槲皮素对TNF-α诱导的乳鼠心肌成纤维细胞增殖及P38MAPK、HMGB1蛋白表达的影响

发布时间：2018-04-15 23:17

本文选题：槲皮素 + 成纤维细胞　；参考：《重庆医学》2017年23期

【摘要】：目的研究槲皮素对肿瘤坏死因子α(TNF-α)诱导的心肌成纤维细胞增殖及P38MAPK、高迁移率族蛋白B1(HMGB1)表达的影响。方法通过胰酶消化法和差速贴壁法获得纯化的心肌成纤维细胞,应用四甲基偶氮唑蓝(MTT)法检测心肌成纤维细胞的增殖,采用Western blot法测定P38MAPK、HMGB1蛋白的表达。结果槲皮素对基础状态下的心肌成纤维细胞的增殖无影响,但对TNF-α诱导的心肌成纤维细胞的增殖有抑制作用,且各组细胞的吸光度(A)值随槲皮素浓度的增加而减小(P0.05),各组细胞P38MAPK、HMGB1蛋白表达随槲皮素浓度的增加而减弱(P0.05)。结论槲皮素对TNF-α诱导的心肌成纤维细胞增殖有一定地抑制作用,其机制可能是通过P38MAPK信号通路抑制HMGB1的表达,参与抑制心肌成纤维细胞的增殖。
[Abstract]:Objective to investigate the effects of quercetin on the proliferation of myocardial fibroblasts induced by tumor necrosis factor- 伪 (TNF- 伪) and the expression of p38 MAPK, a high mobility group protein B1 (HMGB1).Methods the purified myocardial fibroblasts were obtained by trypsin digestion and differential adherence method. The proliferation of myocardial fibroblasts was detected by MTT, and the expression of P38 MAPKhmGB1 protein was detected by Western blot.Results Quercetin had no effect on the proliferation of myocardial fibroblasts induced by TNF- 伪, but inhibited the proliferation of myocardial fibroblasts induced by TNF- 伪.With the increase of quercetin concentration, the expression of P38 MAPKhMGB1 protein decreased with the increase of quercetin concentration, and the expression of P38 MAPKhMGB1 protein decreased with the increase of quercetin concentration.Conclusion Quercetin can inhibit the proliferation of myocardial fibroblasts induced by TNF- 伪. The mechanism of quercetin may be to inhibit the expression of HMGB1 through the P38MAPK signaling pathway and participate in the inhibition of the proliferation of myocardial fibroblasts.
【作者单位】：重庆市合川区人民医院心内科;西南医科大学第一附属医院心内科;
【基金】：四川省科技厅国际合作项目(2012HH0011)
【分类号】：R54

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