舒洛地特对氧化型低密度脂蛋白诱导人脐静脉内皮细胞损伤的保护作用及其机制研究

发布时间：2018-05-14 15:08

本文选题：舒洛地特 + 氧化型低密度脂蛋白　；参考：《介入放射学杂志》2017年06期

【摘要】：目的研究舒洛地特(SDX)对氧化型低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞(HUVEC)损伤的保护作用及其机制。方法 CCK-8法确定ox-LDL干预HUVEC剂量及SDX药物浓度,活性氧(ROS)检测试剂盒验证SDX对HUVEC的保护作用。实时荧光定量-聚合酶链反应(RT-PCR)检测内皮型一氧化氮合酶(e NOS)、小凹蛋白(caveolin)-1 m RNA表达,免疫印迹试验检测e NOS、caveolin-1蛋白表达。Transwell试验检测HUVEC迁移能力,免疫印迹试验检测抗磷酸化e NOS(p-e NOS)蛋白表达。结果 100μg/ml ox-LDL刺激下,HUVEC细胞活力显著下降(P0.01),加入0.125 LRU/ml SDX后细胞活力明显改善(P0.01),ROS产生降低(P0.01)。SDX可下调ox-LDL损伤HUVEC后caveolin-1 m RNA和蛋白表达(P0.05),上调e NOS m RNA和蛋白、p-e NOS蛋白表达(P0.05),明显改善受损HUVEC迁移能力(P0.01)。结论 SDX通过调控caveolin-1/e NOS信号通路改善受损HUVEC细胞迁移能力,从而保护内皮细胞。
[Abstract]:Objective to study the protective effect of sulodide on human umbilical vein endothelial cells (HUVECs) induced by oxidized low density lipoprotein (ox-LDL) and its mechanism. Methods the dose of ox-LDL and the concentration of SDX were determined by CCK-8. The protective effect of SDX on HUVEC was verified by Ros assay kit. Real-time fluorescence quantification-polymerase chain reaction (RT-PCR) was used to detect the expression of endothelial nitric oxide synthase (ENOSN), caveolinone 1 m RNA, and the expression of e NOScaveolin-1 protein. Transwell assay was used to detect the migration ability of HUVEC. The expression of anti phosphorylated e NOS(p-e NOSs was detected by Western blotting assay. Results after the stimulation of 100 渭 g/ml ox-LDL, the viability of HUVEC cells decreased significantly (P 0.01). After adding 0.125 LRU/ml SDX, the cell viability was significantly improved. The decrease of Ros production was significantly decreased by P0.01G. SDX could down-regulate the expression of caveolin-1 m RNA and protein after ox-LDL damage HUVEC, and up-regulate the expression of e NOS m RNA and protein p-e NOS protein. P0.05 can obviously improve the migration ability of damaged HUVEC. Conclusion SDX can improve the migration ability of damaged HUVEC cells by regulating caveolin-1/e NOS signaling pathway and protect endothelial cells.
【作者单位】：上海交通大学医学院附属仁济医院血管外科;
【基金】：上海市科委国际科技合作基金(14430721400)
【分类号】：R543.5

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