原发性高血压患者血清CTRP5水平与血管内皮功能相关性研究

发布时间：2018-06-08 02:13

本文选题：原发性高血压 + 血管内皮功能　；参考：《河北医科大学》2017年硕士论文

【摘要】：目的:探究原发性高血压(EH)患者血清补体C1q肿瘤坏死因子相关蛋白5(CTRP5)水平变化,并分析其与血管内皮(VEC)功能的相关性。方法:本课题实验组收集2016年1月至2016年12月就诊于河北医科大学第二医院门诊的原发性高血压患者84例(男43例,女41例),入选患者参照《2013年中国高血压防治指南》(修订版)的诊断标准分为三个亚组:1组(高血压1级,共26例,男12例,女14例),2组(高血压2级,共28例,男16例,女12例),3组(高血压3级,共30例,男15例,女15例)。同时选取对照组44例(男20例,女24例),对照组均选自河北医科大学第二医院的体检中心,体检血压正常。记录入选者病史和各项生化指标(包括高敏C反应蛋白,HsCRP)。应用硝酸还原酶法测定血清一氧化氮(NO)的水平,应用酶联免疫吸附的方法(ELISA)测定血清CTRP5、内皮素(ET-1)水平。实验数据采用SPSS19.0统计软件进行分析,计量资料采用均数±标准差()表示,两样本比较若符合正态性应用t检验,否则应用Mann-Whitney U检验;多组间比较采用ANOVA法检验,若符合方差齐性,进一步两两比较采用LSD检验,方差不齐时组间比较采用Gamees-Howell法;CTRP5与HsCRP、ET-1及NO之间的相关性分析采用pearson法;性别使用计数资料来表达,比较采用卡方检验。以P0.05为差异有统计学意义。结果:1实验组CTRP5水平高于对照组,具有统计学差异(138.40±20.74ng/ml VS 106.37±9.53ng/ml,P0.05);实验组组内两两比较有统计学差异(124.08±16.96ng/ml,134.71±16.08ng/ml,154.26±16.88ng/ml,P0.05)。2实验组HsCRP水平显著高于对照组具有统计学差异(4.26±3.48mg/LVS 3.11±2.80mg/L,P0.05);实验组组内HsCRP水平3组高于1组及2组有统计学差异(3.19±2.80mg/L,3.77±2.72mg/L,5.65±4.21mg/L,P0.05),但1组与2组HsCRP水平无统计学差异(P=0.50)。3实验组ET-1水平显著高于对照组有统计学差异(75.56±20.33ng/LVS 26.87±3.98ng/L,P0.05);实验组组内两两比较有统计学差异(58.20±6.81ng/L,73.33±11.98ng/L,93.42±20.09ng/L,P0.05)。4实验组NO水平显著低于对照组有统计学差异(13.57±1.95umol/L VS31.83±6.69umol/L,P0.05);实验组组内NO水平两两比较有统计学差异(11.45±0.71umol/L,13.74±1.09umol/L,15.83±0.25umol/L,P0.05)。5根据pearson相关分析表明:CTRP5水平与HsCRP、ET-1水平呈正相关(相关系数r=0.273,P0.05;相关系数r=0.696,P0.05);CTRP5水平与NO水平呈负相关(相关系数r=-0.639,P0.05);NO水平与ET-1水平呈负相关(r=-0.796,P0.05)。结论:1血清CTRP5水平可以提示原发性高血压患者血管内皮功能的损伤程度,并有可能可以成为评价血管内皮功能的另一标志物。2血清CTRP5有可能成为心血管疾病的生物学标志物及治疗靶点。
[Abstract]:Aim: to investigate the changes of serum complement C1q tumor necrosis factor-associated protein 5 (CTRP5) in patients with essential hypertension (EH) and to analyze its correlation with vascular endothelial cell (VEC) function. Methods: from January 2016 to December 2016, 84 patients (43 males) with essential hypertension in the outpatient clinic of the second Hospital of Hebei Medical University were collected. According to the diagnostic criteria of the Chinese guidelines for the Prevention and treatment of Hypertension in 2013 (revised edition), 41 female patients were divided into three subgroups: group 1 (26 cases of grade 1 hypertension, 12 males and 14 females) (grade 2 hypertension, 28 cases, male 16 cases). There were 30 cases of hypertension, 15 cases of male and 15 cases of female. At the same time, 44 cases of control group (20 males and 24 females) were selected from the physical examination center of the second Hospital of Hebei Medical University. The blood pressure of the physical examination was normal. The history and biochemical parameters (including Gao Min C reactive protein) of the selected patients were recorded. Serum nitric oxide (no) was determined by nitrate reductase method and serum CTRP5 and endothelin ET-1 (et 1) by enzyme linked immunosorbent assay (Elisa). The experimental data were analyzed by SPSS 19.0 statistical software, and the measurement data were expressed by mean 卤standard deviation. If the two samples were in accordance with normal test, otherwise Mann-Whitney U test would be used, and the multigroup comparison would be tested by ANOVA method. If coincident with homogeneity of variance, further pairwise comparison was carried out by LSD test, and Gamees-Howell method was used to analyze the correlation between CTRP5 and HsCRPnET-1 and no, and the data of sex use counting was used to express it, and chi-square test was used to compare the data. P0.05 as the difference was statistically significant. Results the level of CTRP5 in the experimental group was higher than that in the control group. There was statistical difference between the experimental group (138.40 卤20.74ng/ml vs 106.37 卤9.53ng / ml P0.05) and the experimental group (124.08 卤16.96ng / ml vs 134.71 卤16.08ng / ml / ml = 154.26 卤16.88ng / ml / ml P 0.05.2 vs 4.26 卤3.48mg / L vs 3.11 卤2.80mg / L P0.05N respectively); the level of HsCRP in the experimental group was significantly higher than that in group 1 and group 2 (P < 0.05); the level of HsCRP in experimental group was significantly higher than that in group 1 and group 2 (P < 0.05). 3.19 卤2.80 mg / L = 3.77 卤2.72 mg / L = 5.65 卤4.21 mg / L P 0.05, but there was no significant difference in HsCRP level between group 1 and group 2. The level of ET-1 in experimental group was significantly higher than that in control group (75.56 卤20.33ngLVS 26.87 卤3.98ngL / L P 0.05N), and the level of no in experimental group was significantly lower than that in control group (58.20 卤6.81ngLP / P 73.33 卤11.98ngL / L 93.42 卤20.09ngL / L = 0.05.4). The statistical difference was 13.57 卤1.95umoll / L VS31.83 卤6.69umoll / L P 0.05g / L and 11.45 卤0.71umoll / L / L = 13.74 卤1.09umolol / L = 15.83 卤0.25umolr / L P 0.05.5 according to pearson correlation analysis, there was a positive correlation between the level of CTRP5 and the level of HsCRPnET-1 (r = 0.273g / L, P 0.05; r = 0.696P 0.05P = 0.05; r = 0.696P 0.05g / L = 13.74 卤1.09umolol / L = 15.83 卤0.25umolr / L P 0.05.5). According to pearson correlation analysis, the correlation between the level of CTRP5 and the level of HsCRPt-1 was positively correlated. There was a negative correlation between the level of no and the level of ET-1. Conclusion the serum CTRP5 level in the patients with essential hypertension may indicate the degree of injury of vascular endothelial function in patients with essential hypertension. Serum CTRP5 may be a biomarker and therapeutic target for cardiovascular diseases.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R544.11

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