中国2型糖尿病人群葡萄糖依赖性促胰岛素肽受体基因多态性与冠心病风险的相关性研究

发布时间：2018-03-21 07:28

本文选题：葡萄糖依赖性促胰岛素肽受体　切入点：单核苷酸多态性　出处：《中国糖尿病杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的观察中国汉族T2DM患者中葡萄糖依赖性促胰岛素肽(GIP)及其受体(葡萄糖依赖性促胰岛素肽受体,GIPR)基因多态性与冠心病风险的相关性。方法纳入666例T2DM患者,其中冠心病组390例,对照组276例。根据HapMapCHB数据库选取单体型标记的单核苷酸多态性(tagSNPs)。GIPR基因共4个tag-SNPs,分别为rs1800437、rs11671664、rs4803846和rs12709891。应用聚合酶链式反应-限制性内切酶片断长度多态性方法,对GIPR基因tag-SNPs进行等位基因和基因型频率分析,研究GIPR基因多态性与冠心病风险的关系。结果两组GIPR基因SNPs等位基因频率比较,差异无统计学意义。冠心病组和对照组rs1800437、rs11671664、rs4803846和rs12709891等位基因频率分别为79.8%vs 82.5%,62.6%vs 59.3%,89.0%vs 89.3%和48.8%vs 50.4%。两组GIPR基因rs1800437和rs4803846单体型组合比较,差异无统计学意义。冠心病组和对照组单体型组合GG、GC和AG频率分别为69.2%vs 72.0%,19.8%vs 17.3%和10.6%vs 10.5%。结论中国T2DM患者中,未发现GIPR基因多态性与冠心病发生风险相关的SNPs,提示GIP受体基因变异可能不是中国T2DM患者冠心病风险的主要决定因素。
[Abstract]:Objective to investigate the association between GIPRR gene polymorphism and coronary heart disease risk in Chinese Han patients with T2DM. Methods 666 patients with T2DM were enrolled in this study. According to the HapMapCHB database, four tag-SNPs of tagSNPsU. GIPR gene were selected, which were rs1800437, rs11671664, rs4803846 and rs12709891.The polymerase chain reaction-restriction endonuclease fragment length polymorphism method was used. The allele and genotype frequency of GIPR gene tag-SNPs were analyzed to study the relationship between GIPR gene polymorphism and coronary heart disease risk. Results the frequency of GIPR gene SNPs allele was compared between the two groups. The allele frequencies of rs1800437, rs11671664, rs4803846 and rs12709891 in coronary heart disease group and control group were 79.8 vs 82.5 and 62.6, respectively. The frequencies of GIPR gene rs1800437 and rs4803846 haplotype were 89. 0 vs 89.3% and 48. 8%, respectively. The frequency of GC and AG of haplotype combination in coronary heart disease group and control group were 69.2 vs 72.0 and 19.8 vs 17.3% vs 10.6 vs 10.50.Conclusion in Chinese patients with T2DM, the frequency of GC and AG is 69.2% and 10.6%, respectively. No GIPR gene polymorphism was found to be associated with the risk of coronary heart disease, suggesting that GIP receptor gene variation may not be the main determinant of coronary heart disease risk in T2DM patients in China.
【作者单位】：北京大学第一医院内分泌科;
【基金】：国家自然科学基金(30771033)
【分类号】：R587.1;R541.4

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