心脏骤停小鼠心肺复苏术后行为学和形态学改变

发布时间：2018-03-09 09:21

本文选题：心脏骤停　切入点：心肺复苏　出处：《临床心血管病杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的:探讨小鼠心脏骤停心肺复苏术后行为学和形态学的改变。方法:采用静脉注射氯化钾诱导心脏骤停的方法建立心脏骤停/心肺复苏(CA/CPR)小鼠模型。通过旷场实验观察小鼠行为学改变。制备脑片行HE染色,在光学显微镜及超微电镜下观察脑细胞形态的改变及细胞内超微机构的改变。结果:心肺复苏后野生型(WT:C3H/HeN)和突变型(TLR4-/-:C3H/HeJ)模型组小鼠周边区活动时间增加,中央区活动时间和路程显著减少,且WT模型组在中央区的活动时间较TLR4-/-模型组显著减少。光镜下发现TLR4-/-模型组脑细胞较WT模型组水肿、变性坏死较轻。超微电镜下发现海马及皮质脑细胞损伤最明显,线粒体、内质网和高尔基体等重要细胞器损伤,突触结构模糊,血管周围水肿。结论:从行为学方面来讲,心肺复苏后WT和TLR4-/-模型组小鼠趋避性及焦虑水平较高,且WT模型组较TLR4-/-模型组小鼠趋避性及焦虑水平更高。从形态学方面来讲,心肺复苏后TLR4-/-模型组脑细胞损伤较WT模型组轻。心脏骤停/心肺复苏后,TLR4-/-小鼠脑组织损伤程度均明显轻于WT小鼠,表明Toll样受体4在心肺复苏后脑细胞损伤中起重要作用。
[Abstract]:Objective: to investigate the changes of behavior and morphology after cardiopulmonary resuscitation in mice with cardiac arrest. Methods: the cardiac arrest / cardiopulmonary resuscitation model was established by intravenous injection of potassium chloride to induce cardiac arrest in mice. The behavioral changes of mice were observed. Brain slices were prepared for HE staining. The morphological changes of brain cells and the changes of intracellular ultrastructure were observed under optical microscope and ultramicroscope. Results: after cardiopulmonary resuscitation (CPR), the activity time of peripheral regions in the wild type WT: C3H / HeNand mutant TLR4-r-7C3H / HeJgroup increased, and the changes in the peripheral regions of the mice were also observed after cardiopulmonary resuscitation (CPR). The activity time and distance of the central region in WT model group were significantly decreased than that in TLR4-r-model group. Under light microscope, brain cells in TLR4-r-model group were more edema than those in WT model group. Degeneration and necrosis were mild. Ultrastructural damage of hippocampal and cortical brain cells was most obvious, mitochondria, endoplasmic reticulum and Golgi apparatus were damaged, synaptic structure was blurred, perivascular edema was observed. Conclusion: from the behavioral point of view, After cardiopulmonary resuscitation (CPR), WT and TLR4-r- model group had higher level of avoidance and anxiety, and WT model group had higher level of avoidance and anxiety than TLR4-r-model group. The brain cell damage in TLR4-r-model group after cardiopulmonary resuscitation was lighter than that in WT model group, and the degree of brain tissue injury in TLR4-r-r-mice after cardiac arrest / cardiopulmonary resuscitation was significantly lighter than that in WT mice, suggesting that Toll like receptor 4 plays an important role in brain cell injury after CPR.
【作者单位】：华中科技大学同济医学院附属协和医院急诊医学科;华中科技大学同济医学院附属协和医院超声科;华中科技大学同济医学院附属协和医院心内科;
【基金】：国家自然科学基金项目(No:811011401)
【分类号】：R541.78

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