艾塞那肽对高同型半胱氨酸血症大鼠主动脉组织Bcl-2与Bax表达影响的研究

发布时间：2018-05-25 18:43

本文选题：艾塞那肽 + 动脉粥样硬化　；参考：《广西医科大学》2016年硕士论文

【摘要】：目的：通过建立高同型半胱氨酸血症(Hyperhomocysteinemia, Hhcy)大鼠动物模型,用艾塞那肽进行干预,通过Western Blot方法观察艾塞那肽对高同型半胱氨酸血症大鼠主动脉组织B细胞淋巴瘤/白血病-2(B cell eukemia/lymphoma-2, Bel-2)和B细胞淋巴瘤/白血病-2相关蛋白X(Bcl-2-associated X protein Bax)表达的影响。方法：将36只SD大鼠随机分为3组：对照组、高同型半胱氨酸血症(Hhcy)组、艾塞那肽(EX)组,每组12只。利用蛋氨酸灌胃诱导的方法建立高同型半胱氨酸血症大鼠动物模型。对照组：大鼠标准饲料喂养,同等剂量饮用水灌胃,16周后处死；Hhcy组：大鼠标准饲料喂养,并将L-蛋氨酸溶于生理盐水,予以灌胃(1g/kg·d),16周后处死；艾塞那肽组：大鼠标准饲料喂养,并将L-蛋氨酸溶于生理盐水,予以灌胃(1g/kg·d),同时予艾塞那肽(1.0nmol/kg·d)腹膜下注射,16后周处死。静脉取血,并采集大鼠主动脉标本。应用广西医科大学全自动生化仪检测正常对照组、Hhcy组及艾塞那肽组大鼠的血浆同型半胱氨酸(Hcy)浓度、血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)和空腹血糖(FBG)；大鼠主动脉标本进行HE染色,观察各组大鼠主动脉组织的病理变化；应用Western Blot方法检测各组大鼠主动脉组织Bel-2、Bax的蛋白表达。结果：Hhcy组和艾塞那肽组大鼠血浆Hcy浓度明显高于对照组；艾塞那肽组大鼠血浆Hcy浓度明显低于Hhcy组,差异具有统计学意义(P0.05)；各组大鼠主动脉组织HE染色显示:Hhcy组和艾塞那肽组主动脉组织动脉粥样硬化程度均较对照组严重。艾塞那肽组大鼠主动脉组织动脉粥样硬化程度较Hhcy组减轻；Western Blot方法检测主动脉组织Bc 1-2和Bax的蛋白表达显示：Hhcy组、艾塞那肽组与对照组比较,Bcl-2的蛋白表达降低,Bax的蛋白表达增加；艾塞那肽组与Hhcy组比较,Bcl-2的蛋白表达明显升高,Bax的蛋白表达明显降低,差异具有统计学意义(P0.05)。结论：艾塞那肽有可能通过降低血浆中Hcy浓度,影响Bcl-2、Bax的蛋白表达,来抑制大鼠主动脉的内皮细胞调亡,改善大鼠主动脉粥样硬化的发生与发展。
[Abstract]:Objective: to establish a hyperhomocysteinemia model of rats with hyperhomocysteinemia (Hhcyia) and intervene with Isenapeptide. The effect of isenapeptide on the expression of B cell lymphoma / leukaemia B cell eukemia / lymphoma-2 (Bel-2) and B cell lymphoma / leukemia related protein (X(Bcl-2-associated X protein Bax) was observed by Western Blot in rats with hyperhomocysteinemia. Methods: Thirty-six SD rats were randomly divided into three groups: control group, hyperhomocysteinemia group and isenapeptide EXX group, with 12 rats in each group. The animal model of hyperhomocysteinemia was established by intragastric administration of methionine. Control group: rats were fed with standard diet, rats were killed by drinking water for 16 weeks, rats were fed with standard diet and L-methionine was dissolved in normal saline and killed after 16 weeks of administration of L-methionine. L-methionine was dissolved in normal saline and was given intragastric administration of 1 g / kg dU and intraperitoneal injection of isenapeptide 1.0 nmol / kg d) for 16 weeks. Blood samples were collected from vein and aorta of rats. The plasma homocysteine (Hcy) concentrations in the normal control group and the Isenapeptide group were detected by using the automatic biochemical instrument of Guangxi Medical University. Serum triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and fasting blood glucose (FBGG) were detected by HE staining. Western Blot method was used to detect the protein expression of Bel-2 and Bax in aorta of rats in each group. Results the plasma Hcy concentration of the rats in the two groups was significantly higher than that in the control group, and the concentration of plasma Hcy in the isenapeptide group was significantly lower than that in the Hhcy group. The difference was statistically significant (P 0.05), and HE staining showed that the degree of aortic atherosclerosis in the two groups was more serious than that in the control group. The degree of atherosclerosis in the aorta of the rats in the Isenapeptide group was less than that in the Hhcy group. The expression of Bcl-2 and Bax protein in aortic tissue was detected by Western Blot method. Compared with the control group, the expression of Bcl 2 protein in the Isenapeptide group was significantly lower than that in the control group, while the protein expression of Bcl 2 protein in the Isenapeptide group was significantly higher than that in the Hhcy group, and the difference was statistically significant (P 0.05). Conclusion: Isenapeptide may inhibit the apoptosis of rat aortic endothelial cells and improve the occurrence and development of aortic atherosclerosis by decreasing the concentration of Hcy in plasma and affecting the expression of Bcl-2P Bax protein.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R54

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