超声波辐照诱导蛙腿骨骼肌力学状态变化的初步研究

发布时间：2018-04-21 02:39

本文选题：超声波 + 辐照　；参考：《遵义医学院》2017年硕士论文

【摘要】：目的:观察不同剂量超声波辐照对蛙腿骨骼肌力学状态的影响;量化评价超声波辐照诱导蛙腿骨骼肌力学变化的特征;应用组织多普勒超声成像观察超声波辐照对蛙腿骨骼肌电机械兴奋性及收缩性的影响。方法:(1)制备60个蛙离体坐骨神经-腓肠肌标本,分为A、A’、B、B’四组(15只/组),其中A、B为实验组,A’、B’分别为A、B的空白对照组。实验组接受不同超声波辐照剂量(A组辐照剂量为3.0W/cm2、90s,B组辐照剂量为1.0W/cm2、60s)。将Powerlab多道生理信号采集处理系统与标本连接,发放电刺激坐骨神经诱导腓肠肌收缩,在基础状态张力数据采集后进行超声波辐照,在辐照后第0、第5、第10及第20分钟重复进行张力数据采集。评价以上各组张力测值的时间变化规律以及组间差异。(2)另行制备36个蛙离体坐骨神经-腓肠肌标本,分组(A组和B组各10只/组,A’组和B’组各8只/组)、干预措施及观察时间点同上。所有标本连接Powerlab多道生理信号采集处理系统,同时通过导联线与彩超仪心电通道连接以输入同步电刺激信号。Powerlab发放电脉冲刺激坐骨神经诱导腓肠肌收缩。应用M型彩色组织多普勒及频谱多普勒组织显像同步观察标本腓肠肌在干预前后各个观察时间点的电机械延迟时间(t1)、机械收缩持续时间(t2)及收缩速度(v)。分析评价以上各组三个参数的时间变化规律及组间差异。结果:(1)A组和A’组张力测值差异无统计学意义(P0.05);但交互效应显著(P0.05),对照组A’张力随着时间延长而减低,实验组A张力随着时间延长呈增加趋势。组内比较,A组第5分钟张力较基础状态增高,差异有统计学意义(P0.05)。B组和B’组各观察时间点张力测值差异无统计学意义(P0.05);B组与B’组张力均随时间延长而减低。(2)○1 t1:A组和A’组时间效应、交互效应及分组效应差异均无统计学意义(P0.05);B组和B’组时间效应差异有统计学意义(P0.05),交互效应及分组效应差异无统计学意义(P0.05)。○2 t2:A组和A’组间交互效应及时间效应差异有统计学意义,A’组较A组随时间延长t2呈明显增加趋势(P0.05),分组效应差异无统计学意义(P0.05);B组和B’组时间效应、交互效应及分组效应差异均无统计学意义(P0.05)。○3 v:A组和A’组间时间效应差异有统计学意义,交互效应及分组效应差异无统计学意义;B组和B’组时间效应、交互效应及分组效应均差异无统计学意义。所有组别t1、t2测值均随时间延长呈增加趋势,v测值随时间延长呈降低趋势。结论:当超声波辐照强度为3.0W/cm2、辐照时间90s时,对蛙腿骨骼肌张力有一定的正性肌力作用,辐照后第5分钟肌力明显增高。本研究采用的超声波辐照条件对电机械延迟时间、机械收缩持续时间和收缩速度无显著影响,空白对照组(A’组)较实验组(A组,辐照强度为3.0W/cm2、辐照时间90s)t2增加趋势更明显,间接表明超声波辐照对标本可产生一定正性作用。
[Abstract]:Objective: to observe the effects of different doses of ultrasonic irradiation on the mechanical state of frog leg skeletal muscle, and to evaluate quantitatively the characteristics of the mechanical changes of frog leg skeletal muscle induced by ultrasonic irradiation. The effects of ultrasonic irradiation on the electromechanical excitability and contraction of frog leg skeletal muscle were observed by tissue Doppler ultrasound imaging. Methods 60 isolated Frog sciatic nerve and gastrocnemius muscle specimens were prepared. The experimental group received different doses of ultrasound irradiation. The radiation dose of group A was 3.0 W / cm ~ (2) ~ (90) s ~ (-1). The dose of group B was 1.0 W / cm ~ (2 +) ~ (60) s ~ (-1). The Powerlab multi-channel physiological signal acquisition and processing system was connected with the specimen, and the sciatic nerve was stimulated to induce the contraction of gastrocnemius muscle. Tension data acquisition was repeated at 0, 5, 10 and 20 minutes after irradiation. To evaluate the time variation of tension values and the differences between groups, 36 isolated frog sciatic nerve and gastrocnemius muscle specimens were prepared. Group A and group B were divided into two groups: group A (n = 10) and group B (n = 8). All the specimens were connected to the Powerlab multi-channel physiological signal acquisition and processing system. At the same time, the synchronous electrical stimulation signal .Powerlab was used to stimulate the sciatic nerve to induce gastrocnemius muscle contraction by using the lead wire and the ECG channel of the color ultrasound instrument. M-color tissue Doppler and spectral Doppler tissue imaging were used to observe the electromechanical delay time (t _ 1), mechanical contraction duration (t _ 2) and contraction velocity of gastrocnemius muscle before and after intervention. To analyze and evaluate the time variation of the three parameters and the differences between the three groups. Results there was no significant difference in tension between group A and group A (P 0.05), but the interaction effect was significant (P 0.05). The tension of group A decreased with time, and tension of group A increased with time. The tension in group A at 5 minutes was higher than that in basic state. There was no significant difference in the observed point tension values between group B and group B (P 0.05). The tension of group B and group B 'decreased with time, and the time effect of group A and group A' decreased with the prolongation of time. There was no significant difference in the interaction effect and the group effect. There was no significant difference in the time effect between the B group and the B 'group. There was no significant difference in the interaction effect and the group effect. There was no significant difference in the interaction effect between group A and group A and the interaction effect between group A and group A' was timely. There was significant difference in the effect between group A and group A (P 0.05), but there was no significant difference between group A and group B (P 0.05), and there was no significant difference in group B and group B'in time effect between group A 'and group A (P < 0.05), but there was no significant difference between group A and group A (P < 0.05). There was no significant difference in the time effect between group A and group A, but there was no significant difference in time effect between group B and group A, but there was no significant difference in time effect between group A and group A, but there was no significant difference in time effect between group B and group B', but there was no significant difference in time effect between group A and group A. There was no significant difference in interaction effect and group effect. The values of T1 and T2 in all groups showed an increasing trend with time prolonging and the values of v showed a decreasing trend with time prolonging. Conclusion: when the intensity of ultrasound irradiation is 3.0 W / cm ~ 2 and irradiation time is 90 s, it has a positive effect on the skeletal muscle tension of frog leg, and the muscle strength of frog leg increases obviously at 5 minutes after irradiation. The ultrasonic irradiation conditions adopted in this study had no significant effect on the electromechanical delay time, mechanical contraction duration and contraction velocity. The intensity of irradiation was 3.0W / cm ~ 2, and the increasing trend of irradiation time 90s)t2 was more obvious than that of group A (blank control group). The results showed that ultrasonic irradiation had positive effect on the specimen.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.6;R445.1

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