螺内酯对心肌肥厚大鼠左心室可溶性ST2及核转录因子_κB的影响

发布时间：2018-04-30 04:19

本文选题：螺内酯 + 心肌肥厚　；参考：《中华高血压杂志》2017年09期

【摘要】：目的探讨螺内酯对心肌肥厚大鼠可溶性ST2(sST2)及核转录因子_κB(NF-_κB)的影响。方法选取健康雄性SD大鼠30只,随机分为对照组(n=10)、模型组(n=10)、药物干预组(n=10)。对照组大鼠皮下注射生理盐水;模型组皮下注射异丙肾上腺素(ISO);药物干预组皮下注射ISO,同时给予螺内酯20mg/(kg·d)灌胃。14d后处死大鼠,计算心脏质量/体质量、左心室质量/体质量,观察心肌细胞直径;免疫组化及逆转录聚合酶链反应(RT-PCR)法检测心肌组织中sST2及NF-_κB的表达;Masson染色观察心肌纤维化程度。结果与对照组比,模型组心脏质量/体质量、左心室质量/体质量、心肌细胞直径升高[(3.39±0.11)比(2.47±0.07)mg/g,(2.34±0.11)比(1.78±0.07)mg/g,(26.30±0.95)比(14.53±0.47)μm;均P0.05];sST2与NF-_κB mRNA和蛋白表达升高(均P0.05)。与模型组比较,药物干预组心脏质量/体质量、左心室质量/体质量、心肌细胞直径降低[(2.79±0.07)比(3.39±0.11)mg/g,(1.92±0.10)比(2.34±0.11)mg/g,(20.96±0.65)比(26.30±0.95)μm;均P0.05],sST2与NF-_κB mRNA和蛋白表达降低(均P0.05)。心肌纤维化程度:模型组大于对照组,干预组小于模型组。结论螺内酯可能通过调节sST2的表达,抑制下游NF-_κB的表达,对抗ISO诱导的大鼠心肌肥厚。
[Abstract]:Objective to investigate the effects of spironolactone on soluble ST2s ST2 and NF-魏 B in rats with myocardial hypertrophy. Methods 30 male Sprague-Dawley rats were randomly divided into control group (n = 10), model group (n = 10) and drug intervention group (n = 10). Rats in the control group were subcutaneously injected with normal saline; the model group were injected with isoproterenol isoprenaline; the drug intervention group was given subcutaneous injection of ISO, and spironolactone (20mg/(kg d) was given intragastrically for 14 days. The rats were killed to calculate the heart mass / body weight, left ventricular mass / body mass. The diameter of myocardial cells and the expression of sST2 and NF-kappa B in myocardium were detected by immunohistochemistry and reverse transcription-polymerase chain reaction RT-PCR.Masson staining was used to observe the degree of myocardial fibrosis. Results compared with the control group, the cardiac weight / body weight, left ventricular mass / body mass, cardiac myocyte diameter in the model group increased [3.39 卤0.11] vs 2.47 卤0.07 mg / g / 0.11, respectively, compared with 1.78 卤0.07 mg / g / kg / kg group, respectively, and the expression of SST _ 2 and NF-kappa B mRNA and protein were significantly increased (P < 0.05). Compared with the model group, the cardiac weight / body weight, left ventricular mass / body weight, cardiac myocyte diameter in the drug intervention group decreased [2.79 卤0.07] vs 3.39 卤0.11 mg / g / g (1.92 卤0.10), compared with 2.34 卤0.11 mg / g / 0.65 vs 26.30 卤0.95) 渭 m; P0.05] the expression of sST2 and NF-kappa B mRNA and protein decreased (P 0.05). The degree of myocardial fibrosis: the model group was larger than the control group, and the intervention group was smaller than the model group. Conclusion Spironolactone may inhibit the expression of downstream NF-kappa B by regulating the expression of sST2 and antagonize myocardial hypertrophy induced by ISO in rats.
【作者单位】：郑州市中心医院心血管内科;郑州大学第二附属医院心内科;
【分类号】：R544.1

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