阿霉素诱导的扩张型心肌病大鼠心律失常与内质网应激相关性研究

发布时间：2018-03-11 21:36

本文选题：扩张型心肌病　切入点：心律失常　出处：《临床心血管病杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的:研究阿霉素诱导的扩张型心肌病大鼠心律失常与内质网应激的关系。方法:30只Wister雄性大鼠分为对照组、阿霉素模型组(模型组),每组15只,对照组腹腔注射0.9%Nacl(10ml/kg),共3次(每周1次);模型组腹腔注射阿霉素2mg/kg,共注射3次(每周1次)。7周末2组大鼠行心电图检测心律失常发生率及心脏彩超检测左室舒张末径及左心室射血分数,2组大鼠处死后取左心室组织行苏木精-伊红、masson、VG染色,观察心肌病理改变,采用real time PCR及Western blotting技术检测2组大鼠心肌细胞内质网应激伴侣蛋白GRP78,GRP94表达。结果:与对照组大鼠比较,模型组大鼠心律失常发生率增多(P0.05),心肌细胞内质网应激伴侣蛋白GRP78,GRP94表达明显增多(P0.01)。结论:阿霉素诱导扩张型心肌病大鼠心律失常发生可能与心肌细胞内质网应激相关。
[Abstract]:Objective: to study the relationship between arrhythmia and endoplasmic reticulum stress in dilated cardiomyopathy rats induced by doxorubicin. Methods: thirty male Wister rats were divided into control group and adriamycin model group (model group, 15 rats in each group). The control group was injected with 0.9 Naclum 10 ml / kg for 3 times (once a week) and the model group was injected with adriamycin (2 mg / kg) for 3 times (once a week, 7 weeks a week). The incidence of arrhythmia was detected by electrocardiogram and the left ventricular end diastolic value was detected by echocardiography. The left ventricular tissue was taken from the rats in the diameter and left ventricular ejection fraction group after death for hematoxylin-eosinolone VG staining. The expression of ER stress chaperone protein GRP78 and GRP94 was detected by real time PCR and Western blotting techniques. In the model group, the incidence of arrhythmia increased and the expression of ER stress chaperone protein GRP78 / GRP94 in cardiac myocytes increased significantly. Conclusion: the incidence of arrhythmia in rats with dilated cardiomyopathy induced by adriamycin may be related to the endoplasmic reticulum stress in cardiomyocytes.
【作者单位】：内蒙古民族大学医学院;内蒙古民族大学附属医院;
【基金】：国家自然科学基金项目(No:81360587) 内蒙古民族大学科学研究项目(No:NMDYB1733)
【分类号】：R541.7;R542.2

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