巴西苏木素调控自噬对高糖诱导血管内皮细胞损伤的影响

发布时间：2018-04-09 14:13

本文选题：巴西苏木素　切入点：糖尿病大血管病变　出处：《中国动脉硬化杂志》2017年04期

【摘要】：目的探讨巴西苏木素对高糖诱导血管内皮细胞损伤的保护作用及其可能机制,为中药苏木治疗糖尿病大血管病变提供实验依据。方法培养人脐静脉内皮细胞,采用MTT法确定巴西苏木素浓度,高糖诱导损伤后加入巴西苏木素,透射电镜观察自噬体;小管形成实验观察血管生成;蛋白免疫印迹检测自噬相关基因BECLIN1、LC3-Ⅱ、p62蛋白的表达。结果选定用于实验的巴西苏木素浓度为15 mg/L;透射电镜显示高糖组细胞中自噬体数量较对照组少,而巴西苏木素组细胞中自噬体数量较对照组和高糖组多;BECLIN1、LC3-Ⅱ的蛋白表达在高糖组较对照组低,在巴西苏木素组较高糖组高(P0.05);p62的蛋白表达在高糖组较对照组高,在巴西苏木素组较对照组和高糖组低(P0.05)。结论巴西苏木素可能通过调控自噬而对高糖诱导血管内皮细胞损伤发挥保护作用。
[Abstract]:Objective to investigate the protective effect of Sappan Brazil high glucose induced vascular endothelial cell injury and its possible mechanism, to provide experimental basis for Chinese medicine sappan treatment of diabetic macrovascular disease. Methods human umbilical vein endothelial cells, determine the Brazil hematoxylin concentration using MTT method, high glucose induced injury after joining the Brazil hematoxylin, transmission electron microscopy observation of autophagosomes; experiment to observe the angiogenesis formation of tubules; Western blot detection of autophagy related gene BECLIN1, LC3- II, p62 protein expression was selected to experiment in Brazil. Hematoxylin concentration was 15 mg/L; transmission electron microscopy showed that the number of cells in the body of autophagy in high glucose group than in the control group, while the number of Brazil hematoxylin cells autophagy body than the control group and high glucose group; BECLIN1, the expression of LC3- II protein in high glucose group than the control group in Brazil, hematoxylin group compared with high glucose group (P0.05); high p62 protein expression in The high glucose group was higher than the control group, and lower in Brazil hematoxylin group than the control group and the high sugar group (P0.05). Conclusion Brazil hematoxylin may play a protective role in the high glucose induced vascular endothelial cell injury by regulating autophagy.

【作者单位】：锦州医科大学;锦州医科大学附属第一医院中医科;
【基金】：辽宁省自然科学基金资助项目(2015020343)
【分类号】：R54;R587.2

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