FTY720对糖尿病大鼠心肌微血管病变的保护作用

发布时间：2018-03-23 04:29

  本文选题：FTY　切入点：糖尿病　出处：《中国动脉硬化杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的研究1-磷酸鞘氨醇受体激动剂芬戈莫德(FTY720)对糖尿病大鼠早期心肌微血管病变的作用。方法将Spragure-Dawley大鼠分为正常对照组、糖尿病模型组与FTY720[0.001 g/(kg·d)]治疗组。大剂量链脲佐菌素(65 mg/kg)腹腔注射制备大鼠糖尿病模型,HE、Masson染色观察心肌结构大体形态。CD34免疫组织化学观察心肌微血管密度,TUNEL免疫荧光检测分析心脏微血管内皮细胞凋亡情况。组织转化生长因子β(TGF-β)、免疫组织化学观察心肌纤维化程度。Western blot测定血管细胞黏附分子1(VCAM-1)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)表达水平。结果糖尿病模型组以多次随机血糖≥16.7 mmol/L作为糖尿病模型成功的标志,本研究中大鼠糖尿病模型构建成功率在85%以上。与正常对照组相比,糖尿病模型组大鼠心肌组织微血管壁增厚,VCAM-1、TNF-α和IL-6的表达升高(P0.05);FTY720治疗组心肌VCAM-1、TNF-α与IL-6的表达明显低于糖尿病模型组(P0.05)。糖尿病模型组心脏组织微血管密度减少,凋亡指数显著增高;而FTY720可以减少凋亡,促进内皮细胞增殖。结论 FTY720可能在糖尿病大鼠心脏微血管损伤中具有一定保护作用。
[Abstract]:Objective to study the effect of 1-sphingosine phosphate receptor agonist, Fengomod FTY720, on early myocardial microangiopathy in diabetic rats. Methods Spragure-Dawley rats were divided into normal control group. Diabetic model group and FTY720 [0.001 g/(kg d] treatment group. High dose streptozotocin 65 mg / kg intraperitoneal injection was used to make diabetic model in rats. Observation of myocardial structure and morphology. CD34 immunohistochemical method was used to observe myocardial microvessel density and Tunel immunofluorescence. Detection and analysis of apoptosis in cardiac microvascular endothelial cells. Tissue transforming growth factor 尾 -TGF- 尾, immunohistochemical observation of myocardial fibrosis degree. Western blot detection of vascular cell adhesion molecule 1 VCAM-1, tumor necrosis factor 伪 TNF- 伪) and interleukin 6 IL-6) tables. Results multiple random blood glucose 鈮，

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