胰岛素及葡萄糖对SD大鼠内皮细胞表达sES以及平滑肌细胞表达MMP-9、TIMP-1的影响

发布时间：2018-03-01 03:21

本文关键词： 胰岛素葡萄糖 E选择素基质金属蛋白酶9 组织抑制因子-1　出处：《南华大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:研究胰岛素与葡萄糖对SD大鼠内皮细胞表达可溶性E选择素(sES)及平滑肌细胞表达基质金属蛋白酶9(MMP-9)、组织抑制因子-1(TIMP-1)的影响。方法:培养SD大鼠主动脉内皮细胞及颈内动脉平滑肌细胞,用不同浓度的胰岛素和葡萄糖对其进行刺激,以酶联免疫吸附试验(ELISA)分别测定上清液sES、MMP-9及TIMP-1的水平。结果:与对照组(无胰岛素)相比,胰岛素刺激组sES和MMP-9表达水平显著增高(P0.05和P0.05),TIMP-1表达则显著降低(P0.05),且差异有统计学意义。经Pearson相关分析显示,胰岛素浓度与sES(r=0.841,P0.001)及MMP-9(r=0.815,P0.001)呈正相关,与TIMP-1(r=--0.963,P0.001)呈负相关。与对照组(无葡萄糖)相比,葡萄糖刺激组sES和MMP-9表达水平显著增高(P0.005和P0.001),TIMP-1表达则显著降低(P0.001),且差异有统计学意义。经Pearson相关分析显示,葡萄糖浓度与sES(r=0.972,P0.001)及MMP-9(r=0.973,P0.001)呈正相关,与TIMP-1(r=-0.963,P0.001)呈负相关。结论:胰岛素及葡萄糖在一定浓度范围内能促进SD大鼠内皮细胞sES及平滑肌细胞MMP-9表达,抑制平滑肌细胞TIMP-1表达。
[Abstract]:Objective: to study the effects of insulin and glucose on the expression of soluble E selectin ESSS (soluble E selectin ESS) and matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1) in rat endothelial cells. Methods: aortic endothelium of SD rats was cultured in vitro. Cells and smooth muscle cells of the internal carotid artery, The levels of sESU MMP-9 and TIMP-1 in supernatant were determined by enzyme linked immunosorbent assay (Elisa) with different concentrations of insulin and glucose. In insulin-stimulated group, the expression of sES and MMP-9 increased significantly (P0.05) and the expression of TIMP-1 decreased significantly (P 0.05), and the difference was statistically significant. The Pearson correlation analysis showed that the insulin concentration was positively correlated with the expression of sESnr ~ (0.841) P _ (0.001) and MMP-9 ~ (9) (P ~ (0.815) P _ (0.001)). The expression of sES and MMP-9 in glucose stimulated group was significantly higher than that in control group (P 0. 005 and P 0. 001), and the expression of TIMP-1 was significantly lower than that in control group (P 0. 005 and P 0. 001 P 0. 001). The Pearson correlation analysis showed that the expression of sES and MMP-9 in glucose stimulated group was significantly lower than that in control group (P 0. 005 and P 0. 001). There was a positive correlation between glucose concentration and the expression of sES and MMP-9 in the endothelial cells and smooth muscle cells of SD rats, and a negative correlation between the concentration of glucose and TIMP-1 / 0.963P 0.001. Conclusion: insulin and glucose can promote the expression of sES and MMP-9 in smooth muscle cells and inhibit the expression of TIMP-1 in smooth muscle cells of SD rats.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R543

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