CD36在氧化低密度脂蛋白降低鼠源细胞锰超氧化物歧化酶活性中的作用

发布时间：2018-05-07 19:48

本文选题：CD + 氧化低密度脂蛋白(ox-LDL)　；参考：《中国老年学杂志》2017年13期

【摘要】：目的探讨CD36在氧化低密度脂蛋白(ox-LDL)降低大鼠锰(Mn)超氧化物歧化酶(SOD)活性中的作用。方法将鼠源RAW264.7巨噬细胞按照处理方式分为ox-LDL处理组和抗CD36拮抗组;ox-LDL处理组在培养液中加入100 mg/L ox-LDL,抗CD36拮抗组在培养液中先加入2 mg/L抗CD36 m Ab预处理1 h,再加入100 mg/L ox-LDL。实验结束后比较两组细胞凋亡率和Mn-SOD活性。结果 ox-LDL处理组24、48 h的细胞凋亡率[分别为(10.8±2.91)%与(29.88±4.47)%]明显高于抗CD36拮抗组[(5.83±1.18)%与(7.63±1.64)%](均P0.05)。两组0 h时Mn-SOD活性无显著差异(P0.05);ox-LDL处理组24、48 h的Mn-SOD活性[分别为(17.17±2.90)%与(16.73±2.87)%]明显低于抗CD36拮抗组[(29.34±4.72)%与(22.83±3.72)%](均P0.05)。结论 CD36在ox-LDL所介导的脂质转运和代谢过程中具有重要作用。
[Abstract]:Objective to investigate the role of CD36 in reducing the activity of manganese (Mn) superoxide dismutase (SOD) in rats with oxidized low density lipoprotein (ox-LDL). Methods the rat RAW264.7 macrophages were divided into ox-LDL treatment group and anti CD36 antagonist group according to the treatment methods. The ox-LDL treatment group added 100 mg/L ox-LDL to the culture solution, and the anti CD36 antagonist group was first added in the culture solution. The apoptosis rate and Mn-SOD activity of two groups were compared after 2 mg/L anti CD36 m Ab pretreatment and then 100 mg/L ox-LDL. experiments. The apoptotic rate of 24,48 h in ox-LDL treatment group [10.8 + 2.91)% and (29.88 + 4.47)%] was significantly higher than that in the anti CD36 antagonist Group [(5.83 + 1.18)% and 7.63 + 1.64%]. There was no significant difference (P0.05); the Mn-SOD activity of 24,48 h in ox-LDL treatment group [17.17 + 2.90)% and (16.73 + 2.87)%] was significantly lower than that of the anti CD36 antagonist Group [(29.34 + 4.72)% and (22.83 + 3.72)%] (P0.05). Conclusion CD36 plays an important role in the lipid transport and metabolic pathway mediated by ox-LDL.

【作者单位】：邢台市人民医院普外科;
【分类号】：R54

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