不同类型冠心病患者Lp-PLA2基因N133K位点多态性的意义

发布时间：2018-03-22 14:40

  本文选题：脂蛋白相关磷脂酶A基因　切入点：基因多态性　出处：《广东医学》2017年04期 　论文类型：期刊论文

【摘要】：目的检测人脂蛋白相关磷脂酶A2(Lp-PLA2)基因N133K位点多态性与不同类型冠心病之间的相关性及临床意义。方法以心绞痛患者426例为心绞痛组、心肌梗死患者415例为心梗组,以同期体检健康者400例为对照组。应用聚合酶链反应(PCR)分析技术结合DNA直接测序技术,对入选者Lp-PLA2基因N133K(C/G)位点多态性进行分型。采用多因素logistic回归分析Lp-PLA2基因N133K多态位点与不同类型冠心病的相关性。同时检测入组者血脂及Lp-PLA2水平等指标。结果总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、Lp-PLA2水平在3组间差异有统计学意义(均P0.05),其中心梗组TC、TG、LDL-C、Lp-PLA2高于心绞痛组和对照组,HDL-C低于心绞痛组和对照组,心绞痛组TC、TG、LDL-C、Lp-PLA2高于对照组,HDL-C低于对照组(均P0.05)。心梗组和心绞痛组1个月后复查时,Lp-PLA2水平均明显降低(均P0.05)。心梗组GG、GC基因型以及G等位基因的分布频率明显高于心绞痛组和对照组,心绞痛组GG、GC基因型以及G等位基因的分布频率明显高于对照组(均P0.05)。Logistic回归分析发现,Lp-PLA2基因N133K(C/G)多态位点GG基因型与冠心病的发生有显著的相关性(P0.01)。结论Lp-PLA2基因N133K(C/G)多态位点基因突变可能与冠心病病情有关。
[Abstract]:Objective to investigate the relationship between N133K locus polymorphism of human lipoprotein associated phospholipase A _ 2 (Lp-PLA2) gene and different types of coronary heart disease (CHD). Methods 426 patients with angina pectoris and 415 patients with myocardial infarction were selected as patients with angina pectoris (angina pectoris) and 415 patients with myocardial infarction (MI). In the same period, 400 healthy persons were used as control group. Polymerase chain reaction (PCR) and DNA direct sequencing were used. The polymorphism of Lp-PLA2 gene N133K / C / G was genotyped. Multivariate logistic regression analysis was used to analyze the correlation between N133K polymorphism of Lp-PLA2 gene and different types of coronary heart disease. Blood lipid and Lp-PLA2 level were also detected. There were significant differences in the levels of Lp-PLA2 among the three groups (P < 0.05), among which the levels of LDL-CU Lp-PLA2 in the myocardial infarction group were higher than those in the angina pectoris group and the control group were lower than those in the angina pectoris group and the control group (P < 0.05), and the levels of Lp-PLA2 were significantly higher in the myocardial infarction group than in the angina pectoris group and the control group (P < 0.05), and the levels of Lp-PLA2 were significantly higher in the MI group than in the angina pectoris group and the control group. The level of Lp-PLA2 in angina group was significantly lower than that in control group (P 0.05). The level of Lp-PLA2 in MI group and angina pectoris group was significantly lower than that in angina group after 1 month (P 0.05). The frequency of GGG GC genotype and G allele in MI group was significantly higher than that in angina pectoris group and control group. The frequency of GC genotype and G allele in angina pectoris group was significantly higher than that in control group (all P0.05).Logistic regression analysis showed that there was a significant correlation between GG genotype of Lp-PLA2 gene N133KU C / G) and the occurrence of coronary heart disease. Conclusion the Lp-PLA2 gene N133KU CG / C / G is more than that of control group (P < 0.05). Conclusion there is a significant correlation between GG genotype of Lp-PLA2 gene and the occurrence of coronary heart disease. The mutation of the locus gene may be related to the condition of coronary heart disease.
【作者单位】： 河北省人民医院检验科;河北省人民医院功能科;河北省人民医院药学部;
【基金】：河北省科技计划项目(编号:16277771D)
【分类号】：R541.4;R440
，

本文编号：1649101