原发性高血压患者血清Chemerin水平及其与内皮功能的相关性

发布时间：2018-05-27 17:26

本文选题：原发性高血压 + 血管内皮功能　；参考：《河北医科大学》2016年硕士论文

【摘要】：目的:本课题通过检测原发性高血压(Essential hypertension,EH)患者血清Chemerin水平,阐明血清Chemerin水平与血管内皮功能的相关性。其中血管内皮功能用高敏C反应蛋白(High sensitivity C reactive protein,Hs CRP)、内皮素-1(Endothelin-1,ET-1)及一氧化氮(Nitricoxide,NO)来评估。方法:本课题实验组收集2015年1月至2015年12月就诊于河北医科大学第二医院门诊为原发性高血压的患者96例(男52例,女44例),高血压入选患者参照《2013年中国高血压防治指南》(修订版)的诊断标准分为三个亚组:a组(高血压1级,共33例,男18例,女15例),b组(高血压2级,共38例,男20例,女18例),c组(高血压3级,共25例,男14例,女11例)。同时选取对照组32例(男18例,女14例),对照组均选自河北医科大学第二医院的体检中心,体检血压正常,并记录入选者的病史和各项生化指标(包括Hs CRP)。采用酶联免疫吸附的方法(ELISA)测定所有入选者血清Chemerin、ET-1水平,再采用硝酸还原酶法测定受试者血清、NO的水平。实验数据采用SPSS19.0统计软件进行分析,计量资料采用均数±标准差()表示,两样本比较若符合正态性应用t检验,否则用Mann-Whitney U检验;多组间比较采用ANOVA法检验,若符合方差齐性,进一步两两比较采用LSD检验,方差不齐时组间比较采用Gamees-Howell法;Hs CRP、ET-1及NO与Chemerin之间的相关性采用pearson法进行相关分析;将Chemerin与具有相关意义的统计数据进行多元逐步直线回归分析。性别使用计数资料来表达,比较采用卡方检验。以P0.05为差异有统计学意义。结果:1与对照组相比,高血压组Chemerin水平显著高于对照组具有统计学差异(42.01±6.57 VS 117.93±4.89,P0.05);高血压亚组内各组Chemerin水平依次升高,具有统计学差异(128.10±20.30,180.63±18.44,239.59±31.86,P0.05)。2高血压组Hs CRP水平显著高于对照组具有统计学差异(2.92±0.74VS 4.29±0.97,P0.05);高血压亚组内Hs CRP水平无统计学差异(4.07±0.92,4.28±0.96,4.58±1.00,P0.05)。3高血压组ET-1水平显著高于对照组有统计学差异(41.12±11.24 VS90.56±20.34,P0.05);高血压各组内ET-1水平两两比较有统计学差异(77.32±15.68,87.89±13.07,112.13±17.60,P0.05)。4高血压组NO水平显著低于对照组有统计学差异(17.27±2.87 VS13.52±2.67,P0.05);高血压各组内NO水平比较c组低于b组及a组有统计学差异(10.00±1.71,14.38±1.53,15.19±1.67,P0.05),但b组与a组NO水平比较无统计学差异(P0.05)。5根据pearson相关分析表明:Chemerin水平与Hs CRP、ET-1水平呈正相关(相关系数r=0.225,r=0.561);Chemerin水平与NO水平呈负相关(相关系数r=-0.662);NO水平与ET-1水平呈负相关(r=-0.543)。结论:1与对照组相比,原发性高血压组血清Chemerin水平增多,并随血压升高而增多。2原发性高血压患者中血清Hs CRP水平及ET-1水平与Chemerin水平呈正相关;NO水平与Chemerin水平呈负相关。3血清Chemerin与血管内皮功能不全相关,并且血管内皮功能损伤程度越重,其血清水平越高,有可能成为反应内皮功能的新指标。
[Abstract]:Objective: to elucidate the correlation between serum Chemerin level and vascular endothelial function by detecting serum Chemerin levels in patients with Essential hypertension (EH), and the vascular endothelial function was characterized by high sensitivity C reactive protein (High sensitivity C reactive protein, Hs), endothelin and nitric oxide. Methods: tricoxide, NO). Methods: the experimental group collected 96 cases of primary hypertension (52 males and 44 females) from January 2015 to December 2015 at the second hospital of Hebei Medical University (52 men and 44 women). The diagnostic criteria for hypertension prevention and control in China were divided into three subgroups: a group (high blood pressure) Pressure 1, a total of 33 cases, 18 men, 15 women, B group (38 cases of hypertension, 38 cases, 20 men, 18 women), group C (hypertension 3, 25 cases, male 14, 11 cases). Meanwhile, the control group was selected from the Hebei Medical University medical center, the physical examination was normal, and records the history and the students of the candidates of the medical history and the students of the selected candidates and the students recorded the history and the students of selected candidates and the students of the selected students and the students recorded the history and the entry of selected students and the students of the selected history and the students of selected candidates of the history and the students of selected candidates and the students of the selected students and the students recorded the history and the entry of selected students and the students of selected students and the students of the history and the entries of selected candidates of the history and the students selected students The index (including Hs CRP). The serum Chemerin and ET-1 levels were measured by enzyme linked immunosorbent assay (ELISA). The serum and NO levels of the subjects were measured by the nitrate reductase method. The experimental data were analyzed with the SPSS19.0 statistical software, and the measured data were measured with mean standard deviation (). The two samples were compared with the normal state. T test was used sex, otherwise Mann-Whitney U test was used; ANOVA test was used in multiple groups. If it was consistent with the homogeneity of variance, further 22 was compared with LSD test and Gamees-Howell method was used when the variance was not homogeneous; the correlation analysis between Hs CRP, ET-1 and NO and Chemerin was related with Pearson method. The statistical data of the significance were analyzed by multiple stepwise linear regression. The sex use count data were used to express and compare with chi square test. The difference of P0.05 was statistically significant. Results: 1 compared with the control group, the level of Chemerin in the hypertension group was significantly higher than that of the control group (42.01 + 6.57 VS 117.93 + 4.89, P0.05); The level of Chemerin in each group increased in turn, with statistical difference (128.10 + 20.30180.63 + 18.44239.59 + 31.86, P0.05), Hs CRP in.2 hypertension group was significantly higher than that of the control group (2.92 + 0.74VS 4.29 + 0.97, P0.05), and there was no statistical difference between the Hs CRP level in the subgroup of hypertension (4.07 + 0.92,4.28 + 1, 1). The ET-1 level in the blood pressure group was significantly higher than that in the control group (41.12 + 11.24 VS90.56 + 20.34, P0.05), and the ET-1 level 22 in the hypertension group was significantly different (77.32 + 15.68,87.89 + 13.07112.13 + 17.60, P0.05) and the NO level in the.4 hypertension group was significantly lower than that of the control group (17.27 + 2.87 VS13.52 + 2.67, P0.05). Compared with group B and group A, there was a statistical difference between group C and group B (10 + 1.71,14.38 + 1.53,15.19 + 1.67, P0.05), but there was no statistical difference between B and a groups (P0.05). Negative correlation (correlation coefficient r=-0.662), NO level and ET-1 level were negatively correlated (r=-0.543). Conclusion: 1 compared with the control group, the serum Chemerin level in primary hypertension group increased and the serum Hs CRP level and ET-1 level were positively correlated with the level of Chemerin in the patients with.2 primary hypertension; NO level and Chemerin level were related. The negative correlation.3 serum Chemerin is associated with vascular endothelial dysfunction, and the more the vascular endothelial dysfunction is, the higher the level of the serum, the higher the serum level, which may be a new indicator of endothelial function.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R544.11

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