高血压小鼠心肌肥厚过程中心肌组织Prx-2、8-OHdG蛋白表达变化及意义

发布时间：2018-05-14 05:26

本文选题：高血压 + 硫氧环蛋白过氧化物酶　；参考：《山东医药》2017年04期

【摘要】：目的探讨高血压小鼠心肌肥厚过程中心肌组织硫氧环蛋白过氧化物酶2(Prx-2)、8-羟基脱氧鸟嘌呤(8-OHd G)蛋白表达变化及其意义。方法将60只雄性C57BL/6N小鼠分为观察组40只和对照组20只,观察组采用两肾一夹(2K1C)法制备高血压心肌肥厚模型,对照组只分离左肾动脉,不放置肾动脉夹。两组分别于术前和术后2、4、8、12周测量尾动脉收缩压;术后2、4、8、12周分别随机选择10只观察组小鼠及5只对照组小鼠处死,计算全心质量指数和左心室质量指数,分别采用免疫印迹法、免疫组化法检测左心室心肌组织Prx-2、8-OHd G蛋白表达;分析观察组术后各时间点Prx-2、8-OHd G蛋白表达的关系。结果两组术前尾动脉收缩压比较无明显统计学差异(P0.05),观察组术后2、4、8、12周尾动脉收缩压、全心质量指数和左心室质量指数均呈升高趋势,且均高于对照组同时间点(P均0.05);证实建模成功。观察组术后2、4、8、12周左心室心肌组织Prx-2蛋白相对表达量及8-OHd G蛋白表达均呈升高趋势,且均高于对照组同时间点(P均0.05)。观察组术后2、4、8、12周左心室心肌组织8-OHd G和Prx-2蛋白表达均呈正相关(r分别为0.95、0.97、0.97和0.95,P均0.01)。结论高血压小鼠心肌肥厚过程中左心室心肌组织Prx-2、8-OHd G蛋白表达均升高,二者分别通过抗氧化和促氧化作用参与心肌肥厚的发生与发展。
[Abstract]:Objective to investigate the changes and significance of thioxocycline peroxidase (2Prx-2) -8-hydroxydeoxyguanine guanine 8-OHd (8-OHd) protein expression in myocardium of hypertensive mice during myocardial hypertrophy. Methods Sixty male C57BL/6N mice were divided into observation group (n = 40) and control group (n = 20). The hypertrophy model of hypertensive myocardium was established in the observation group with two kidneys and one clip (2K1C). The left renal artery was isolated in the control group, and the renal artery clamp was not placed in the control group. The systolic blood pressure of caudal artery was measured before operation and 12 weeks after operation, 10 mice in observation group and 5 mice in control group were randomly selected for 12 weeks after operation, and total heart mass index and left ventricular mass index were calculated by Western blotting. Immunohistochemical method was used to detect the expression of Prx-2OHd G protein in left ventricular myocardium, and the relationship between the expression of Prx-2OHd G protein at different time points after operation in the observation group was analyzed. Results there was no significant difference in the systolic blood pressure of caudal artery between the two groups before operation (P 0.05). In the observation group, the systolic blood pressure, total cardiac mass index (TMI) and left ventricular mass index (LVMI) increased significantly at 12 weeks after operation. All of them were higher than that of the control group at the same time point (P < 0.05), it was proved that the model was successful. The relative expression of Prx-2 protein and the expression of 8-OHd G protein in left ventricular myocardium in the observation group showed an increasing trend at 12 weeks after operation, and were higher than those in the control group at the same time point (P < 0.05). The expression of 8-OHd G and Prx-2 protein in left ventricular myocardium in the observation group was positively correlated with the expression of 8-OHd G and Prx-2 protein in left ventricular myocardium at 12 weeks after operation. The positive correlation was 0.95 卤0.970.97 and 0.95 卤0.95 respectively (P < 0.01). Conclusion the expression of Prx-2OHd G protein in left ventricular myocardium is increased during myocardial hypertrophy in hypertensive mice. Both of them participate in the occurrence and development of myocardial hypertrophy by anti-oxidation and pro-oxidation respectively.
【作者单位】：华北理工大学基础医学院;
【基金】：国家自然科学基金资助项目(81200188) 国家级大学生创新创业训练计划项目(201410081023)
【分类号】：R544.1

【相似文献】