美托洛尔对左西孟旦在大鼠心脏正性肌力效应的干预研究

发布时间：2018-04-26 14:04

本文选题：β受体阻断剂 + 美托洛尔　；参考：《山西医科大学》2017年硕士论文

【摘要】：目的:在大鼠离体心脏及在体条件下观察美托洛尔对左西孟旦正性肌力作用的影响。方法:本实验以正常SD大鼠为实验对象,用离体灌流的方法研究不同浓度左西孟旦(30nmol/L、300nmol/L、3000nmol/L)和美托洛尔(4μmol/L、20μmol/L、100μmol/L)单独作用或二者联合作用对大鼠离体心脏心率(HR)、左心室发展压(LVDP)、左室内压上升最大速率(+dp/dtmax)、左室内压下降最大速率(-dp/dtmax)等心功能指标的影响;于在体条件下观察不同浓度的左西孟旦(5,10,20ug/kg/min)静脉推注和口服不同剂量美托洛尔(5,10,20mg/kg)单独作用或二者联合作用对健康大鼠心功能(HR、+dp/dtmax、-dp/dtmax、LVDP)效应的影响。结果:1.在离体心脏灌流及在体动物实验中,中等剂量的左西孟旦明显增加+dp/dtmax、-dp/dtmax、LVDP等指标,但对HR没有变化,大剂量左西孟旦增加大鼠心功能的同时引起心率加快(P0.05)。2.在离体心脏灌流中研究中,美托洛儿对大鼠心脏功能各项指标没有明显影响,未发现左西孟旦组与加用美托洛尔组在心率、LVDP等指标之间存在显著差异(P0.1)。3.在体动物实验中,美托洛儿明显减弱大鼠心功能各项指标,加用美托洛尔后可减慢大剂量左西孟旦引起的心动过速(P0.05),但对左西孟旦引起的+dp/dtmax、-dp/dtmax、LVDP增加没有影响(P0.1)。结论:在离体心脏灌流及在体动物实验中,左西孟旦明显改善大鼠心功能,但大剂量的左西孟旦可引起心动过速,在体条件下美托洛尔可对抗左西孟旦引起的心动过速作用,但左西孟旦引起的正性心肌收缩力效应不会减弱。
[Abstract]:Aim: to observe the effect of metoprolol on the positive inotropic effect of levosimendan in isolated rat heart and in vivo. Methods: normal SD rats were used as experimental objects. In vitro perfusion method was used to study the effects of 30 nmol / L 30 nmol / L of 30 nmol / L of different concentrations and 4 渭 mol / L of metoprolol 4 渭 mol / L ~ 20 渭 mol / L ~ (100 渭 mol / L) on heart rate (HRT), left ventricular development pressure (LVDP), left ventricular pressure (LVDP), maximum rate of left ventricular pressure rise (DP / DT _ (max) and left ventricular pressure (LV / L) in vitro in rats. The influence of cardiac function indexes such as decreasing maximum velocity and dp / dtmax. etc. The effects of intravenous injection and oral administration of metoprolol on HRD, dp- dtmax-dpdtmax-dpdtmaxLVDPP of healthy rats were observed in vivo. The result is 1: 1. In isolated perfused hearts and in vivo animal experiments, middle dose of levosimendan significantly increased DP / dtmax-dp-dtmax-LVDP, but did not change HR. Large dose of levosimendan increased cardiac function of rats and caused heart rate to accelerate (P0.05?. 2). In the study of isolated cardiac perfusion, metoprolol had no significant effect on the cardiac function of rats. There was no significant difference in heart rate and LVDP between levosimendan group and metoprolol group. In vivo animal experiments, metoprolol significantly weakened the cardiac function of rats, and metoprolol could slow down the tachycardia induced by high dose of levosimendan (P0.05), but had no effect on the increase of DP / dtmax-dtmax / dtmax LVDP induced by levosimendane-dtmax / dt0. 1. Conclusion: in isolated cardiac perfusion and in vivo animal experiments, levosimendan can obviously improve cardiac function of rats, but large dose of levosimendan can induce tachycardia, and metoprolol can antagonize the tachycardia induced by levosimendan in vivo. But the positive contractility effect caused by levosimendan is not weakened.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.6

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