冠心病患者外周血NLRP3及相关因子表达的初步研究

发布时间：2018-03-24 03:00

本文选题：NLRP3　切入点：IL-1β　出处：《广西医科大学》2017年硕士论文

【摘要】：目的通过检测稳定性心绞痛、不稳定性心绞痛和急性心肌梗死患者外周血NLRP3及相关因子IL-1β、IL-18的水平差异,分析NLRP3及相关因子IL-1β、IL-18与C反应蛋白(CRP)、白细胞计数、左室射血分数(LVEF)的相关性,初步了解NLRP3及相关因子在冠心病患者中的表达水平,探讨NLRP3及相关因子与冠心病患者冠状动脉粥样斑块不稳定性的关系。方法选择于2015年3月到2015年8月在广西医科大学第四附属医院(柳州市工人医院)因胸痛住院的患者80例,根据冠心病诊断标准分型,其中急性心肌梗死(AMI组)患者26例,不稳定性心绞痛(UAP组)患者28例,稳定性心绞痛(SAP组)患者26例,所有患者均行冠状动脉造影(CAG)检查并明确诊断为冠心病,同期CAG正常的患者24例作为对照组(NC组)。用酶联免疫吸附法(ELISA)测定每组患者外周血中NLRP3、IL-1β、IL-18蛋白的浓度,并应用Pearson相关来分析NLRP3、IL-1β、IL-18和CRP、白细胞计数、LVEF之间的相关性。结果冠心病患者NLRP3、IL-1β外周血表达水平明显高于对照组,AMI组的NLRP3、IL-1β及IL-18蛋白水平均显著高于UAP组、SAP组和NC组(均P0.05),UAP组和SAP组的NLRP3及IL-1β蛋白水平明显高于NC组(均P0.05),UAP组的NLRP3蛋白水平明显高于SAP组(F=5.071,P=0.029),且冠心病患者血浆NLRP3、IL-1β及IL-18蛋白水平分别与白细胞计数及CRP呈正相关关系,与LVEF为负相关的关系。结论在冠心病患者中,AMI组及UAP组患者的外周血NLRP3蛋白浓度明显高于SAP组,提示NLRP3的高表达可能与冠心病患者冠状动脉粥样斑块的不稳定性相关,且冠心病患者外周血NLRP3、IL-1β及IL-18蛋白水平与LVEF均为负相关关系。
[Abstract]:Objective to detect the levels of NLRP3 and IL-1 尾 -IL-18 in peripheral blood of patients with stable angina pectoris, unstable angina pectoris and acute myocardial infarction. The correlation of left ventricular ejection fraction (LVEF) and the expression of NLRP3 and related factors in patients with coronary heart disease. To investigate the relationship between NLRP3 and related factors and the instability of coronary atherosclerotic plaque in coronary heart disease patients, 80 patients with chest pain were selected from March 2015 to August 2015 in the fourth affiliated Hospital of Guangxi Medical University (Liuzhou Workers Hospital). According to the diagnostic criteria of coronary heart disease, 26 patients with acute myocardial infarction (AMI), 28 patients with unstable angina pectoris (UAP), 26 patients with stable angina pectoris (SAP) and 26 patients with stable angina pectoris (SAP) were classified. All patients were examined by coronary angiography (CAG) and clearly diagnosed as coronary heart disease (CHD). 24 patients with normal CAG were used as control group. Elisa was used to detect the level of IL-1 尾 -IL-18 protein in peripheral blood of each group. Pearson correlation was used to analyze the correlation between IL-18, CRP, WBC count and IL-18. Results the levels of IL-1 尾 and IL-18 in peripheral blood of patients with coronary heart disease were significantly higher than those of control group (P < 0.05). The levels of NLRP3 and IL-1 尾 protein in both P0.05 and SAP groups were significantly higher than those in NC group (both P0.05 / UAP group and SAP group were significantly higher than that in SAP group. There was a positive correlation between the plasma levels of NLRP3P IL-1 尾 and IL-18 protein and leukocyte count and CRP in patients with coronary heart disease. Conclusion the concentration of NLRP3 protein in peripheral blood of patients with coronary heart disease is significantly higher than that of SAP group, suggesting that the high expression of NLRP3 may be related to the instability of coronary atherosclerotic plaque in patients with coronary heart disease. The levels of IL 1 尾 and IL-18 protein in peripheral blood of patients with coronary heart disease were negatively correlated with LVEF.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.4

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