Ginkgo biloba extract Monocrotaline Pulmonary arterial hyper

  本文关键词：银杏叶提取物对野百合碱所致肺动脉高压保护作用的实验研究，，由笔耕文化传播整理发布。

银杏叶提取物对野百合碱所致肺动脉高压保护作用的实验研究

Protective effect of ginkgo biloba extract on monocrotaline-induced pulmonary hypertension

[1] [2] [3] [4] [5] [6] [7]

Shen Tao, Wu Xu, Zhang Zhenzhong, Gu Chengwei, Li Jun, Wu Xia, Gao Dongyun （Department of Thoracic and Cardiovascular Surgery, Nanfang Hospital, Southern Medical University, G

南方医科大学附属南方医院胸心血管外科,广东广州510515

文章摘要：目的：探讨银杏叶提取物对野百合碱所致肺动脉高压（PAH）的早期保护作用。方法：30只雄性 SD大鼠随机分为3组：对照组、PAH 模型组和治疗组,每组10只。PAH 模型组和治疗组大鼠采用脊背部皮下注射1%野百合碱60 mg/kg的方法复制PAH 模型,注射后第2天开始每日予2 ml 0.9%氯化钠注射液（PAH 模型组）或60 mg/kg银杏叶提取物（治疗组）灌胃；对照组脊背部皮下注射等量溶剂,注射后第2天开始每日予2 ml 0.9%氯化钠注射液灌胃。第22天采用颈外静脉右心导管法测定平均肺动脉压（mPAP）、右心室收缩压（RVSP）,测定右心室、左心室、室间隔的重量,计算右心室肥厚指数（RVHI）；采用 HE染色观察肺细小动脉组织结构情况,并计算直径50~150μm的肺小动脉血管管壁厚度占血管厚度百分比（WA%）和血管管壁面积与血管面积比值（WV%）；采用免疫组化方法观察内皮型一氧化氮合成酶（eNOS）及内皮素-1（ET-1）在大鼠肺组织中的表达情况。结果：PAH 模型组、治疗组的 mPAP、RVSP、RVHI、WA%、WV%与对照组比较均明显升高（P〈0.05）,治疗组的 mPAP、RVSP、WA%、WV%较 PAH 模型组均降低（P〈0.05）,而治疗组与 PAH 模型组的RVHI差异无显著性。免疫组化结果显示,3组 eNOS表达,对照组〉治疗组〉PAH 模型组；3组 ET-1的表达, PAH 模型组〉治疗组〉对照组。结论：银杏叶提取物通过抑制 ET-1的合成、减轻内皮细胞损伤、维持 eNOS的表达,达到减缓PAH 进展的作用。

Abstr：Objective：To investigate the protective effect of ginkgo biloba extract （GBE）on monocrotaline-in-duced pulmonary hypertension in early stage.Methods：Thirty male SD rats were randomly divided into three groups ：control group,pulmonary hypertension model group （model group）,ginkgo biloba extract treatment group （treatment group,n=10）. The pulmonary hypertension model was reproduced by subcutaneous injection of monocrotaline （60 mg/kg）on the back,and then,2 ml normal saline or 60 mg/kg GBE was given by gavage from the second day in model group or treatment group. In control group,the same volume of vehicle was subcutane-ously inj ected instead of monocrotaline,and 2 ml normal saline was given by gavage from the second day. Mean pulmonary arterial pressure （mPAP）and right ventricular systolic pressure （RVSP）were measured through cath-eterization into the right ventricle via the right jugular vein on the 22nd day. The right ventricular,left ventricle, and interventricular septum were weighed to calculate the right ventricular hypertrophic index （RVHI）.The structure of pulmonary arterioles was observed with HE staining. The ratio of wall thickness/diameter of arteri-oles （WA%）,and the ratio of vascular wall area/vascular cross-sectional area （WV%）of pulmonary arterioles with diameter of 50-150μm were calculated. The expression of endothelial nitric oxide synthase （eNOS）and en-dothelin-1 （ET-1）in the lung tissue of rats were assayed by immunohistochemical method.Results：Compared to the control group,mPAP,RVSP,RVHI,WA% and WV% were significantly increased in model group and treatment group （P〈0.05）.In the treatment group,mPAP,RVSP,WA% and WV% were lower than those of the model group （P〈0.05）,but no significant change was found in RVHI. Immunoh

文章关键词：

Keyword：:Ginkgo biloba extract Monocrotaline Pulmonary arterial hypertension Endothelial nitric oxide synthase Endothelin-1

课题项目：广东省科技计划项目（20088010600006）

作者信息：会员可见

  本文关键词：银杏叶提取物对野百合碱所致肺动脉高压保护作用的实验研究，由笔耕文化传播整理发布。