心脏缝隙连接蛋白基因表达的节律性及其与生物钟系统的相关性研究

发布时间：2018-05-17 14:06

本文选题：缝隙连接蛋白基因 + 视上核　；参考：《宁波大学》2017年硕士论文

【摘要】：目的心律失常的发病存在显著的昼夜节律性,但其机制不明。缝隙连接蛋白对心脏电位传导具有重要作用,并是心电传导速度的重要决定因素。缝隙连接蛋白功能异常与心律失常的发生密切相关。本研究中,我们利用视上核损伤及自主神经组织大鼠模型,观察大鼠心脏内缝隙连接蛋白基因Cx40和Cx43的表达是否有节律性及其与生物钟系统的相关性。方法经过2周的单独饲养,分别制作视上核损伤及自主神经阻滞大鼠模型,每6小时采集各组动物的心房、心室组织,用荧光定量PCR法观察生物钟基因Bmal1和缝隙连接蛋白基因Cx40、Cx43的表达水平。结果本研究发现在野生型大鼠的心脏中,生物钟基因Bmal1及缝隙连接蛋白基因Cx40、Cx43的表达有显著的节律性;在视上核损伤大鼠心脏中,所有的生物钟基因和缝隙连接蛋白基因的表达节律均消失;在自主神经阻滞组,大鼠心脏内的生物钟基因表达节律仍存在,Cx40和Cx43表达节律性则消失。结论本研究显示缝隙连接蛋白基因Cx40和Cx43在心脏中呈节律性表达,这种节律性可能受到生物钟中枢的调节。
[Abstract]:Objective there is a significant circadian rhythm in the pathogenesis of arrhythmia, but its mechanism is unclear. Gap junction protein plays an important role in cardiac potential conduction and is an important determinant of ECG conduction velocity. Gap junction protein dysfunction is closely related to arrhythmia. In this study, we used the rat model of supraoptic nucleus injury and autonomic nerve tissue to observe whether the expression of gap junction protein gene Cx40 and Cx43 in rat heart was rhythmic and its correlation with the circadian clock system. Methods the rat models of supraoptic nucleus injury and autonomic nerve block were made by feeding alone for 2 weeks. The atrial and ventricular tissues of each group were collected every 6 hours. The expression of clock gene Bmal1 and gap junction protein gene Cx40Cx43 were detected by fluorescence quantitative PCR. Results it was found that the expression of clock gene Bmal1 and gap junction protein gene Cx40Cx43 was significantly rhythmic in the hearts of wild rats, and that in the hearts of rats with supraoptic nucleus damage, In the autonomic nerve block group, the circadian clock gene and gap junction protein gene expression rhythm still existed in the heart of the rats, but the rhythm of Cx40 and Cx43 expression disappeared in the autonomic nerve block group. Conclusion this study suggests that gap junction protein gene Cx40 and Cx43 express rhythmically in the heart, which may be regulated by the circadian center.
【学位授予单位】：宁波大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.7

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