TRPV1在高同型半胱氨酸促内皮细胞凋亡中的作用

发布时间：2018-05-31 23:28

本文选题：TRPV1 + 同型半胱氨酸　；参考：《宁夏医科大学》2017年硕士论文

【摘要】：目的:研究TRPV1在高同型半胱氨酸促进内皮细胞凋亡中的作用,阐明辣椒素抗动脉粥样硬化的可能机制。方法:以人脐静脉内皮细胞为离体研究对象,不同浓度的同型半胱氨酸为干预措施(0mmol/L、0.05mmol/L、0.1mmol/L、0.2mmol/L、0.5mmol/L、1mmol/L、2mmol/L、4mmol/L)及不同时间(6h、12h、24h、36h、48h)干预后用CCK-8检测细胞增殖及活性情况,选择合适的同型半胱氨酸干预浓度及干预时间进一步分组并给予辣椒素干预,然后用细胞免疫荧光检测TRPV1蛋白是否在人血管内皮细胞上表达;Western blotting检测各组TRPV1蛋白的表达、TUNEL染色了解各组细胞凋亡情况。结果:(1)同型半胱氨酸对HUVECs的增殖能力有明显的抑制作用,吸光值从(1.03±0.12)渐下降至(0.42±0.05),且呈明显的剂量效应关系(P0.01);当干预浓度从0.5mmol/L开始、干预24小时后对人脐静脉内皮细胞增殖具有抑制作用(P0.05);(2)细胞免疫荧光检测在HUVECs上有TRPV1蛋白表达;(3)同型半胱氨酸干预后TPRV1在0.5mmol/L、1mmol/L、2mmol/L组表达较对照组下降(p0.05)、细胞凋亡较对照组增加(P0.05);(4)辣椒素干预后,与单纯同型半胱氨酸干预组比较,TRPV1蛋白表达升高(p0.05)、细胞凋亡率下降,且有统计学差异(P0.05)。结论:高同型半胱氨酸可通过下调TRPV1蛋白的表达,从而促进HUVECs凋亡;辣椒素可抑制高同型半胱氨酸诱导的内皮细胞凋亡。
[Abstract]:Aim: to investigate the role of TRPV1 in promoting apoptosis of endothelial cells induced by homocysteine, and to elucidate the possible mechanism of capsaicin against atherosclerosis. Methods: human umbilical vein endothelial cells (HUVECs) were treated with different concentrations of homocysteine at 0 mmol / L 0. 05 mmol / L 0. 1 mmol / L 0. 2 mmol / L ~ 0. 2 mmol / L ~ (2) mmol / L ~ 2 mmol / L ~ 4 mmol / L) and 6 h / 12 h / 24 h ~ 24 h / L ~ 36 h / L ~ 48 h). The appropriate concentration and duration of homocysteine intervention were further divided into groups and capsaicin was given. Then the expression of TRPV1 protein on human vascular endothelial cells was detected by immunofluorescence assay. The expression of TRPV1 protein in each group was detected by Tunel staining. Results (1) homocysteine had a significant inhibitory effect on the proliferation of HUVECs, and the absorptivity decreased from 1.03 卤0.12) to 0.42 卤0.05 渭 m, and showed a significant dose-effect relationship (P 0.01), and when the intervention concentration began with 0.5mmol/L, the concentration of homocysteine decreased from 1.03 卤0.12 to 0.42 卤0.05G, and the concentration of Hcy decreased from 1.03 卤0.12 to 0.42 卤0.05g. After 24 hours of intervention, the proliferation of human umbilical vein endothelial cells was inhibited by P0. 05 and P0. 05) the expression of TRPV1 protein was detected on HUVECs. The expression of TPRV1 was decreased in 0.5 mmol / L 1 mmol / L / L 2 mmol / L group compared with the control group (P 0. 05%), and the apoptosis of the cells was significantly lower than that of the control group (P 0. 05), and the expression of TPRV1 was significantly lower than that of the control group (P < 0. 05) after the intervention of homocysteine. In the exposure group, the concentration of capsaicin was increased by P0.05 (4) capsaicin. Compared with the control group, the expression of TRPV1 protein increased and the apoptosis rate decreased, and there was a significant difference in the expression of TRPV1 protein between the two groups. Conclusion: high homocysteine can promote the apoptosis of HUVECs by down-regulating the expression of TRPV1 protein, and capsaicin can inhibit the apoptosis of endothelial cells induced by high homocysteine.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R543.5

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