miR-221-3p在下肢缺血性疾病中的表达变化

发布时间：2018-06-02 01:23

本文选题：miR--p + 动脉硬化闭塞症　；参考：《中国动脉硬化杂志》2017年02期

【摘要】：目的探讨miR-221-3p在下肢缺血性疾病中的表达变化。方法临床试验:收集西南医科大学附属医院血管甲状腺外科2014年至2015年间55例动脉硬化闭塞症(ASO)患者和54例健康对照人群血浆,24例ASO截肢患者的正常血管及病变血管。选取3对年龄、性别相近的ASO患者和正常健康人血浆用Microarray筛选出ASO患者血浆中差异性表达在2倍以上的24种microRNA,其中miR-221-3p的表达量明显降低。用实时荧光定量PCR检测临床55例ASO患者和54例健康对照人群血浆及24例ASO截肢患者正常血管和病变血管中miR-221-3p的表达量。动物实验:选取SD大鼠,结扎组分离结扎大鼠股动脉制备下肢缺血模型,假手术组仅暴露分离股动脉不做结扎。分别于术前、术后即刻、术后7天、49天行激光多普勒扫描监测下肢皮肤血流变化。于术后7天、49天行激光多普勒扫描后处死各组大鼠,收集大鼠术侧腓肠肌及下腔静脉血,采用实时荧光定量PCR检测各组血浆和腓肠肌内miR-221-3p的表达量。结果临床试验显示:与健康对照组相比,ASO患者血浆中miR-22-3p的含量明显降低(1.078±0.119比0.617±0.121);与正常血管相比,病变血管中miR-221-3p的表达量也明显降低(1.017±0.113比0.625±0.136)。动物实验显示:与假手术组相比,结扎组7天时血浆和腓肠肌内miR-221-3p的含量明显降低(血浆1.04±0.07比0.31±0.07、腓肠肌1.01±0.07比0.64±0.09),49天时miR-221-3p的含量有所上升但仍低于对照组。结论 miR-221-3p在下肢缺血性疾病中明显降低,有望成为下肢缺血性疾病一个新的诊断方式及治疗靶点。
[Abstract]:Objective to investigate the expression of miR-221-3p in ischemic disease of lower extremity. Methods: a clinical trial was conducted in 55 patients with arteriosclerosis obliterans (ASO) and 54 healthy controls from 2014 to 2015 in Vascular thyroid surgery, affiliated Hospital of Southwest Medical University. The normal and diseased blood vessels were collected from 24 patients with ASO amputation. Three pairs of ASO patients of similar age and sex and normal controls were selected to select 24 microRNAs with different expression of ASO in plasma by Microarray, in which the expression of miR-221-3p was significantly decreased. The expression of miR-221-3p in normal and diseased vessels of 55 patients with ASO and 54 healthy controls and 24 patients with ASO amputation were detected by real-time fluorescence quantitative PCR. Animal experiment: SD rats were selected and the femoral artery was isolated and ligated in the ligation group to make the model of lower extremity ischemia. The sham operation group only exposed the isolated femoral artery and did not do the ligation. The changes of lower extremity skin blood flow were monitored by laser Doppler scanning at preoperative, postoperative and 7 ~ 49 days after operation. The rats were killed after laser Doppler scanning on the 7th day and 49th day after operation. The blood samples of gastrocnemius muscle and inferior vena cava were collected. The expression of miR-221-3p in plasma and gastrocnemius muscle was measured by real-time fluorescence quantitative PCR. Results compared with the healthy control group, the level of miR-22-3p in plasma was significantly decreased by 1.078 卤0.119 vs 0.617 卤0.121, and the expression of miR-221-3p in the diseased vessels was also significantly decreased by 1.017 卤0.113 to 0.625 卤0.136 compared with the normal blood vessels. Compared with the sham operation group, the content of miR-221-3p in plasma and gastrocnemius in ligation group was significantly lower than that in sham operation group (1.04 卤0.07 vs 0.31 卤0.07 in plasma and 1.01 卤0.07 vs 0.64 卤0.09 in gastrocnemius muscle at 49 days after ligation), but it was still lower than that in control group. Conclusion miR-221-3p may be a new diagnostic and therapeutic target for lower extremity ischemic diseases.
【作者单位】：西南医科大学附属医院血管甲状腺外科;西南医科大学附属医院中心实验室;成都市第五人民医院;
【基金】：四川省卫生计划生育委员会2014年科研基金项目(140035)
【分类号】：R543.5

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