运动与甲亢心脏氧应激状态及RyR2表达的差异

发布时间：2018-04-10 01:12

本文选题：甲状腺激素　切入点：适量运动　出处：《郑州大学》2016年硕士论文

【摘要】：背景:甲状腺激素增高诱发的甲亢心脏病与适量运动后心肌肥大对心脏收缩泵血、储备功能的影响常分道扬镳。前者常伴发自由基和抗氧化酶的增高,同时心脏的收缩泵血功能、储备能力增强;而后者常会诱发心脏的收缩和泵血功能减弱等一系列的反应。2012年Burgoyne报道,在心肌氧应激反应中,雷诺定受体2(Ryanodine Receptor 2,Ry R2)及其相关蛋白可起到重要的介导作用,它是影响心脏收缩泵血能力和储备功能的重要因素之一。目的:本研究借助所复制的运动和甲亢诱发的心肌细胞肥大模型,从分子和器官层面探讨氧应激反应和Ry R2表达在生理性心肌细胞肥大、病理性心肌细胞肥大过程中的异同,为临床上甲亢运动疗法提供实验依据。方法:将大鼠随机的分为六组:对照组、轻度甲状腺机能亢进组、重度甲状腺机能亢进组、运动组、轻度甲状腺机能亢进运动组、重度甲状腺机能亢进运动组。对照组用0.9%的生理盐水进行灌胃,轻度甲亢组与重度甲亢组分别行100μg/d与200μg/d左旋甲状腺素灌胃,运动组则绑负6%的自身重量进行游泳锻炼,每天锻炼的时间在30分钟。经过14天后,测定大鼠甲状腺的形态及功能、大鼠心脏的形态及功能、氧化应激反应、Ry R2的表达。结果:1.甲状腺形态和功能:甲亢非运动组大鼠的甲状腺体积变小,质地变韧,甲状腺激素FT3经运动后上述变化均有所回复。2.心脏形态和功能:HE染色显示,运动组大鼠心肌纤维齐整,胶原纤维齐整、变多;甲亢非运动组大鼠心肌细胞粗、长,乱,有断裂,出现炎症细胞;Masson’s显示,胶原纤维多而乱;超声心动图示,甲亢组左心室舒张末期室后壁增厚;ECG示甲亢组R-R间期缩短,HR加快;甲亢运动组大鼠较甲亢非运动组比较,上述变化均有所回复。3.氧应激反应:甲亢组较对照组谷胱甘肽过氧化物酶(Glutathione Peroxidase,GPx)无差异(P0.05),但丙二醛(Malondialdchyde,MDA)含量增高(P0.05);运动组与非运动组比较GPx活力增高和MDA含量均有所增高(P0.05)。4.Ry R2表达:与对照组比较,Ry R2蛋白表达降低(P0.05),甲亢运动组较甲亢非运动组比较,以上的改变发生了一定程度的回复。结论:借助适量的运动能够一定范围内对抗甲状腺激素所造成的氧应激的病理生理作用。
[Abstract]:Background: the effects of hyperthyroidism induced by thyroid hormone elevation and myocardial hypertrophy after exercise on cardiac systolic pump and reserve function are often diverged.The former is often accompanied by increases in free radicals and antioxidant enzymes, as well as an increase in the systolic pump function and reserve capacity of the heart, while the latter often induces a series of responses, such as the contraction of the heart and the weakening of the pump function, Burgoyne reported in 2012.Renolonidine receptor 2(Ryanodine Receptor 2n Ry R2 and its associated proteins may play an important role in the cardiac oxygen stress response, which is one of the important factors affecting the systolic pump capacity and reserve function of the heart.Objective: to investigate the differences and similarities of oxygen stress response and Ry R2 expression in physiologic and pathological cardiomyocyte hypertrophy by using the model of cardiomyocyte hypertrophy induced by exercise and hyperthyroidism.To provide experimental basis for clinical hyperthyroidism exercise therapy.Methods: the rats were randomly divided into six groups: control group, mild hyperthyroidism group, severe hyperthyroidism group, exercise group, mild hyperthyroidism exercise group and severe hyperthyroidism motor group.The control group was perfused with 0.9% normal saline, the mild hyperthyroidism group and the severe hyperthyroidism group received 100 渭 g / d and 200 渭 g / d L-thyroxine intragastric perfusion respectively, while the exercise group took swimming exercise with negative 6% of their own weight, and the exercise time was 30 minutes per day.After 14 days, the morphology and function of rat thyroid, the shape and function of rat heart and the expression of Ry R2 were measured.The result is 1: 1.Thyroid morphology and function: the thyroid volume and texture of hyperthyroidism rats became smaller and toughened, and the changes of thyroid hormone FT3 recovered to a certain extent after exercise.The cardiac morphology and function of rats showed that myocardial fibers in exercise group were even, collagen fibers were even, and collagen fibers were increased in hyperthyroidism group, but in hyperthyroidism non-exercise group, cardiac myocytes were coarse, long, disorderly, broken, inflammatory cells appeared Massonons showed that collagen fibers were more and more chaotic.Echocardiography showed that the left ventricular end diastolic posterior wall thickening in hyperthyroidism group and ECG showed that R-R interval shortened and HR increased in hyperthyroidism group, and that in hyperthyroidism exercise group compared with hyperthyroidism non-exercise group, the above changes recovered to .3.Oxygen stress response: there was no difference between hyperthyroidism group and control group in glutathione peroxidase (Glutathione peroxidase) (GPX), but the content of malondialdehyde (MDA) in hyperthyroidism group was higher than that in control group (P 0.05). The activity of GPx and the expression of MDA in exercise group were higher than those in non-exercise group (P 0.05). 4. Ry R2 expression in hyperthyroidism group was higher than that in control group.The expression of Ry R2 protein in hyperthyroidism group was lower than that in hyperthyroidism group, and the expression of Ry R2 protein in hyperthyroidism group was lower than that in non-exercise group.The above changes have a certain degree of response.Conclusion: moderate exercise can antagonize the pathophysiological effects of oxygen stress induced by thyroid hormone to a certain extent.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R541.85

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