肾素基因MboI多态性与环境因素交互作用对原发性高血压的影响

发布时间：2018-04-08 13:23

本文选题：肾素　切入点：基因　出处：《中华疾病控制杂志》2017年05期

【摘要】：目的探讨肾素基因MboI多态性与环境因素交互作用对原发性高血压的影响。方法采用病例对照研究,高血压病例(1 533例)来源于黑龙江省兰西县农村现场调查,对照(1 557例)来自于同一现场调查的非高血压患者。肾素基因MboI的多态性的检测,主要采用聚合酶链式反应的限制性片段长度多态性(olymerase chain reaction-restriction fragment length polymorphism,PCR-RFLPP)的方法。为确定基因分型的准确性,随机抽取10%样本的PCR产物进行直接测序。结果肾素基因MboI多态性与性别、高血压家族史以及高密度脂蛋白(high density lipoprotein,HDL)之间存在正相乘模型交互作用,ORi(odd rate of interaction)分别为1.36,1.12,1.03;与年龄、腰围(abdomen circumference,AC)、体质指数(body mass index,BMI)以及甘油三酯(triglyceride,TG)之间存在负相乘模型交互作用,ORi分别为0.82,0.47,0.46,0.70。结论男性、有高血压家族史、HDL升高,伴有MboI位点的突变时,均增加高血压的发病风险;年龄、腰围、BMI以及TG水平升高,伴有MboI位点的突变时,均降低高血压的发病风险。
[Abstract]:Objective to investigate the effect of renin gene MboI polymorphism and environmental factors on essential hypertension.Methods A case-control study was conducted in which 1,533 cases of hypertension were investigated in the rural area of Lanxi County, Heilongjiang Province, and 1,557 cases of the control group were collected from non-hypertensive patients investigated in the same field.MboI polymorphism of renin gene was detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLPP).In order to determine the accuracy of genotyping, 10% samples of PCR products were randomly selected for direct sequencing.Results MboI polymorphism of renin gene and sex, family history of hypertension and high density lipoprotein of high density lipoprotein (HDL) had positive multiplicative model interaction. The values of ORiodd rate of interaction were 1.36 and 1.121.03, respectively.There were negative multiplying model interactions between abdomen circumferencea, body mass index (BMI) and triglyceride-triglyceride-triglyceride (TGI).Conclusion the risk of hypertension was increased in men with a family history of hypertension, and increased in age, waist circumference, TG and MboI mutation.
【作者单位】：哈尔滨医科大学公共卫生学院流行病教研室;齐齐哈尔医学院医药科学研究院;中国医学科学院基础学院医学分子生物学国家实验室;
【基金】：黑龙江省自然科学基金项目(D201235)
【分类号】：R544.11

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