不同血氧饱和度影响主肺动脉压力变化的离体血管模型研究

发布时间：2018-04-17 10:39

本文选题：高血压 + 肺性　；参考：《中国循环杂志》2017年10期

【摘要】：目的:建立不同血氧饱和度影响主肺动脉压力变化的离体模型,为进一步探索肺动脉高压机制提供研究平台。方法:取新西兰大耳白兔20只,随机选取5只作为正常对照组。其余15只分离肺动脉后将血管置于培养液中,并接入体外循环系统;以新鲜肝素化兔血灌流培养48 h,灌注压力维持40 mm Hg(1 mm Hg=0.133 k Pa);期间通过调节膜肺给氧量控制血氧饱和度,建立3个模型组(n=5):高饱和度组(90%~100%)、中饱和度组(65%~75%)、低饱和度组(40%~50%)。转流结束后获取离体血管和正常对照组兔肺血管行病理形态分析、肺动脉组织IPP6.0定量分析以及匀浆一氧化氮含量分析比较。结果:3个模型组转机时间均达到48 h,压力稳定控制在40 mm Hg,血氧饱和度由高到低分别为(97.94±1.01)%、(72.14±12.85)%、(43.83±8.71)%(P0.05)。病理分析结果显示:与正常对照组兔肺动脉相比,3个模型组离体培养的肺动脉管壁均明显增厚;随着血氧饱和度降低,3个模型组离体培养的肺动脉弹力纤维逐渐增多、增粗,差异均有统计学意义(P0.05)。结论:本实验建立了不同血氧饱和度影响主肺动脉压力变化的离体血管模型。
[Abstract]:Aim: to establish an in vitro model with different blood oxygen saturation influencing the change of pulmonary artery pressure, and to provide a platform for further study on the mechanism of pulmonary hypertension.Methods: 20 New Zealand white rabbits were randomly selected as normal control group.The remaining 15 isolated pulmonary arteries were placed in culture medium and connected to the extracorporeal circulatory system. Fresh heparinized rabbit blood was perfused for 48 hours, and the perfusion pressure was maintained at 40 mm Hg(1 Hg=0.133 k Paan. The oxygen saturation of blood was controlled by adjusting the oxygen supply of the membrane lung.Three model groups were established: high saturation group (90%), middle saturation group (65%) and middle saturation group (65%), and low saturation group (40%).After bypass, the pulmonary blood vessels were obtained and compared with the normal control group. The pathological morphology, IPP6.0 quantitative analysis of pulmonary artery and the content of nitric oxide in homogenate were analyzed and compared.Results: in the three model groups, the conversion time was 48 h, the pressure was controlled at 40 mm Hg, and the blood oxygen saturation from high to low was 72.14 卤12.85 and 43.83 卤8.71, respectively.The results of pathological analysis showed that compared with the normal control group, the pulmonary artery wall of the three model groups was significantly thickened in vitro, and with the decrease of oxygen saturation, the elastic fibers of the pulmonary artery in the three model groups gradually increased and thickened.The difference was statistically significant (P 0.05).Conclusion: in this experiment, an isolated vascular model was established, in which different saturation of blood oxygen affected the change of pulmonary artery pressure.
【作者单位】：北京协和医学院中国医学科学院国家心血管病中心阜外医院心外科;
【基金】：国家自然科学基金(81400041) 高等学校博士学科点专项科研基金(20131106120006)
【分类号】：R544.1

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