SIRT1基因多态性与高血压性脑出血的相关性研究

发布时间：2018-03-12 15:34

本文选题：SIRT1　切入点：高血压性脑出血　出处：《福建中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨SIRT1 rs2273773、rs7069102、rs7895833基因多态性与高血压性脑出血的相关性,为该病的预防和康复预后提供临床基础。方法:本研究采用病例-对照研究方法,收集201例高血压性脑出血患者及203例种族、年龄、性别匹配的对照组为研究对象,用聚合酶链反应-限制性片段长度多态性方法分析SIRT1基因rs2273773、rs7069102、rs7895833多态性,我们测定了入院时血压值以及外周血FBG、TG、TC、HDLC、LDLC等,对高血压性脑出血患者进行NIHSS量表评定,使用SPSS20.0软件进行统计分析,比较SIRT1基因rs2273773、rs7069102、rs7895833多态性在病例组和对照组之间的分布情况,同时比较不同基因型间血压、空腹血糖及生化指标水平的差异,在高血压性脑出血组中比较不同基因型间NIHSS评分水平的差异。结果:1、高血压性脑出血组(Hypertensive Cerebral Hemorrhage,HICH)与对照组间年龄、性别无显著性差异(P0.05),但吸烟史、饮酒史、糖尿病史、FBG在高血压性脑出血组明显高于对照组(P0.01);TC、HDLC在高血压性脑出血组明显低于对照组(P0.05)。2、SIRT1基因rs2273773、rs7069102多态性基因型频率及等位基因频率在高血压性脑出血组与对照组间无显著差异(P0.05),而在高血压性脑出血患者组中SIRT1基因rs7895833多态性AA基因型及A等位基因频率均显著高于对照组(P0.05)。非条件logistic回归分析显示,以GG基因型为对照,高血压性脑出血组的AA基因型发病风险升高有关(P=0.008,OR=3.360,95%CI:1.370-8.239),另外以G等位基因为对照,高血压性脑出血组携带A等位基因的发病风险升高(P=0.048,OR=1.369,95%CI:1.003-1.869)。3、SIRT1 基因 rs2273773、rs7069102 及 rs7895833 位点各基因型与血压、FBG、生化指标进行分析,发现rs7895833位点在SBP水平呈AAAGGG趋势,rs2273773位点在HDLC水平呈CCCTTT趋势,差异均有统计学意义(P0.05)。在高血压性脑出血组中rs2273773、rs7069102及rs7895833位点各基因型与NIHSS得分进行对比无统计学差异(P0.05)。4、多因素logistic回归分析高血压性脑出血的相关危险因素表明,甘油三脂、低密度脂蛋白、SBP、rs7895833多态性是高血压性脑出血的独立危险因素(p0.05),携带rs7895833AA型和A等位基因的患者罹患高血压性脑出血的发病率高,而高密度脂蛋白为高血压脑出血组的独立保护因素(P0.05)。结论:1、SIRT1基因可能是福建汉族人群高血压性脑出血的的遗传易感基因,其rs7895833位点AA基因型和等位基因A携带者增加高血压性脑出血的发病风险,但尚需扩大样本量进一步证实。2、SIRT1基因rs7895833多态性与收缩压水平相关,rs2273773多态性与HDLC水平有关,而SIRT1基因rs2273773、rs7069102及rs7895833多态性与高血压性脑出血组功能障碍程度(NIHSS评分)可能无关。
[Abstract]:Objective: To investigate the SIRT1 rs2273773, rs7069102, rs7895833 gene polymorphism in patients with hypertensive cerebral hemorrhage, and provide clinical basis for the prevention and rehabilitation and prognosis of the disease. Methods: This study used a case-control study, collected 201 cases of patients with hypertensive cerebral hemorrhage and 203 cases of race, age, sex matched as control group the object of study, using polymerase chain reaction restriction fragment length polymorphism analysis of SIRT1 gene rs2273773, rs7069102, rs7895833 polymorphism, we measured blood pressure and peripheral blood FBG, TG on admission, TC, HDLC, LDLC, NIHSS were assessed in patients with hypertensive cerebral hemorrhage, was used for statistical analysis the software of SPSS20.0, SIRT1 genes rs2273773, rs7069102, the distribution of rs7895833 polymorphism between the patient group and control group, and the comparison between different genotypes and biochemical indexes of blood pressure, fasting blood sugar water Flat differences, differences in the level of NIHSS score in group comparison among different genotypes of hypertensive cerebral hemorrhage. Results: 1 hypertensive cerebral hemorrhage group (Hypertensive Cerebral, Hemorrhage, HICH) and the control group had no significant difference between age, gender (P0.05), but the smoking history, drinking history, history of diabetes, FBG in hypertensive cerebral hemorrhage group was significantly higher than the control group (P0.01); TC, HDLC in hypertensive cerebral hemorrhage group was significantly lower than the control group (P0.05.2), SIRT1 gene rs2273773, rs7069102 polymorphism genotypes and allele frequencies in hypertensive cerebral hemorrhage had no significant difference between the group and the control group (P0.05). In SIRT1 group and rs7895833 gene polymorphism of AA genotype and A allele frequency of hypertensive cerebral hemorrhage were significantly higher than control group (P0.05). Non conditional logistic regression analysis showed that GG genotype on hypertensive cerebral hemorrhage group, AA gene The increased risk of type (P=0.008, OR=3.360,95%CI:1.370-8.239), in addition to the G allele were increased the risk of hypertensive cerebral hemorrhage group carrying the A allele (P=0.048, OR=1.369,95%CI:1.003-1.869).3, SIRT1 rs2273773 and rs7069102 gene, rs7895833 locus genotypes and FBG analysis, blood pressure, biochemical index, rs7895833 sites were AAAGGG trend in the level of SBP, rs2273773 loci showed CCCTTT trend in the HDLC level, the differences were statistically significant (P0.05). In hypertensive cerebral hemorrhage group in rs2273773, rs7069102 and rs7895833 loci genotypes and NIHSS score were compared no significant difference (P0.05.4), logistic regression analysis of related risk of cerebral hemorrhage the factors of hypertension showed that glycerin three fat, low density lipoprotein, SBP, rs7895833 polymorphism is an independent risk factor of hypertension cerebral hemorrhage (P0.05), Carrying the rs7895833AA genotype and A allele in patients with hypertension cerebral hemorrhage rate is high, and high density lipoprotein is an independent protective factor for hypertension cerebral hemorrhage group (P0.05). Conclusion: 1. The SIRT1 gene may be a susceptible gene in genetic hypertensive cerebral hemorrhage in Fujian Han population, the rs7895833 locus AA genotype and allele A carriers increased risk of hypertensive cerebral hemorrhage, but there is still a need to expand the sample size further confirmed that.2 and SIRT1 gene rs7895833 polymorphism and systolic blood pressure levels, rs2273773 polymorphism is related to the level of HDLC, SIRT1 and rs7895833 gene rs2273773, rs7069102 polymorphism and hypertensive cerebral hemorrhage group dysfunction (NIHSS score) may have nothing to do.

【学位授予单位】：福建中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R544.1;R743.34

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