叶酸对老年冠心病患者外周血内皮祖细胞数量及功能的影响

发布时间：2018-04-02 16:21

本文选题：冠心病　切入点：内皮祖细胞　出处：《中国老年学杂志》2017年17期

【摘要】：目的探讨老年冠心病患者口服叶酸治疗对外周血内皮祖细胞(EPCs)数量及功能的影响。方法选择初次诊断为冠心病的老年患者60例,随机分为治疗组和对照组,每组30例,在规范的冠心病二级预防基础上,治疗组给予叶酸5 mg/d口服,对照组口服安慰剂。在入院时、服药4 w及8 w时检测两组患者的同型半胱氨酸(Hcy)和血流介导的内皮舒张功能(FMD)水平,并利用外周血分离单个核细胞进行体外诱导培养,分别观察其集落形成、增殖及迁移能力。结果 Di L-ac LDL(红色)和FITC-UEA-1(绿色)染色双阳性的细胞可鉴定为EPCs。与对照组比较,治疗组治疗8 w EPCs数量、迁移及增殖能力显著增高[(67.70±4.09)个vs(48.92±3.55)个,(34.03±3.43)%vs(27.54±4.24)%,(0.67±0.04)%vs(0.54±0.04)%,P0.05],FMD水平显著增高[(7.63±1.67)%vs(5.36±1.33)%,P0.05],Hcy水平显著降低[(10.37±0.84)μmol/L vs(12.23±0.74)μmol/L,P0.05]。EPCs数量、迁移、增殖能力与Hcy水平呈负相关(r=-0.668,P0.05;r=-0.657,P0.05;r=-0.775,P0.05),FMD水平与Hcy水平呈负相关(r=-0.718,P0.05)。结论老年冠心病患者口服叶酸可增高外周血EPCs的数量、迁移及增殖能力,改善血流介导的FMD,这可能与叶酸降低Hcy水平有关。
[Abstract]:Objective to investigate the effect of oral folic acid therapy on the number and function of endothelial progenitor cells (EPCs) in elderly patients with coronary heart disease (CHD).Methods Sixty elderly patients with coronary heart disease were randomly divided into treatment group (n = 30) and control group (n = 30). On the basis of standard secondary prevention of coronary heart disease, the treatment group was given folic acid for 5 mg/d and the control group was given placebo.The levels of homocysteine homocysteine (Hcy) and endothelium-mediated endothelium diastolic function (FMDD) were measured at 4 and 8 weeks after admission, and the mononuclear cells isolated from peripheral blood were cultured in vitro, and the colony formation was observed.Proliferation and migration ability.Results the cells with double positive staining of Di L-ac LDL (red) and FITC-UEA-1 (green) could be identified as EPCs.There was a negative correlation between proliferative ability and Hcy level. There was a negative correlation between Hcy level and Hcy level.Conclusion in elderly patients with coronary heart disease, folic acid can increase the number, migration and proliferation of peripheral EPCs, and improve the blood flow mediated FMD.This may be related to the decrease of folic acid level of Hcy.
【作者单位】：东莞市第三人民医院心内科;中山大学附属第一医院心内科;
【基金】：广东省医学科研基金(No.A2011722) 广东省科技厅社会引导发展项目(No.2011B031800011)
【分类号】：R541.4

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