褪黑素对实验性动脉粥样硬化兔心脏的保护作用及其可能的分子机制

发布时间：2018-05-04 21:40

本文选题：动脉粥样硬化 + 心肌组织　；参考：《安徽医科大学学报》2017年12期

【摘要】：目的观察褪黑素(MLT)对实验性动脉粥样硬化兔心脏的保护作用及其可能的分子机制。方法普通级纯种新西兰大耳白兔随机分为3组,正常组:饲喂普通饲料;模型组:饲喂高脂饲料(普通饲料+1%胆固醇+5%猪油);褪黑素组:饲喂高脂饲料的同时MLT灌胃10 mg/(kg·d)治疗。纯种大耳白兔适应性饲养一周后,采用高脂饮食诱导造模,同时给予褪黑素药物治疗。于12周后处死进行采血,检测血清中血脂等指标。取动脉进行油红染色,检测脂质斑块形成情况;取心脏组织,包埋后HE染色观察心肌组织的形态变化;Masson染色检测心肌组织中胶原纤维的变化;Western blot法检测p38/丝裂原活化蛋白激酶(MAPK)通路相关蛋白及肌球蛋白轻链激酶(MLCK)、肌球蛋白轻链(MLC)、肌球蛋白轻链磷酸化(p-MLC)的表达水平。结果动脉油红染色结果显示模型组出现大量脂质斑块,证明造模成功。血脂结果显示模型组总胆固醇(TCH)、三酰甘油(TG)浓度明显上升,而褪黑素组相对模型组下降。油红染色HE染色结果显示MLT能改善心肌组织形态的紊乱情况,Masson染色结果显示MLT能缓解心肌组织胶原纤维的堆积。Western blot结果显示MLT能下调p38的磷酸化水平以及MLCK蛋白的表达水平,以及下调MLC的磷酸化表达水平(P0.05)。结论 MLT可能通过降低MAPK通路中相关蛋白p38的磷酸化调控MLCK蛋白的表达,从而降低MLC的磷酸化水平对动脉粥样硬化兔心肌组织起到保护作用。
[Abstract]:Objective to investigate the protective effect of melatonin (MLT) on experimental atherosclerosis rabbit heart and its possible molecular mechanism. Methods New Zealand white rabbits were randomly divided into 3 groups. The model group was fed with high fat diet (1% cholesterol 5% lard) and melatonin group (fed with high fat diet and MLT for 10 mg/(kg). Purebred white rabbits were fed adaptively for one week, then induced by high fat diet and treated with melatonin. After 12 weeks, blood was collected and blood lipid was detected. The arteries were stained with oil red to detect the formation of lipid plaques. Morphological changes of Myocardial tissue observed by HE staining after embedding; changes of Collagen Fibers in Myocardial tissue by Masson staining; Detection of p38 / Mitogen-activated protein Kinase MAPK Pathway related proteins and Myosin Light chain Kinase (MLCKK), Myosin by Western blot The expression level of MLC, myosin light chain phosphorylated P-MLC. Results A large number of lipid plaques were found in the model group, which proved that the model was successful. The results showed that the concentrations of TCHG and TG in the model group were significantly increased, while those in the melatonin group were lower than those in the model group. The results of oil red HE staining showed that MLT could improve the disorder of myocardial morphology. The results showed that MLT could alleviate the accumulation of collagen fibers in myocardium. Western blot showed that MLT could down-regulate the phosphorylation of p38 and the expression of MLCK protein. The phosphorylation level of MLC was down-regulated (P 0.05). Conclusion MLT may play a protective role in myocardial tissue of atherosclerotic rabbits by decreasing the phosphorylation of p38 in MAPK pathway and regulating the expression of MLCK protein.
【作者单位】：安徽医科大学分子生物学实验室安徽省/省部共建教育部重要遗传病基因资源利用重点实验室;
【基金】：国家自然科学基金(编号:81570419) 安徽省高校省级自然科学研究项目(编号:KJ2013Z127)
【分类号】：R54

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