靶向PPARγ的miRNA在冠脉支架内再狭窄中的表达及意义

发布时间：2018-03-14 10:53

本文选题：冠心病　切入点：支架内再狭窄　出处：《中国动脉硬化杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的探索靶向于过氧化体增殖物激活型受体γ(PPARγ)的miRNA,在这些miRNA中寻找与支架内再狭窄(ISR)密切相关的miRNA,并初步探索其临床意义。方法利用Cytoscape及其插件构建miRNA与PPARγ的作用网络,筛选出靶向PPARγ的关键miRNA。利用实时荧光定量聚合酶链反应(Real-time PCR)检测这部分miRNA在ISR人群中的表达差异,再通过ROC曲线评估miRNA对ISR患者的临床识别能力。结果通过生物信息学分析发现,miR-27a/b对PPARγ的调控作用较强;而miR-130a/b则能调控该网络中更多的基因。Real-time PCR发现miR-27a/b和miR-130a在ISR组较non-ISR组存在差异性表达(P0.05)。ROC曲线分析发现,miR-27a对ISR具有一定的临床识别能力(AUC=0.884,95%CI 0.768~1.00,P0.001)。结论 miR-27a/b和miR-130a/b是靶向于PPARγ的主要miRNA,miR-27a/b和miR-130a在ISR中差异性表达,miR-27a可能对识别ISR患者具有一定的临床意义。
[Abstract]:Objective to explore the miRNAs targeting peroxisome proliferator activated receptor 纬 (PPAR- 纬), and to explore the clinical significance of these miRNAs closely related to restenosis in stents. Methods Cytoscape and its plug-ins were used to construct the network of miRNA and PPAR 纬. The key PPAR 纬 targeted miRNAs were screened. Real-time PCR was used to detect the difference of miRNA expression in ISR population. The clinical recognition ability of miRNA to ISR patients was evaluated by ROC curve. Results through bioinformatics analysis, it was found that miR-27a / b had a strong regulatory effect on PPAR 纬. While miR-130a/b could regulate more genes in the network. Real-time PCR found that miR-27a/b and miR-130a expressed differently in ISR group than in non-ISR group. Analysis of P0.05. ROC curve showed that miR-27a had certain clinical recognition ability to ISR. Conclusion miR-27a/b and miR-130a/b are targeted at PPAR 纬. The differential expression of miR-27a / b and miR-130a in ISR may be of clinical significance in the recognition of ISR patients.
【作者单位】：济宁医学院附属医院心内科;济宁医学院附属医院山东省心脏疾病诊疗重点实验室;济宁医学院附属医院心外科;
【基金】：山东省医药卫生科技发展项目(2014WS0515) 济宁医学院重点项目(JY2013KJ005)
【分类号】：R541.4

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