高血压前期内皮祖细胞数量和迁移增殖能力的性别差异及其与NO关系

发布时间：2018-04-14 08:54

本文选题：内皮祖细胞 + 高血压前期　；参考：《南华大学》2016年硕士论文

【摘要】：目的探讨高血压前期内皮祖细胞数量和迁移增殖能力的性别差异及其与一氧化氮水平的关系。方法招募80例年龄(46.5±4.6)岁的人群作为研究对象,分四组:绝经前健康女性组(21例)、绝经前高血压前期女性组(20例)、健康男性组(19例)和高血压前期男性组(20例),采用流式细胞法定量评估各组KDR和CD34双标阳性的循环内皮祖细胞水平,ac-LDL及lectin荧光标记法定性测定各组体外培养内皮祖细胞数量,检测各组内皮祖细胞的迁移和增殖能力,并测定各组血浆及循环内皮祖细胞分泌的VEGF、NO和GM-CSF水平。结果1.相比健康男性和高血压前期男性,绝经前健康女性和绝经前高血压前期女性内皮祖细胞数量增多,迁移和增殖能力增强,同时其血浆及内皮祖细胞分泌的一氧化氮水平升高(P均0.05)。2.绝经前高血压前期女性循环内皮祖细胞数量和迁移增殖能力与绝经前健康女性相似(P0.05),并两组血浆和内皮祖细胞分泌的一氧化氮水平无明显差异(P0.05)。3.与健康男性相比,高血压前期男性循环内皮祖细胞迁移增殖能力减弱,且血浆和内皮祖细胞分泌的一氧化氮水平降低(P0.05),而健康男性和高血压前期男性内皮祖细胞数量无明显差异(P0.05)。4.四组血浆及内皮祖细胞分泌的血管内皮生长因子和粒-巨噬细胞集落刺激因子水平水平无明显差异(P均0.05)。5.循环内皮祖细胞数量或迁移增殖能力与血浆和内皮祖细胞分泌的NO水平呈明显正相关关系。结论高血压前期内皮祖细胞数量和迁移增殖能力存在性别差异。相比高血压前期男性,绝经前高血压前期女性循环内皮祖细胞数量增多,迁移增殖能力增强,且可能与体内一氧化氮水平呈正相关关系。
[Abstract]:Objective to investigate the relationship between the number of endothelial progenitor cells (EPCs) and migration and proliferation of EPCs and nitric oxide (no) levels in prehypertensive patients.Methods 80 subjects aged 46.5 卤4.6 years were recruited.The patients were divided into four groups: 21 healthy premenopausal women, 20 premenopausal hypertensive women, 19 healthy male) and 20 prehypertensive male patients. Flow cytometry was used to evaluate the positive rates of KDR and CD34 in each group.The level of circulating endothelial progenitor cells (ECs) and lectin fluorescence labeling assay were used to determine the number of ECs cultured in vitro.The migration and proliferation of endothelial progenitor cells in each group were measured, and the levels of VEGF no and GM-CSF in plasma and circulating endothelial progenitor cells were measured.Result 1.Compared with healthy men and prehypertensive men, the number of endothelial progenitor cells in premenopausal healthy women and premenopausal hypertensive women increased, and the ability of migration and proliferation increased.At the same time, the plasma and endothelial progenitor cells secreted nitric oxide levels increased P 0.05. 2.The number and migration and proliferation of circulating endothelial progenitor cells in premenopausal hypertensive women were similar to those in healthy premenopausal women (P 0.05), and the levels of nitric oxide in plasma and endothelial progenitor cells were not significantly different between the two groups.Compared with healthy men, the migration and proliferation of circulating endothelial progenitor cells (ECs) were decreased in prehypertensive men.The levels of nitric oxide secreted by plasma and endothelial progenitor cells decreased (P 0.05), but there was no significant difference in the number of endothelial progenitor cells between healthy men and prehypertensive men.The levels of vascular endothelial growth factor and granulocyte-macrophage colony stimulating factor in plasma and endothelial progenitor cells in the four groups were not significantly different (P > 0.05).There was a positive correlation between the number of circulating endothelial progenitor cells and the level of no secreted by plasma and endothelial progenitor cells.Conclusion there are sex differences in the number of endothelial progenitor cells and the ability of migration and proliferation in preessential hypertension.Compared with prehypertensive male, the number of circulating endothelial progenitor cells in premenopausal women increased and the migration and proliferation ability increased, which may be positively correlated with the level of nitric oxide in the body.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R544.1

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