下丘脑室旁核CRH神经元激活在慢性充血性心力衰竭中的交感兴奋作用

发布时间：2018-03-18 11:07

本文选题：慢性充血性心力衰竭　切入点：下丘脑室旁核　出处：《中国病理生理杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的:观察慢性心衰时下丘脑室旁核(PVN)内促肾上腺皮质激素释放激素(CRH)表达变化及其与交感神经活动之间的关系。方法:健康雄性SD大鼠,冠脉结扎制备心衰模型,侧脑室插管渗透压泵持续给药。假手术组和心衰组给予人工脑脊液0.25μL/h,心衰给药组给予CRH抑制剂αh-CRH 15 mg/h。同时,选取健康雄性体内CRH合成不足的Lewis大鼠与同源纯种Fischer 344大鼠分别制备心衰模型和假手术对照进行对比研究。4周后,测定左室舒张末压(LVEDP)、左室内压最大上升和下降速率(±dp/dtmax)、右心室/体重比(RV/BW)、肺/体重比(lung/BW)、肾交感神经放电活动(RSNA)、血浆去甲肾上腺素(NE)浓度和PVN内CRH阳性神经元数目。血浆促肾上腺皮质激素(ACTH)含量。结果:与假手术组相比,SD心衰大鼠PVN内CRH阳性神经元数目明显增加,血浆ACTH浓度升高,RSNA增强,血浆NE浓度增加,LVEDP、lung/BW和RV/BW增加,±dp/dtmax降低;心衰模型后给予αh-CRH可明显逆转上述各种变化(P0.05)。Fisher 344大鼠心衰组和假手术对照相比,PVN内CRH阳性神经元数目明显增加,血浆ACTH浓度升高,RSNA增强,外周血NE浓度升高,LVEDP、RV/BW和lung/BW增加,±dp/dtmax下降(P0.05)。但Lewis大鼠心衰组和假手术对照相比,以上各指标改变均不明显。结论:慢性心衰时,下丘脑室旁核CRH神经元被激活,激活的CRH神经元可增强外周交感神经活动,加重心功能恶化。
[Abstract]:Objective: to observe the expression of corticotropin releasing hormone (CRH) in PVN of hypothalamic paraventricular nucleus (PVN) and its relationship with sympathetic nerve activity in chronic heart failure (CHF). The sham operation group and heart failure group were given artificial cerebrospinal fluid 0.25 渭 L / h, and the heart failure group was given CRH inhibitor 伪 h-CRH 15 mg / h. Healthy male Lewis rats with insufficient CRH synthesis and homologous pure Fischer 344 rats were selected to establish heart failure model and sham operation control for 4 weeks. Left ventricular end-diastolic pressure (LVEDPN), left ventricular pressure maximum rise and fall rate (卤DP / dtmaxg), right ventricular / body weight ratio (RV / BW), lung / body weight ratio (RV / BW), renal sympathetic nerve activity (RSNA), plasma norepinephrine (NE) and the number of CRH positive neurons in PVN were measured. Results: compared with sham operation group, the number of CRH positive neurons in PVN of SD heart failure rats was significantly increased. The increase of plasma ACTH concentration, the increase of plasma NE concentration and the increase of LVEDPDPlung / BW and RV/BW, and the decrease of 卤dp/dtmax, 伪 h-CRH could reverse these changes significantly after heart failure model and the number of CRH positive neurons in heart failure group was significantly higher than that in sham operation group. The increase of plasma ACTH concentration, the increase of NE concentration in peripheral blood, the increase of LVEDPDPV / BW and lung/BW, and the decrease of 卤dp/dtmax in RV / BW and lung/BW, but the changes of these indexes were not significant in Lewis rats compared with those in sham operation group. Conclusion: in chronic heart failure, there is no significant change in RV / BW and lung/BW in patients with chronic heart failure. The CRH neurons in the paraventricular nucleus of hypothalamus were activated, and the activated CRH neurons could enhance the peripheral sympathetic nerve activity and aggravate the deterioration of cardiac function.
【作者单位】：山西医科大学病理学教研室;山西医科大学转化医学研究中心;山西医科大学药理学教研室;西安交通大学医学院病理与病理生理学教研室;
【基金】：山西省自然科学基金资助项目(No.2014011043-6) 山西医科大学博士启动基金项目(No.03201101);山西医科大学基础医学院331基础医学科技培植基金项目(No.201412)
【分类号】：R541.61

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