失血性心脏骤停兔模型的构建

发布时间：2018-05-06 16:42

本文选题：失血 + 心脏骤停　；参考：《广东医学》2017年08期

【摘要】：目的构建失血性心脏骤停兔模型,以满足失血性心脏骤停基础研究的需要。方法选择27只新西兰兔分为假手术组(A组,无放血、无复苏)、未控制组(B组,放血但无复苏)、液体复苏组(C组,放血及复苏)。A组不作处理,B、C组经动静脉放血约放出50%的全身血量,达到心脏骤停后维持状态15 min,随后采用乳酸林格液和全血回输复苏。各时相点采动脉血测血气分析并统计输液量、失血量及6 h存活率。结果建模成功时B、C组血压显著降低,C组平均动脉压(MAP)下降至(14.5±5.3)mmHg。30 min MAP为(69.75±14.37)mmHg。在复苏5 min时pH值、动脉二氧化碳分压(PaCO_2)、红细胞压积(Hct)、血红蛋白(Hb)、实际碳酸氢盐(AB)、标准碳酸氢盐(SB)、实际碱剩余(ABE)、标准碱剩余(SBE)显著降低,K~+、乳酸水平显著升高(与0 min比较:P0.01);在复苏25 min,仅K~+恢复至0 min水平,而AB、SB、SBE、ABE与乳酸均低于0 min水平(P0.05)。Ca~(2+)在所测时间点皆与T0时间点比较差异无统计学意义(P0.05)。结论通过动静脉联合放血构建的模型稳定性和可重复性高,适用于失血性心脏骤停的研究。
[Abstract]:Objective to establish a rabbit model of hemorrhagic cardiac arrest to meet the needs of basic research on hemorrhagic cardiac arrest. Methods Twenty-seven New Zealand rabbits were divided into two groups: sham operation group (group A), no bleeding, no resuscitation, no control group (group B), bleeding without resuscitation and fluid resuscitation group (group C). About 50% of the whole body blood volume was released through arteriovenous bleeding in group A and resuscitation group A, which was maintained for 15 min after cardiac arrest, and then was resuscitated with Ringer lactate solution and whole blood transfusion. Arterial blood was collected at each time point for blood gas analysis and blood transfusion volume, blood loss and 6 h survival rate were counted. Results when the model was successfully established, the mean arterial pressure in BPC group was significantly decreased to 14.5 卤5.3)mmHg.30 min MAP (69.75 卤14.37mmHg). At 5 min after resuscitation, pH value, arterial partial pressure of carbon dioxide (Paco _ 2), hematocrit (HCT), hemoglobin (HB), actual bicarbonate (ABN), standard bicarbonate (SBE), actual base residue (ABE), standard base residual (SBE) decreased significantly. The level of lactic acid increased significantly (compared with 0 min, only K0 min was recovered to 0 min level at 25 min after resuscitation, but both ABN SBE and lactic acid were lower than 0 min level P0.05 路CaP0 2). There was no significant difference between the measured time points and T0 time points (P 0.05). Conclusion the model constructed by combined arteriovenous bleeding has high stability and reproducibility and is suitable for the study of hemorrhagic cardiac arrest.
【作者单位】：南方医科大学南方医院急诊科;
【基金】：广东省科技计划项目(编号:2013B010401014,2014A020212192)
【分类号】：R-332;R541.78

【相似文献】