Toll样受体-4基因启动子区多态性与急性心肌梗死发病风险的关系

发布时间：2018-03-05 03:13

本文选题：急性心肌梗死　切入点：Toll样受体-　出处：《山东医药》2017年17期 　论文类型：期刊论文

【摘要】：目的探讨Toll样受体-4(TLR4)基因多态性与急性心肌梗死(AMI)发病风险的关系。方法选取AMI患者244例(AMI组)和非AMI患者284例(对照组),采用聚合酶链反应-限制性片段长度多态性技术检测两组TLR4基因启动子区多态性(rs10116253和rs10983755),比较两组TLR4启动子区多态性的基因型分布差异,采用多因素Logistic回归模型分析TLR4基因启动子区多态性与AMI发病风险的关系。结果TLR4基因rs10116253、rs10983755均符合Hardy-Weiberg平衡。TLR4启动子区rs10116253和rs10983755多态性与AMI的总体发病风险无关(P均0.05)。对于TLR4基因rs10116253多态位点,男性TC突变杂合型携带者AMI的发病风险是TT野生纯合型携带者的0.50倍,TC+CC突变型携带者AMI发病风险是TT野生纯合型携带者的0.51倍,伴有高血脂的TC杂合型携带者AMI发病风险是TT野生纯合型携带者的0.42倍,TC+CC突变型携带者AMI发病风险是TT野生纯合型携带者的0.46倍,差异均有统计学意义(P均0.05)。TLR4基因rs10983755多态位点在性别、年龄、有无高血压、有无糖尿病及有无血脂异常等分层分析中均未见统计学差异(P均0.05)。结论TLR4基因启动子区rs10116253多态性与AMI分层发病风险存在关系;rs10116253突变基因型可以降低男性人群、伴有高血脂人群AMI的发病风险。
[Abstract]:Objective to investigate the relationship between the polymorphism of Toll like receptor -4 (TLR4) gene and the risk of acute myocardial infarction (AMI). Methods 244 AMI patients and 284 non-AMI patients (control group) were studied by polymerase chain reaction-restriction fragment length (PCR). The polymorphism of TLR4 gene promoter region rs10116253 and rs10983755 was detected by polymorphic technique, and the genotype distribution of TLR4 promoter polymorphism was compared between the two groups. Multivariate Logistic regression model was used to analyze the relationship between the polymorphism of TLR4 gene promoter region and the risk of AMI. Results TLR4 gene rs10116253 rs10983755 all accord with Hardy-Weiberg balance. Rs10116253 and rs10983755 polymorphism of TLR4 promoter region were not correlated with the overall risk of AMI (P < 0.05). At the rs10116253 polymorphic site of TLR4 gene, The risk of AMI in male carriers with TC mutation was 0.50 times as high as that of carriers with TT wild homozygote. The risk of AMI in carriers with TC CC mutation was 0.51 times higher than that of carriers with wild heterozygous type of TT. The risk of AMI in TC heterozygous carriers with hyperlipidemia was 0.42 times higher than that in TT wild homozygous carriers. The AMI risk of TC CC mutant carriers was 0.46 times higher than that of TT wild homozygous carriers. The differences were statistically significant (P < 0.05). The rs10983755 polymorphism of TLR4 gene was in sex, age, hypertension or not. There was no statistical difference in the stratified analysis of diabetes mellitus and dyslipidemia (P < 0.05). Conclusion there is a relationship between the rs10116253 polymorphism of TLR4 gene promoter region and the risk of AMI stratification. The mutation genotype rs10116253 can reduce the male population. Risk of AMI associated with hyperlipidemia.
【作者单位】：中国医科大学附属第一医院;
【基金】：辽宁省自然科学基金计划项目(2015020506)
【分类号】：R542.22

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