护骨素信号介导Ghrelin调控A7r5主动脉平滑肌细胞钙化

发布时间：2018-04-01 15:33

本文选题：胃促生长素　切入点：护骨素　出处：《中国动脉硬化杂志》2017年11期

【摘要】：目的探讨胃促生长素(Ghrelin)与护骨素(OPG)/细胞核因子κB受体活化因子配体(RANKL)在钙化发生过程中的调控机制。方法首先观察高脂高糖培养基中A7r5(主动脉平滑肌细胞)钙化过程中Ghrelin的变化情况,继之观察外源性Ghrelin干预后高脂高糖钙化培养基中A7r5中OPG、RANKL的表达变化。最后观察Ghrelin与OPG/RANKL在钙化发生过程中的调控机制。各组细胞均于培养14天后进行相关检测(Von Kossa染色、茜素红染色、细胞外钙沉积量测定、免疫组织化学染色、Western blot检测)。结果 Von Kossa染色、茜素红染色及细胞外钙沉积量检测显示,高脂高糖及β-甘油磷酸盐模拟的糖尿病代谢紊乱环境下,A7r5钙沉积量显著增加了5.21倍。Ghrelin免疫组织化学染色及IPP6定量分析显示,随着钙化的形成与发展,Ghrelin表达明显下调。外源性Ghrelin可上调OPG相对表达量6.25倍。相对于高脂高糖钙化组,anti-OPG组钙沉积量增加了1.33倍(P0.05),RANKL组钙沉积量增加了1.59倍(P0.05);相对于antiOPG组,anti-OPG+Ghrelin组钙沉积量下调了41.9%(P0.05);相对于RANKL组,RANKL+Ghrelin组钙沉积量下调了57.8%(P0.05)。结论 OPG/RANKL可介导Ghrelin调控A7r5主动脉平滑肌细胞钙化。
[Abstract]:Objective to investigate the regulatory mechanisms of ghrelin (ghrelin) and osteoprotegerin (OPGG) / nuclear factor 魏 B receptor activating factor ligand (RANKL) in the process of calcification.Methods the changes of Ghrelin during calcification of A7r5 (aortic smooth muscle cells) in high fat and high glucose medium were observed at first, and then the expression of A7r5 in high fat and high glucose calcified medium was observed after intervention of exogenous Ghrelin.Finally, the regulatory mechanisms of Ghrelin and OPG/RANKL during calcification were observed.After 14 days of culture, the cells in each group were detected for Von Kossa staining, alizarin red staining, extracellular calcium deposition and immunohistochemical staining.Results Von Kossa staining, alizarin red staining and extracellular calcium deposition were observed.In hyperlipidemic hyperglycemia and 尾 -glycerophosphate simulated diabetic metabolic disorder environment, the calcium deposition of A7r5 increased 5.21-fold. Ghrelin immunohistochemical staining and IPP6 quantitative analysis showed that the expression of ghrelin was down-regulated with the formation and development of calcification.Exogenous Ghrelin could up-regulate the relative expression of OPG by 6.25 times.Conclusion OPG/RANKL can mediate Ghrelin to regulate the calcification of A7r5 aortic smooth muscle cells.
【作者单位】：江苏大学附属医院科教科;江苏大学附属医院心内科;江苏大学附属医院病理科;
【基金】：国家自然科学基金(81370408,81770450) 江苏省自然科学基金(BK20131246) 江苏省卫计委项目(Q201308,QNRC2016836) 江苏省研究生科研创新计划(KYCX17_1801) 镇江市社会发展项目(SH2015038,SH2015023) 江苏大学临床专项(JDLCZX001)
【分类号】：R543.5

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