合并流出道狭窄的腹主动脉瘤动物模型建立

发布时间：2018-05-01 14:07

本文选题：腹主动脉瘤 + 流出道狭窄　；参考：《山西医科大学》2017年硕士论文

【摘要】：目的:实验旨在建立合并流出道狭窄(OTS)的兔腹主动脉瘤(AAA)动物模型,探讨本模型特点、AAA破裂的高危因素及OTS对AAA病理影响,为腹主动脉瘤的治疗和研究提供实验基础。方法:24只雄性新西兰大白兔随机平均分成A、B、C,3组,每组8只。A组为AAA组,游离部分肾下腹主动脉,猪胰蛋白酶从外膜浸润该区域30分钟;B组为合并流出道狭窄的腹主动脉瘤(OTS-AAA)组,在A组的基础上于酶浸润区域远端行腹主动脉缩窄。C组为假手术组,采用生理盐水替代胰蛋白酶进行同时长浸润,并于完成结扎缩窄后剪断结扎线。于术后2周行动脉造影(DSA),取材,对标本进行组织病理分析。结果:术后14天,A组,7例形成AAA,平均动脉直径3.53±0.03mm。B组,6例形成AAA。术后18h、29h、68h,分别有1只动物发生腹主动脉瘤破裂死亡,为破裂组(Br组),其平均动脉直径3.57±0.07mm。其余3例,为非破裂组(Bu组),平均动脉直径3.49±0.08mm。C组,未见明显腹主动脉扩张,平均腹主动脉直径2.32±0.12mm。A、B组间及Br、Bu组间动脉瘤直径无统计学差异(p=0.312,0.056)。相较C组,B组和A组均出现明显腹主动脉瘤样扩张及动脉壁破环、炎性细胞浸润及弹力纤维断裂。结论:两种方法均能可靠的建立AAA模型,OTS-AAA模型较经典模型更易引起瘤体破裂,在一定程度上可应用于AAA破裂危险因素的研究。
[Abstract]:Objective: to establish a rabbit model of abdominal aortic aneurysm (AAA) with outflow tract stenosis (OTS), and to investigate the high risk factors of AAA rupture and the pathological effect of OTS on AAA, and to provide experimental basis for the treatment and study of abdominal aortic aneurysm. Methods Twenty four male New Zealand white rabbits were randomly divided into three groups: group A (n = 8), AAA group (n = 8), and group A (n = 8). Porcine trypsin infiltrated the area from the adventitia for 30 minutes. Group B was divided into two groups: OTS-AAA group with outflow tract stenosis. On the basis of group A, abdominal aorta coarctation was performed at the distal end of the enzymatic infiltration area. Group C was sham-operated group. Saline was used instead of trypsin for simultaneous long infiltration, and the ligation line was cut off after ligation and constriction. Two weeks after operation, arterial angiography was performed and the specimens were taken for histopathological analysis. Results: on the 14th day after operation, 7 patients in group A and 6 patients in group A with mean arterial diameter of 3.53 卤0.03mm.B. One animal died of abdominal aortic aneurysm rupture at 18h, 29h and 68h, the mean diameter of the artery was 3.57 卤0.07mm. In the other 3 cases, there was no significant dilatation of abdominal aorta in the mean arterial diameter of 3.49 卤0.08mm.C group, and there was no significant difference in the diameter of aneurysm between group B and Br-Bu group (2.32 卤0.12mm.An) and between the group of Br-Bu and the group of Br-Bu, there was no significant difference in the diameter of aneurysms between the two groups. Compared with group C, group B and group A showed obvious abdominal aortic aneurysm like dilatation, rupture of arterial wall, infiltration of inflammatory cells and rupture of elastic fibers. Conclusion: the two methods can be used to establish AAA model, OTS-AAA model is more likely to cause tumor rupture than classical model, and can be used to study the risk factors of AAA rupture to some extent.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R543.16;R-332

【相似文献】