factor was determined by blot. Results and conclusion Human umbilical cord mesenchymal stem cells were low, and the left ventricular ejection fraction in high dose group was higher than that in model group and human umbilical cord mesenchymal stem cell supernatant group. The left ventricular volume at the end of systolic and diastolic period was lower than that in the model group and the human umbilical cord mesenchymal stem cell supernatant group (P0.05), and in the human umbilical cord mesenchymal stem cells. The above indexes in the high dose group were better than those in the low dose group (P 0.05). The levels of serum lactate deaminase, creatine kinase and brain natriuretic peptide precursor and myocardial granulocyte-macrophage colony stimulating factor were low in human umbilical cord mesenchymal stem cells. The expression of transforming growth factor 尾 1 was lower than that of model group and human umbilical cord mesenchymal stem cell supernatant group (P0.05), but in human umbilical cord mesenchymal stem cells. The above indexes in the high dose group were lower than those in the low dose group (P 0.05). 3) Human umbilical cord mesenchymal stem cells were low, and the myocardial infarction size in high dose group was smaller than that in model group and human umbilical cord mesenchymal stem cell supernatant group (P0.05), while in human umbilical cord mesenchymal stem cell group. The area of myocardial infarction in high dose group was smaller than that in low dose group (P 0.05). The results showed that human umbilical cord mesenchymal stem cells could significantly reduce the expression of granulocyte-macrophage colony stimulating factor and transforming growth factor 尾 1 in myocardial tissue of rats with myocardial infarction. Improve cardiac function.
【作者单位】：南阳医学高等专科学校第一附属医院心血管外科;
【分类号】：R542.22
【正文快照】： 0引言Introduction复受损心肌细胞的理想细胞来源[10-11]。为此,实验探讨人心肌梗死导致心肌细胞凋亡而引起心脏局部浸润性脐带间充质干细胞对心肌梗死大鼠心肌功能的改善作用及炎症反应,并介导免疫细胞趋化至炎症浸润部位,在清除其作用机制。坏死心肌细胞同时会大量释放炎症

【相似文献】