高浓度弹力蛋白酶不同灌注保持时间对建立兔腹主动脉瘤模型的影响

发布时间：2018-02-12 11:55

本文关键词： 腹主动脉瘤弹力蛋白酶　出处：《中国老年学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的通过经腹主动脉腔内灌注高浓度弹力蛋白酶建立兔腹主动脉瘤(AAA)模型,观察不同灌注保持时间对兔AAA成瘤率及死亡率的影响。方法 24只新西兰雄兔随机分为A组、B组、C组各8只,每组均经双腔导管注入10μl、浓度为100 U/ml的弹力蛋白酶,灌注保持时间A组3~5 min,B组7~10 min,C组20~30 min;后用生理盐水冲洗管腔3次;分别于术前及术后第7天行腹主动脉彩超检查,术后第7天处死动物,行组织学病理染色观察血管灌注部位组织学变化。结果 A组存活8只,灌注局部血管扩张率40.3%~63.1%,其中5只扩张率大于50%,达到AAA成瘤标准,成瘤率62.5%,术后7 d存活率100%;B组存活8只,局部血管扩张率87.5%~109.2%,均达到成瘤标准,成瘤率100%,术后7 d存活率100%;C组存活3只,灌注局部血管扩张率237.7%~252.0%,成瘤率100%,术后7 d存活率为37.5%。结论高浓度弹力蛋白酶制作兔AAA模型,灌注时间保持7~10 min具有成瘤率高、死亡率低、操作简易等优点,易于推广。
[Abstract]:Objective to establish an AAA model of abdominal aortic aneurysm in rabbits by intraluminal perfusion of high concentration elastase through abdominal aorta. Methods 24 New Zealand male rabbits were randomly divided into group A (n = 8), group B (n = 8) and group C (n = 8). The perfusion retention time of group A was 3 minutes 5 minutes, group B was 7 minutes 10 minutes and group C was 20 minutes 30 minutes, and then rinsed the lumen with normal saline for 3 times. The abdominal aorta was examined by color Doppler ultrasonography before and 7 days after operation, and the animals were killed on the 7th day after operation. Results in group A, 8 rats survived, and the local vasodilation rate was 40.3%, 63.1%, 5 of them were larger than 50, which reached the standard of AAA tumorigenesis. The tumor formation rate was 62.5%. The survival rate of group B was 100 on the 7th day after operation. The local vasodilation rate was 87.5% and 109.2%, all of them reached the standard of tumorigenesis, and the tumorigenesis rate was 100%. The survival rate of group C was 100% on the 7th day after operation, and 3 rats in group C survived on the 7th day after operation. The local vasodilation rate was 237.7% and the tumorigenesis rate was 100%. The survival rate was 37.50.Conclusion the AAA model of rabbits made by high concentration elastase has the advantages of high tumorigenesis rate, low mortality, easy operation and easy to be popularized.
【作者单位】：首都医科大学附属北京安贞医院心内科;
【基金】：国家自然科学基金(No.81370437)
【分类号】：R-332;R543.16

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