自体与异体脂肪间充质干细胞移植治疗大鼠急性心肌梗死的比较研究

发布时间：2018-04-11 04:33

本文选题：心肌梗死 + 脂肪间充质干细胞　；参考：《解放军医学杂志》2017年09期

【摘要】：目的比较自体与异体脂肪间充质干细胞(ADMSCs)对大鼠急性心肌梗死(AMI)心脏结构以及功能的影响。方法 Lewis雄性大鼠通过结扎左冠状动脉前降支制备急性心肌梗死模型。45只大鼠随机分为模型组(n=15)、自体ADMSCs移植组(n=15)、异体(相同周龄的BN大鼠)ADMSCs移植组(n=15)。AMI模型制作成功1h内将氯甲基苯甲酰胺荧光染料(CM-Dil)标记的第3代脂肪间充质干细胞2×10~6个分三点植入大鼠心肌内,模型组仅注射等量的PBS(0.1ml)。细胞移植后4周进行大鼠心功能检测和免疫荧光、Masson染色等鉴定。结果细胞移植后4周,与模型组比较,两个细胞治疗组左室射血分数明显增加,左室收缩末内径和舒张末内径明显降低(P0.05),胶原沉积分数明显降低(P0.05)。与异体ADMSCs移植组比较,自体ADMSCs移植组左室射血分数增加,心肌梗死区新生毛细血管增加,梗死区心肌组织胶原沉积分数降低(P0.05)。结论自体ADMSCs较异体ADMSCs能够更好地促进梗死区血管增生,通过降低局部胶原沉积、减轻AMI后心肌纤维化程度而抑制心肌梗死后的胶原重构,修复受损心肌组织,改善心脏功能。
[Abstract]:Objective to compare the effects of autogenous adipose mesenchymal stem cells (ADMSCs) on cardiac structure and function in acute myocardial infarction (AMI) rats.Methods Lewis male rats were randomly divided into three groups: model group (n = 15), autologous ADMSCs transplantation group (n = 15) and allogeneic group (n = 15).The third generation adipose mesenchymal stem cells (2 脳 10 ~ 6) labeled with chloromethylbenzoamide fluorescent dye CM-Dil were implanted into rat myocardium in three points within 1 hour.The model group was injected with the same amount of PBS0. 1 ml.Cardiac function and immunofluorescence Masson staining were detected 4 weeks after transplantation.Results at 4 weeks after cell transplantation, the left ventricular ejection fraction (LVEF), left ventricular end-systolic diameter and end-diastolic diameter were significantly decreased, and collagen deposition fraction was significantly decreased in the two groups compared with the model group.Compared with the allogeneic ADMSCs group, the left ventricular ejection fraction (LVEF) increased, the neovascularization increased in the myocardial infarction area, and the collagen deposition fraction in myocardial tissue decreased (P 0.05) in the autologous ADMSCs transplantation group.Conclusion autogenous ADMSCs can promote vascular proliferation in infarcted area better than allogeneic ADMSCs. It can inhibit collagen remodeling after myocardial infarction, repair damaged myocardial tissue and improve cardiac function by reducing local collagen deposition and reducing myocardial fibrosis after AMI.
【作者单位】：军事医学科学院基础医学研究所;海军总医院儿科;海军总医院检验科;
【分类号】：R542.22

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