骨髓间充质干细胞联合螺内酯抑制糖尿病心肌病大鼠细胞凋亡的机制研究

发布时间：2018-04-13 12:53

本文选题：糖尿病心肌病 + 骨髓间充质干细胞　；参考：《临床心血管病杂志》2017年05期

【摘要】：目的:探讨骨髓间充质干细胞联合螺内酯抑制糖尿病心肌病心肌纤维化的可能机制。方法:建立糖尿病心肌病大鼠模型:SD大鼠随机分为对照组与模型组,对照组普通喂养,模型组通过小剂量链脲佐菌素(STZ)及高糖高脂饲养。通过超声、血流动力学、病理染色等评判模型。将构模成功的模型大鼠随机分为3组予以饲养:PBS组(尾静脉注射PBS),干细胞组(干细胞移植),联合组(干细胞移植及螺内酯灌胃)。通过Western Blot检测各组大鼠14-3-3蛋白的表达。结果:(1)通过血糖、血脂、心脏指数、心脏超声及血流动力学检测,判断糖尿病心肌病模型成功;(2)免疫荧光及流式细胞学检测证实干细胞移植到模型大鼠体内;(3)干细胞组及联合组的心肌纤维化要低于PBS组,而14-3-3蛋白表达较PBS组明显上升(均P0.05),联合组更为明显。结论:骨髓干细胞通过上调14-3-3蛋白表达抑制糖尿病心肌病的心肌纤维化,和螺内酯有协同作用。
[Abstract]:Aim: to investigate the possible mechanism of bone marrow mesenchymal stem cells combined with spironolactone in inhibiting myocardial fibrosis in diabetic cardiomyopathy.Methods: the rat model of diabetic cardiomyopathy (DM) rats were randomly divided into two groups: control group and model group. The control group was fed with STZ (low dose streptozotocin) and high glucose and high fat.The models were evaluated by ultrasound, hemodynamics and pathological staining.The successful model rats were randomly divided into 3 groups: 1. PBS group (PBSN via caudal vein), (2) stem cell transplantation group (stem cell transplantation group), (2) combined group (stem cell transplantation and spironolactone intragastric instillation).The expression of 14-3-3 protein was detected by Western Blot.Results Blood glucose, blood lipid, cardiac index, heart ultrasound and hemodynamics were measured.The results of immunofluorescence and flow cytometry showed that the myocardial fibrosis in stem cell group and combination group was lower than that in PBS group.The expression of 14-3-3 protein was significantly higher in PBS group than that in PBS group (P 0. 05, P 0. 05).Conclusion: bone marrow stem cells inhibit myocardial fibrosis in diabetic cardiomyopathy by up-regulation of 14-3-3 protein expression and have synergistic effect with spironolactone.
【作者单位】：武汉市第三医院心内科;武汉市普爱医院;
【分类号】：R542.2;R587.2

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