新生小鼠心肌肌钙蛋白Ⅰ相互作用蛋白的鉴定与功能预测

发布时间：2018-05-25 06:05

本文选题：心肌肌钙蛋白Ⅰ + 蛋白质组学　；参考：《解放军医学杂志》2017年09期

【摘要】：目的采用高通量蛋白质组学筛选鉴定小鼠心肌原代细胞中可能与心肌肌钙蛋白I(c Tn I)存在相互作用的蛋白质并通过生物信息学软件预测其可能参与的生物过程及信号通路。方法取C57新生小鼠的心脏制备心肌原代细胞,提取细胞全蛋白行免疫共沉淀实验筛选出c Tn I的相互作用蛋白,通过质谱分析技术对其进行鉴定;将鉴定所得蛋白质信息输入生物信息学软件分析其理化性质,预测其可能参与的生物过程及信号通路。结果共鉴定出262种可能与c Tn I存在相互作用的蛋白质,预测其可能参与的生物进程14条,KEGG生物学通路33条(P0.05)。结论在小鼠心肌细胞中,有262种蛋白可能与c Tn I存在相互作用,其中部分蛋白可能参与心肌细胞钙离子内流及PI3K-Akt信号通路的调节。
[Abstract]:Objective to identify the proteins that may interact with cardiac troponin I (C Tn I) in murine cardiomyocytes by high throughput proteomics and to predict the possible biological processes and signaling pathways through bioinformatics software. Methods the cardiac primary cells were prepared from the heart of the newborn mice of C57, and the whole egg was extracted from the heart of the newborn mice of C57. The interaction protein of C Tn I was screened by the white line immunoprecipitation experiment, and was identified by mass spectrometry. The identification of protein information was introduced into bioinformatics software to analyze its physical and chemical properties, and to predict the possible biological processes and signal pathways involved in it. The results showed that 262 kinds of possible interactions with C Tn I were identified. Protein, prediction of its possible participation in biological processes 14, KEGG biological pathway 33 (P0.05). Conclusion in mouse cardiomyocytes, 262 proteins may interact with C Tn I, some of which may be involved in the regulation of calcium ion inflow and PI3K-Akt signaling pathway in cardiac myocytes.
【作者单位】：重庆医科大学附属儿童医院心脏内科儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地儿科学重庆市重点实验室;
【基金】：国家自然科学基金面上项目(81670212)~~
【分类号】：R542.2

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