转化生长因子β1基因沉默脂肪干细胞移植治疗血小板减少性紫癜

发布时间：2018-03-10 01:20

本文选题：干细胞　切入点：组织工程　出处：《中国组织工程研究》2017年01期 　论文类型：期刊论文

【摘要】：背景:有研究表明干细胞治疗为血小板减少性紫癜的治疗带来了新的希望,但对于此病的完美的治疗仍存在困难。目的:探讨转化生长因子β1基因沉默脂肪干细胞移植对血小板减少紫癜小鼠疗效的影响。方法:将构建的转化生长因子β1-siRNA质粒转染至小鼠脂肪干细胞中,使其低表达转化生长因子β1。用豚鼠抗小鼠血小板血清(GP-APS)腹腔注射构建血小板减少性紫癜模型小鼠,建模后随机分为4组,血小板减少性紫癜模型组尾静脉注射生理盐水20μL,脂肪干细胞组和转化生长因子β1沉默组分别尾静脉注射2×106 L~(-1)的CM-Dil标记的脂肪干细胞,转化生长因子β1沉默脂肪干细胞悬液20μL,泼尼松组按泼尼松5 mg/kg灌胃,连续治疗14 d。结果与结论:(1)免疫荧光染色及EILSA检测:与其他3组比较,转化生长因子β1沉默组CM-Dil标记的阳性细胞数目,血小板计数,产板型巨核细胞比例均升高,骨髓巨核细胞总数、PAIgG及转化生长因子β1水平下降(P0.05);(2)结果说明,转化生长因子β1基因沉默脂肪干细胞移植能有效降低血小板减少性紫癜小鼠转化生长因子β1及PAIgG水平,促进骨髓巨核细胞分化成熟,从而有效提高血小板计数,对治疗血小板减少性紫癜有效果较好。
[Abstract]:Background: some studies have shown that stem cell therapy has brought new hope for the treatment of thrombocytopenic purpura. But it is still difficult to perfect the treatment of this disease. Objective: to investigate the effect of transforming growth factor 尾 1 gene silencing adipose stem cell transplantation on the therapeutic effect of thrombocytopenic purpura mice. Methods: transforming growth factor 尾 1-siRNA was constructed in mice with thrombocytopenic purpura. The plasmid was transfected into the adipose stem cells of mice. The mice with thrombocytopenic purpura were established by intraperitoneal injection of GP-APS. The mice were randomly divided into 4 groups after modeling. In the thrombocytopenic purpura model group, 20 渭 L saline was injected into the tail vein, and the adipose stem cells group and the transforming growth factor 尾 1 silencing group were injected with CM-Dil labeled adipose stem cells respectively. Transforming growth factor 尾 1 silenced adipose stem cell suspension (20 渭 L). Prednisone group was treated with prednisone for 5 mg/kg for 14 days. Results and conclusion Immunofluorescence staining and EILSA detection: compared with the other three groups. In TGF- 尾 1 silencing group, the number of CM-Dil labeled positive cells, the platelet count, the percentage of platelet-producing megakaryocytes increased, and the total number of bone marrow megakaryocytes (PAIgG) and the level of transforming growth factor 尾 1 (TGF- 尾 1) decreased (P 0.05). Transforming growth factor 尾 1 gene silencing adipose stem cell transplantation can effectively reduce the levels of transforming growth factor 尾 1 and PAIgG in mice with thrombocytopenic purpura, promote the differentiation and maturation of bone marrow megakaryocyte, thus effectively increase the platelet count. It has good effect on treatment of thrombocytopenic purpura.
【作者单位】：河北医科大学第一医院血液科;
【基金】：河北省卫生厅医学科学研究课题(20130276)~~
【分类号】：R554.6

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