MVN区Glu离子型受体参与血清NE含量的调节

发布时间：2018-03-02 23:34

本文选题：急性低血压　切入点：前庭内侧核　出处：《延边大学》2017年硕士论文　论文类型：学位论文

【摘要】：实验目的:1998年就有学者提出,前庭交感反射的中枢通路。它们是前庭神经-前庭神经核(nucleivestibularis,VN)-延髓外侧网状结构-延髓头端腹外侧区-脊髓灰质侧角-交感神经。本研究室用免疫组织化学法又发现,前庭内侧核(medial vestibular nucleus,MVN)区的 N-甲基-D-门冬氨酸受体(N-methy1-D-aspartic acid receptor,NMDA)和α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体(amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor,AMPA)参与由急性低血压诱发的前庭性血压调控过程,也能增加血清肾上腺素的浓度明显增加。机体血液中肾上腺素的来源主要是肾上腺髓质,而去甲肾上腺素则由肾上腺髓质和交感神经节后神经元的末梢分泌。血液中的去甲肾上腺素可以反应交感神经的兴奋状态。至于急性低血压经外周前庭器官影响血压的调控中是否有交感神经参与,未见相关研究报道。本研究的目的在于探讨急性低血压诱发前庭性血压调控过程中,MVN区谷氨酸的部分离子型受体对血清去甲肾上腺素含量的影响。同时采用蛋白印迹分析法进一步证实硝普钠诱发急性低血压时,MVN区给予谷氨酸的离子型受体激动剂和阻断剂对脊髓胸段内c-Fos和p-ERK1/2蛋白表达的影响。以证明急性低血压时MVN区的谷氨酸离子型受体经过脊髓-交感神经参与由急性低血压诱发的前庭性血压调控机制。实验方法:实验为了排除血压的变化经过压力感受器对中枢神经核团功能的影响,用手术方法摘除动物的颈动脉窦和主动脉弓压力感受器。用多通道生理记录仪监测该模型大鼠压力感受性反射的敏感性,确定模型制备成功48h后,再进行MVN区核团定位,将36只SD大鼠随机分为阻断剂组(Antagonist)和激动剂组(Agonist)。阻断剂组中分为MVN区注射人工脑脊液(ACSF)的对照组以及阻断剂MK-801、CNQX,核团给药10min后静脉注射硝普钠(Sodiumnitroprusside,SNP)诱发低血压。激动剂组又分为注射人工脑脊液(ACSF)的对照组以及受体激动剂NMDA组和AMPA组3个小组。激动剂组不诱发急性低血压。用微量泵以1.5μl/min的速度向核团注射药品。30min后开胸心室腔内取血离心获得血清后,用ELASA法检测血清去甲肾上腺素的含量。取T5-7段脊髓,用蛋白印迹分析法(Western-Blot)对该段脊髓内c-Fos和p-ERK1/2蛋白表达进行检测。实验结果:1.将谷氨酸(Glu)离子型受体阻断剂(CNQX、MK-801)经过定位针注射到前庭内侧核(MVN)区后,再用硝普钠(SNP)诱发急性低血压,发现胸髓T5-7段中的c-Fos蛋白的表达以及p-ERK1/2蛋白含量明显低于对照组(ACSF)(P0.05)。2.前庭内侧核区注射谷氨酸(Glu)离子型受体激动剂(NMDA、AMPA),使胸髓T5-7段中的c-Fos和p-ERK1/2蛋白的表达含量明显高于注射人工脑脊液的对照组(P0.05)。3.把谷氨酸(Glu)的两种离子型受体阻断剂(CNQX、MK-801)注射到前庭内侧核(MVN)区后,再用硝普钠(SNP)诱发急性低血压,发现血清中去甲肾上腺素(NE)的含量明显低于对照组(ACSF)(P0.05)。4.前庭内侧核区的谷氨酸(Glu)离子型受体激动剂(NMDA、AMPA),明显提高血清中去甲肾上腺素(NE)的含量,与对照组(ACSF)比较差异显著(P0.05)。结论:诱发急性低血压可以使MVN区谷氨酸离子型受体兴奋再通过兴奋交感神经参与血压的调节。
[Abstract]:Objective: to 1998, some scholars have suggested that the central pathway of vestibular sympathetic reflex. They are vestibular nerve vestibular nucleus (nucleivestibularis, VN) - lateral medullary reticular formation - rostral ventrolateral medulla - spinal cord lateral horn - sympathetic nerve. This study by immunohistochemical method and found that the medial vestibular nucleus (medial vestibular nucleus, MVN) N- methyl -D- aspartate receptor region (N-methy1-D-aspartic acid receptor, NMDA -3-) and alpha amino hydroxy -5- methyl -4- isoxazole propionate receptor (amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor, AMPA) involved in the regulation of blood pressure by vestibular evoked acute hypotension, can also increase the concentration of serum epinephrine increased significantly the source of adrenaline in the blood. The body is mainly the adrenal medulla, and norepinephrine from the adrenal medulla and sympathetic postganglionic nerve The peripheral blood in the secretion of norepinephrine can response to sympathetic nerve stimulation. As for acute hypotension by peripheral vestibular organs influence whether blood pressure regulation of sympathetic nerve involvement, no reports about. The purpose of this study is to investigate the acute hypotension induced by vestibular blood pressure regulation process, part of the ion receptor MVN the excitatory glutamate effect of norepinephrine on the content of serum. At the same time using Western blot analysis further confirmed that acute hypotension induced by sodium nitroprusside, ionic MVN administration of glutamate receptor agonists and blocking agents on the expression of c-Fos and p-ERK1/2 protein in the spinal cord. The ionotropic glutamate proved acute hypotension MVN region of the receptor after spinal cord - sympathetic nerve involvement by the vestibular blood pressure regulation mechanism of induced acute hypotension. Methods: experiments in order to exclude The change of blood pressure after effects on the central nervous nuclei baroreceptor function, carotid sinus and aortic arch baroreceptors with surgical removal of the animal. The feeling reflected by the pressure sensitivity of rat multi channel physiological recorder monitoring the model, determine the model preparation work after 48h, and then MVN nuclei, 36 SD rats were randomly divided into blocker group (Antagonist) and agonist group (Agonist). The blocker group divided into injection of artificial cerebrospinal fluid (ACSF) in the MVN region of the control group and the blocking agent MK-801, CNQX, nucleus after administration of 10min intravenous injection of sodium nitroprusside (Sodiumnitroprusside, SNP) agonist induced hypotension. Group was divided into injection of artificial cerebrospinal fluid (ACSF) excited control group and receptor agent NMDA group and AMPA group. Group 3 agonist group did not induce acute hypotension. Using micro pump at the rate of 1.5 l/min to the nucleus after.30min injection of drugs Open chest ventricular cavity blood serum after centrifugation, the content of serum norepinephrine by ELASA method. The T5-7 segment of the spinal cord by Western blotting analysis method (Western-Blot) was used to detect the expression of c-Fos in spinal cord and p-ERK1/2 protein. Results: 1. glutamic acid (Glu) ion receptor blocker (CNQX MK-801), after positioning needle injected into the medial vestibular nucleus (MVN) area, and sodium nitroprusside (SNP) induced acute hypotension, found expression in thoracic spinal cord of T5-7 segment of c-Fos protein and p-ERK1/2 protein content was significantly lower than the control group (ACSF) (P0.05).2. injection in the medial vestibular nucleus (Glu) glutamate ionotropic receptors agonist (NMDA, AMPA), the control group the expression content of thoracic spinal cord of T5-7 segment of c-Fos and p-ERK1/2 protein was significantly higher than that of the injection of artificial cerebrospinal fluid (P0.05) to.3. (Glu) two glutamate ion receptor blocker (CNQX, MK-801) was injected into the vestibule The medial nucleus (MVN) area, and sodium nitroprusside (SNP) acute hypotension induced by norepinephrine, found in serum (NE) were significantly lower than the control group (ACSF) (P0.05) glutamate.4. medial vestibular nucleus (Glu) of the ionotropic agonists (NMDA, AMPA), significantly increased norepinephrine the content of serum (NE), and the control group (ACSF) and there was significant difference (P0.05). Conclusion: acute hypotension induced by MVN can make the ionotropic glutamate receptor stimulation through regulating region of sympathetic nerve involved in blood pressure.

【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R544.2

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