可溶性环氧化物水解酶抑制剂在TGF-β1诱导的脐静脉内皮细胞发生内皮—间质转化中的作用

发布时间：2018-04-23 19:04

本文选题：可溶性环氧化物水解酶抑制剂 + 内皮-间质转化　；参考：《郑州大学》2017年硕士论文

【摘要】：背景心肌纤维化(cardinal fibrosis,MF)是指在多种病理因素作用下心肌成纤维细胞的过度增殖、胶原纤维的过度沉积、比例失调及异常分布所致的心脏间质重构。MF是心脏对各种刺激产生的适应性反应,也是心肌缺血、心肌病、心力衰竭、糖尿病、高血压、肺动脉高压等多种心血管疾病发展至一定阶段共同的病理生理改变。多种机制参与心肌纤维化的进程,包括肾素-血管紧张素-醛固酮系统(renin-angiotensin-aldosterone system,RAAS)、内皮素(endothelin,ET)、核因子κB(nuclear factorκB,NF-κB)、一氧化氮(nitric oxide,NO)、转化生长因子-β(transforming growth factor-β1,TGF-β)等。减少心肌重构能够改善患者的预后,因此寻找特异性的抗心肌纤维化药物,减少心肌重构成为了当前研究的热点。近些年来,随着对心肌纤维化发生机制认识的逐步深入,内皮-间质转化(endothelial mesenchymal transition,EndMT)在心肌纤维化中的作用受到越来越多研究者的重视。内皮-间质转化属于上皮-间质转化(Epithelial mesenchymal transition,EMT)的一种特殊类型,是内皮细胞在多种因素刺激下逐渐失去其形态和功能,向间充质细胞表型转化并获得增殖、迁移及合成胶原等功能的过程。TGF-β信号、Notch信号、Wnt信号、MicroRNAs及一些炎症因子等可能参与EndMT的调节,其中TGF-β1/Smads信号通路是研究最为深入的一种。环氧化物水解酶(epoxide hydratase,EH)普遍存在于哺乳动物体内,作用于心血管疾病的多个环节,具有舒张血管、抗炎、刺激血管形成等作用。可溶性环氧化物水解酶抑制剂(soluble epoxied hydrolase inhibitor,sEHI)是EH的抑制剂,参与抑制多环芳烃环氧化物、环氧二十碳三烯酸(epoxyeicosatrienoic acid,EET)等环氧化物的代谢,它具有抗心肌纤维化、调节血脂、抗高血压及减少心肌缺血再灌注损伤等作用。然而sEHI是否可以通过抑制EndMT发挥抗心肌纤维化作用及其抗纤维化作用是否是通过抑制TGF-β1/Smads信号通路来实现尚无人报道。目的本研究采用TGF-β1诱导人脐静脉内皮细胞(human umbilical vein epithelial cell,HUVEC-12)发生内皮-间质转化,并给予不同剂量的t-AUCB进行干预。通过CCK-8法观察细胞活性,real time RT-PCR检测内皮、间质标志物和TGF-β1/Smads下游转录因子的基因表达,光镜观察HUVEC-12细胞形态,Western blot检测Smad2/3的磷酸化水平。初步探讨t-AUCB抗纤维化作用与TGF-β1/Smads信号通路的相关性,为t-AUCB抗心肌纤维化的应用提供细胞实验依据。材料与方法人脐静脉内皮细胞株HUVEC-12购自YRGene(NC006),在37℃、5%CO2的培养箱中培养。实验前将细胞置于无血清培养基中进行6h的饥饿处理。然后将其分为四组:对照组(无血清DMEM培养液持续培养)、TGF-β1组(含10μg/L TGF-β1的无血清DMEM培养液培养72h)、t-AUCB干预组(先采用含50μmol/L t-AUCB无血清DMEM培养液预保护40min,后采用含10μg/L TGF-β1及50μmol/L t-AUCB的无血清DMEM培养液共同培养72h)、t-AUCB(含50μmol/L t-AUCB无血清DMEM培养液持续培养72h)。各组细胞培养24h时,采用Western blot检测各组细胞Smad2/3的磷酸化水平。各组细胞培养72h时,应用细胞毒性试剂盒(cell counting kit-8,CCK-8)检测细胞活性;光镜观察细胞形态的变化;实时荧光定量PCR(Real-time PCR,RT-PCR)检测内皮标志物CD31及间质标志物collagen I、collagenIII、vimentin的基因表达和TGF-β1/Smads信号通路中下游转录因子snail 1、twist 1、twist 2、ZEB1的基因表达。结果(1)TGF-β1(10μg/L)和/或t-AUCB(1、10、50μmol/L)对细胞活性影响差异无统计学意义(P均0.05)。(2)real time RT-PCR结果提示,TGF-β1(10μg/L)诱导培养HUVEC-12细胞72 h后,内皮细胞标记物CD31的基因表达显著下调,间质标记物collagen I、collagen III、vimentin的基因表达则显著上调;TGF-β1(10μg/L)与t-AUCB(1、10、50μmol/L)共同干预细胞72 h后可显著逆转这种现象,且具有浓度依赖性(P均0.05)。(3)光镜结果示,对照组细胞光镜下为鹅卵石形,细胞间连接紧密;TGF-β1干预组细胞转变为狭长形,细胞间隙疏松;TGF-β1与t-AUCB(50μmol/L)联合干预细胞及单用t-AUCB干预细胞72h后,细胞形态与对照组相似。(4)Western blot结果提示,TGF-β1(10μg/L)干预HUVEC-12细胞72h后可显著提高Smad2/3的磷酸化水平,t-AUCB(50μmol/L)则可显著逆转上述变化(P均0.05)。(5)real time RT-PCR结果提示,与对照组相比,TGF-β1(10μg/L)诱导组细胞下游转录因子snail1、twist1、twist2、ZEB1的基因表达显著增高;t-AUCB(50μmol/L)与TGF-β1(10μg/L)联合干预时则可显著抑制上述基因的表达(P均0.05)。结论(1)TGF-β1可诱导的HUVEC-12细胞发生间充质细胞表型的转化。(2)t-AUCB对HUVEC-12细胞发生EndMT无明显影响。(3)t-AUCB可抑制TGF-β1诱导的HUVEC-12细胞发生EndMT转化。
[Abstract]:Background myocardial fibrosis (cardinal fibrosis, MF) refers to the excessive proliferation of myocardial fibroblasts under various pathological factors, excessive deposition of collagen fibers, disproportions and abnormal distribution of cardiac interstitial remodeling.MF is the adaptive response of the heart to various stimuli. It is also the myocardial ischemia, cardiomyopathy, heart failure, and diabetes. A variety of cardiovascular diseases, such as disease, hypertension, and pulmonary artery hypertension, develop to a certain stage of common pathophysiology. Many mechanisms are involved in the process of myocardial fibrosis, including the renin angiotensin aldosterone system (renin-angiotensin-aldosterone system, RAAS), endothelin (ET), nuclear factor kappa B (nuclear factor kappa B, NF- kappa B), Nitric oxide (nitric oxide, NO), transforming growth factor - beta (transforming growth factor- beta 1, TGF- beta) and so on. Reducing myocardial remodeling can improve the prognosis of patients. Therefore, finding specific anti myocardial fibrosis drugs and reducing myocardial weight constitute the hot spot of current research. Step by step, the role of endothelial mesenchymal transition (EndMT) in myocardial fibrosis is being paid more attention by more and more researchers. Endothelium transformation belongs to a special type of Epithelial mesenchymal transition (EMT), which is the gradual loss of endothelial cells under a variety of factors. Morphology and function,.TGF- beta signal, Notch signal, Wnt signal, MicroRNAs and some inflammatory factors may be involved in the regulation of EndMT, and TGF- beta 1/Smads signaling pathway is the most in-depth study. Epoxide hydrolase (epoxide hydratase, epoxide hydratase,) EH (soluble epoxied hydrolase inhibitor, sEHI) is an inhibitor of EH, which is an inhibitor of EH, and is involved in the inhibition of polycyclic aromatic epoxides, epoxy twenty carbon three enoic acid (epoxyei). Cosatrienoic acid, EET) and other epoxides metabolism, it has the effect of anti myocardial fibrosis, regulating blood lipid, anti hypertension and reducing myocardial ischemia reperfusion injury. However, whether sEHI can inhibit the effect of anti fibrosis by inhibiting EndMT and its anti fibrosis effect by inhibiting the TGF- beta 1/Smads signaling pathway to achieve this. The purpose of this study was to use TGF- beta 1 to induce endothelial cell transformation in human umbilical vein endothelial cells (human umbilical vein epithelial cell, HUVEC-12), and to give different doses of t-AUCB to intervene. The activity of cells was observed by CCK-8 method. Real time RT-PCR was used to detect the inner skin, interstitial markers and downstream transcription factors. The morphology of HUVEC-12 cells was observed by light microscopy, and the phosphorylation level of Smad2/3 was detected by Western blot. The correlation between the anti fibrosis effect of t-AUCB and the TGF- beta 1/Smads signaling pathway was preliminarily discussed, and the experimental basis for the application of t-AUCB against myocardial fibrosis was provided. Materials and methods of human umbilical vein endothelial cells were purchased from YRGene (NC006). The cells were cultured in the 5%CO2 culture box at 37 degrees C. Before the experiment, the cells were placed in the serum-free medium for 6h starvation. Then they were divided into four groups: the control group (serum-free DMEM culture medium continuous culture), the TGF- beta 1 group (including the serum free DMEM culture solution of 10 mu g/L TGF- beta 1), and the t-AUCB intervention group (50 micron mol/L t-AUCB without serum-free DME). M culture solution preprotected 40min, then cultured 72h containing 10 mu g/L TGF- beta 1 and 50 mu mol/L t-AUCB, and t-AUCB (containing 50 mu t-AUCB serum DMEM culture liquid continuously). The cell activity was detected by cell counting kit-8 (CCK-8); the changes in cell morphology were observed by light microscopy; real-time quantitative PCR (Real-time PCR, RT-PCR) was used to detect the endothelial marker CD31 and the interstitial marker collagen I, collagenIII, the downstream transcription factor 1, and 1 T 2, ZEB1 gene expression. Results (1) there was no significant difference in the effect of TGF- beta 1 (10 mu g/L) and / or t-AUCB (1,10,50 mu mol/L) on cell activity (P 0.05). (2) real time RT-PCR results suggested that TGF- beta 1 (10 micron) induced the cultured cell 72 Agen III, vimentin gene expression was significantly up-regulated, and TGF- beta 1 (10 mu g/L) and t-AUCB (1,10,50 mu mol/L) could significantly reverse this phenomenon after the co intervention of 72 h (P 0.05). (3) the light mirror results showed that the cells in the control group were goose egg shaped and the cells were closely connected; TGF- beta 1 intervention group changed into a narrow shape, Cell space was loose; after TGF- beta 1 and t-AUCB (50 mol/L) intervened cells and single t-AUCB intervention cells 72h, the cell morphology was similar to that of the control group. (4) Western blot results suggested that TGF- beta 1 (10 u g/L) could significantly improve the phosphorylation of Smad2/3. (50 micron) could significantly reverse the above changes (0.05). 5) real time RT-PCR results showed that the gene expression of downstream transcription factor Snail1, Twist1, Twist2 and ZEB1 increased significantly in TGF- beta 1 (10 mu g/L), while t-AUCB (50 mu mol/L) and TGF- beta 1 (10 mu) could significantly inhibit the expression of the above gene (0.05). Conclusion (1) beta 1 induced cell hair. Transformation of the phenotype of mesenchyme cells. (2) t-AUCB has no significant effect on the occurrence of EndMT in HUVEC-12 cells. (3) t-AUCB can inhibit the EndMT transformation of HUVEC-12 cells induced by TGF- beta 1.

【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R542.2

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