血管生成因子对心脏微血管内皮细胞神经调节蛋白1表达和分泌的影响

发布时间：2018-03-10 16:05

本文选题：血管内皮生长因子类　切入点：内皮细胞　出处：《中华老年心脑血管病杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的探讨血管内皮生长因子(VEGF)、血管形成蛋白1(Ang-1)和Ang-2对人源性心脏微血管内皮细胞(HCMEC)的神经调节蛋白1(NRG-1)表达和NRG-1β分泌的影响。方法培养HCMEC至4~5代,在正常培养条件下,将其分为正常组、VEGF处理组、Ang-1处理组和Ang-2处理组,与HCMEC共孵育24h,收集细胞和培养液,用蛋白印迹法和细胞酶联免疫吸附法分别检测NRG-1蛋白表达和NRG-1β分泌。蛋白印迹法检测酪氨酸激酶受体(ErbB2、ErbB3和ErbB4)蛋白表达。结果ErbB2、ErbB3和ErbB4均表达于HCMEC。与正常组比较,VEGF处理组和Ang-1处理组NRG-1[(1.44±0.05)、(1.18±0.04)vs(1.05±0.06)]表达和NRG-1β[(534.07±6.89)ng/L、(505.19±22.82)ng/L vs(380.71±14.96)ng/L]分泌显著增加,差异有统计学意义(P0.05),而Ang-2处理组NRG-1表达和NRG-1β分泌显著降低(P0.05)。结论VEGF和Ang-1显著增加HCMEC的NRG-1表达和NRG-1β分泌,Ang-2显著减少HCMEC的NRG-1表达和NRG-1β分泌。
[Abstract]:Objective to investigate the effects of vascular endothelial growth factor (VEGF), vascular morphogenetic protein 1 (Ang-1) and Ang-2 on the expression of neuroregulatory protein 1 (NRG-1) and the secretion of NRG-1 尾 in human cardiac microvascular endothelial cells (HCMECs). They were divided into normal group and Ang-2 treatment group. They were incubated with HCMEC for 24 hours to collect cells and culture medium. NRG-1 protein expression and NRG-1 尾 secretion were detected by Western blotting and enzyme-linked immunosorbent assay (Elisa), respectively. ErbB2ErbB3 and ErbB4) were detected by Western blot. Results ErbB2ErbB3 and ErbB4 were detected in HCMECs. The expression of NRG-1 (1.44 卤0.05) and the secretion of NRG-1 尾 (534.07 卤6.89 ng / L, 505.19 卤22.82 ng / L vs(380.71 卤14.96 ng / L) were significantly increased in the two groups (1.18 卤0.04 卤0.04 卤1.05 卤0.06). The expression of NRG-1 and the secretion of NRG-1 尾 in Ang-2 group were significantly lower than those in control group. Conclusion VEGF and Ang-1 can significantly increase NRG-1 expression of HCMEC and decrease NRG-1 expression and NRG-1 尾 secretion of HCMEC by NRG-1 尾 -Ang-2.
【作者单位】：广西医科大学第一附属医院心内科;
【基金】：国家自然科学基金(81160021,81160042,81160046) 广西自然科学基金重点课题(2014GXNSFDA118024)
【分类号】：R54

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