Galectin-3对oxLDL诱导血管平滑肌细胞表型转化的调控

发布时间：2018-05-15 22:37

本文选题：oxLDL + Galectin-3　；参考：《上海交通大学》2015年硕士论文

【摘要】：背景:氧化低密度脂蛋白(ox LDL)与动脉粥样硬化病理过程中血管平滑肌细胞表型转化有关。在动脉粥样硬化病变过程中galectin-3也起着重要的作用。然而,对于galectin-3在氧化低密度脂蛋白诱导的血管平滑肌细胞表型转化中的作用目前知之甚少。方法:利用氧化低密度脂蛋白和galectin-3分别处理血管平滑肌细胞后观察细胞的表型变化。采用实时荧光定量PCR和Western blot的方法检测galectin-3,β-catenin,axin2,SMA,OPN和calponin等蛋白的表达。使用si RNA抑制galectin-3和β-catenin蛋白的表达。结果:氧化低密度脂蛋白可以促进galectin-3的表达及诱导血管平滑肌细胞向合成型转化。氧化低密度脂蛋白可以诱导OPN、SMA和calponin等蛋白的表达以及血管平滑肌细胞的迁移和增殖能力。利用si RNA沉默galectin-3的表达可以完全逆转氧化低密度脂蛋白诱导的血管平滑肌细胞的表型转化。Galectin-3的si RNA降低氧化低密度脂蛋白诱导的胞质和胞核中β-catenin蛋白的表达,但对于m RNA的表达没有影响。这些结果表明,氧化低密度脂蛋白诱导的血管平滑肌细胞表型转化和Wnt/β-catenin信号通路的激活依赖galectin-3。Galectin-3同样可以促进人脐动脉血管平滑肌细胞的表型转化和经典Wnt信号通路的激活。β-catenin的si RNA可以阻止galectin-3诱导的人脐动脉血管平滑肌细胞的表型转化。结论:氧化低密度脂蛋白通过galectin-3诱导了人脐动脉血管平滑肌细胞的表型转化,经典的Wnt信号通路在其中发挥重要作用。
[Abstract]:Background: oxidized low density lipoprotein (LDL) is associated with phenotypic transformation of vascular smooth muscle cells during atherosclerosis. Galectin-3 also plays an important role in atherosclerosis. However, little is known about the role of galectin-3 in phenotypic transformation of vascular smooth muscle cells induced by oxidized low density lipoprotein. Methods: the phenotypic changes of vascular smooth muscle cells were observed after treated with oxidized low density lipoprotein and galectin-3 respectively. The expression of galectin-3, 尾 -catenininaxin2 SMAOPN and calponin were detected by real-time fluorescence quantitative PCR and Western blot. Si RNA was used to inhibit the expression of galectin-3 and 尾-catenin. Results: oxidized low density lipoprotein could promote the expression of galectin-3 and induce the transformation of vascular smooth muscle cells to synthetic type. Oxidized low density lipoprotein can induce the expression of OPN SMA and calponin and the ability of migration and proliferation of vascular smooth muscle cells. Silencing the expression of galectin-3 by si RNA could completely reverse the phenotypic transformation of vascular smooth muscle cells induced by oxidized low density lipoprotein. The si RNA of Galectin-3 reduced the expression of 尾 -catenin protein in cytoplasm and nucleus induced by oxidized low density lipoprotein. But there was no effect on the expression of m RNA. These results show that Phenotypic transformation of vascular smooth muscle cells induced by oxidized low density lipoprotein and activation of Wnt/ 尾 -catenin signaling pathway dependent on galectin-3.Galectin-3 also promoted phenotypic transformation of human umbilical artery vascular smooth muscle cells and activation of classical Wnt signaling pathway. Si of 尾 -catenin RNA can prevent the phenotypic transformation of human umbilical artery smooth muscle cells induced by galectin-3. Conclusion: oxidized low density lipoprotein induces phenotypic transformation of human umbilical artery smooth muscle cells through galectin-3, in which classical Wnt signaling pathway plays an important role.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R543

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